ID W5Q0S1_SHEEP Unreviewed; 582 AA.
AC W5Q0S1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=2-hydroxyacyl-CoA lyase 1 {ECO:0008006|Google:ProtNLM};
GN Name=HACL1 {ECO:0000313|Ensembl:ENSOARP00000016307.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000016307.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000016307.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000016307.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000016307.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-hydroxyoctadecanoyl-CoA = formyl-CoA + heptadecanal;
CC Xref=Rhea:RHEA:55196, ChEBI:CHEBI:57376, ChEBI:CHEBI:74116,
CC ChEBI:CHEBI:138631; Evidence={ECO:0000256|ARBA:ARBA00000194};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55197;
CC Evidence={ECO:0000256|ARBA:ARBA00000194};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- SIMILARITY: Belongs to the TPP enzyme family.
CC {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
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DR EMBL; AMGL01012557; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012558; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012559; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5Q0S1; -.
DR SMR; W5Q0S1; -.
DR STRING; 9940.ENSOARP00000016307; -.
DR PaxDb; 9940-ENSOARP00000016307; -.
DR Ensembl; ENSOART00000016540.1; ENSOARP00000016307.1; ENSOARG00000015192.1.
DR eggNOG; KOG1185; Eukaryota.
DR Proteomes; UP000002356; Chromosome 1.
DR Bgee; ENSOARG00000015192; Expressed in duodenum and 54 other cell types or tissues.
DR ExpressionAtlas; W5Q0S1; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR CDD; cd02004; TPP_BZL_OCoD_HPCL; 1.
DR CDD; cd07035; TPP_PYR_POX_like; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR045025; HACL1-like.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR PANTHER; PTHR43710; 2-HYDROXYACYL-COA LYASE; 1.
DR PANTHER; PTHR43710:SF2; 2-HYDROXYACYL-COA LYASE 1; 1.
DR Pfam; PF02775; TPP_enzyme_C; 1.
DR Pfam; PF00205; TPP_enzyme_M; 1.
DR Pfam; PF02776; TPP_enzyme_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 18..131
FT /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF02776"
FT DOMAIN 207..336
FT /note="Thiamine pyrophosphate enzyme central"
FT /evidence="ECO:0000259|Pfam:PF00205"
FT DOMAIN 405..561
FT /note="Thiamine pyrophosphate enzyme TPP-binding"
FT /evidence="ECO:0000259|Pfam:PF02775"
SQ SEQUENCE 582 AA; 63665 MW; 44DB674FA71E42E0 CRC64;
MAGGDRAGRS GGREGQVSGA RVIAQALKTQ DVNYMFGIVG IPVTEIALAA QEVGIRYIGM
RNEQAACYAA SAVGYLTGRP GVCLVVSGPG LIHGLGGMAN ANMNCWPLIV IGGSSERSQE
TMGAFQEFPQ VEACRLYSKF SARPSSIEAI PSIIEKAVRS SIYGRPGACY VDIPADFVNL
QVNVNSIKYV ECCMPPPVSM AETSAVRMAA SVIRNAKQPL VIIGKGAAYA HAEESIRKLV
EQCKLPFLPT PMGKGVIRDN HPNCVAAARS SRALQFADVI VLLGARLNWI LHFGLPPRYQ
PDVKFIQVDI CAEELGNNVR PAVTLLGDIN AVTKQLLEQF DKTPWQYPPE SEWWKVLREK
MKSNEALSKE LASKKSLPMN YYTVFYHVQE QLPRDCFVVS EGANTMDIGR TVLQNYLPRH
RLDAGTFGTM GVGLGFAIAA AIVAKDRNPG KRVICVEGDS AFGFSGMEVE TICRYNLPIV
LLVVNNNGIY QGFDTDSWKE MLKFGDATTV APPMSLLPHS HYEHVMTAFG GKGYFVQTPE
ELQKSLRQSL ADTTNPSLIN IMIEPQATRK AQVRHLSTRM YH
//
