ID W5Q1Q6_SHEEP Unreviewed; 1005 AA.
AC W5Q1Q6;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Phosphoinositide phospholipase C {ECO:0000256|RuleBase:RU361133};
DE EC=3.1.4.11 {ECO:0000256|RuleBase:RU361133};
GN Name=PLCL2 {ECO:0000313|Ensembl:ENSOARP00000016644.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000016644.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000016644.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000016644.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000016644.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-
CC diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:58456,
CC ChEBI:CHEBI:203600; EC=3.1.4.11;
CC Evidence={ECO:0000256|ARBA:ARBA00023674};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33180;
CC Evidence={ECO:0000256|ARBA:ARBA00023674};
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DR EMBL; AMGL01012655; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012656; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012657; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012658; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012659; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012660; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012661; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01012664; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5Q1Q6; -.
DR SMR; W5Q1Q6; -.
DR STRING; 9940.ENSOARP00000016644; -.
DR PaxDb; 9940-ENSOARP00000016644; -.
DR Ensembl; ENSOART00000016881.1; ENSOARP00000016644.1; ENSOARG00000015517.1.
DR eggNOG; KOG0169; Eukaryota.
DR HOGENOM; CLU_002738_0_1_1; -.
DR OMA; MRTSWIS; -.
DR Proteomes; UP000002356; Chromosome 1.
DR Bgee; ENSOARG00000015517; Expressed in longissimus thoracis muscle and 55 other cell types or tissues.
DR GO; GO:0050811; F:GABA receptor binding; IEA:InterPro.
DR GO; GO:0070679; F:inositol 1,4,5 trisphosphate binding; IEA:InterPro.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:UniProtKB-EC.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0032228; P:regulation of synaptic transmission, GABAergic; IEA:InterPro.
DR CDD; cd00275; C2_PLC_like; 1.
DR CDD; cd16223; EFh_PRIP2; 1.
DR CDD; cd13364; PH_PLC_eta; 1.
DR CDD; cd08597; PI-PLCc_PRIP_metazoa; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR001192; PI-PLC_fam.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR015359; PLC_EF-hand-like.
DR InterPro; IPR028382; PLCL2_EFh.
DR InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR PANTHER; PTHR10336:SF84; INACTIVE PHOSPHOLIPASE C-LIKE PROTEIN 2; 1.
DR PANTHER; PTHR10336; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF09279; EF-hand_like; 1.
DR Pfam; PF16457; PH_12; 1.
DR Pfam; PF00388; PI-PLC-X; 1.
DR Pfam; PF00387; PI-PLC-Y; 1.
DR PRINTS; PR00390; PHPHLIPASEC.
DR SMART; SM00239; C2; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00148; PLCXc; 1.
DR SMART; SM00149; PLCYc; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
DR PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|RuleBase:RU361133};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963,
KW ECO:0000256|RuleBase:RU361133};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361133};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Transducer {ECO:0000256|ARBA:ARBA00023224}.
FT DOMAIN 32..142
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 509..625
FT /note="PI-PLC Y-box"
FT /evidence="ECO:0000259|PROSITE:PS50008"
FT DOMAIN 625..754
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 977..1005
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 983..1005
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1005 AA; 113729 MW; DD87AC8F2E6F1FAC CRC64;
DGSKQKRERK KTVSFSSMPT EKKISSASDC ISAMVEGSEL KKVRSNSRIY HRYFLLDADM
QSLRWEPSKK DSEKAKIDIK SIKEVRTGKN TDIFRSNGVS DQISEDCAFS VIYGENYESL
DLVANSADVA NIWVTGLRYL ISYGKHTLDM LESSQDNMRT SWVSQMFSEI DVDNLGHITL
CSAVQCIRNL NPGLKTSKIE LKFKELHKSK DKTGTDVTKE EFVEVFHELC TRPEIYFLLV
QFSSNKEFLD TKDLMMFLEA EQGVAHINEE ISLEIIHKYE PSKEGQEKGW LSIDGFTNYL
MSPDCYIFDP EHKKVCQDMK QPLSHYFINS SHNTYLIEDQ FRGPSDITGY IRALKMGCRS
VELDVWDGPD NEPVIYTGHT MTSQIVFRSV IDIINKFAFF ASEYPLILCL ENHCSIKQQK
VMVRHMKKIL GDKLYTASPN VEESYLPSPD VLKGKILVKA KKLSSSCSGV EGDVTDEDEG
AEMSQRMGKE STELPNHLPA KRFQLCKELS ELVSICKSVQ FKEFQVSFQA QKYWEVCSFN
EVLASKYANE NPGDFVNYNK RFLARVFPSP MRIDSSNMNP QDFWKCGCQI VAMNFQTPGL
MMDLNVGWFR QNGNCGYVLR PAIMREEVSF FSANTKDSVP GVSPQLLHIK VISGQNFPKP
KGSGAKGDVV DPYVYVEIHG IPADCAEQRT RTVHQNGDAP IFDESFEFQV NLPELAMVRF
VVLDDDYIGD EFIGQYTIPF ECLQTGLAHA SLFVHVAITN RRGGGKPHKR GLSVRKGKKS
REYASLRTLW IKTVDEVFKN AQPPIRDATD LRENMQNAVV SFKELCGLSS VANLMQCMLA
VSPRFLGPDN TPLVVLNLSE QYPTMELQGI VPEVLKKIVT TYDMMIQSLK ALIENADAVY
EKIVHCQKAA MEFHEHLQSI GTREGLKERK LQKAVESFTW NITILKGQAD LLKYAKNETV
ENLKQIHYAA VSCGLNKPGT ENADAPKPRR SLEVIPEKAN DETGA
//
