ID W5Q9C5_SHEEP Unreviewed; 1026 AA.
AC W5Q9C5;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=Leucyl-cystinyl aminopeptidase {ECO:0008006|Google:ProtNLM};
GN Name=LNPEP {ECO:0000313|Ensembl:ENSOARP00000019317.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000019317.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000019317.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000019317.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000019317.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}.
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR EMBL; AMGL01094771; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01094772; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01094773; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01094774; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01094775; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_012034381.1; XM_012178991.2.
DR AlphaFoldDB; W5Q9C5; -.
DR STRING; 9940.ENSOARP00000019317; -.
DR PaxDb; 9940-ENSOARP00000019317; -.
DR Ensembl; ENSOART00000019587.1; ENSOARP00000019317.1; ENSOARG00000017994.1.
DR GeneID; 101102729; -.
DR KEGG; oas:101102729; -.
DR CTD; 4012; -.
DR eggNOG; KOG1046; Eukaryota.
DR HOGENOM; CLU_003705_2_2_1; -.
DR OMA; ERFFLSM; -.
DR OrthoDB; 3085317at2759; -.
DR Proteomes; UP000002356; Chromosome 5.
DR Bgee; ENSOARG00000017994; Expressed in thymus and 55 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533:SF42; LEUCYL-CYSTINYL AMINOPEPTIDASE; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 174..358
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 393..611
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 690..1009
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 465
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 464
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 468
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 487
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 550
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1026 AA; 116996 MW; 3181EC4EED515227 CRC64;
MEPFTNDRLQ LPRNMIENSM FEEEPDVVDL AKEPCLHPLE PDEVEYEPRG SRLLVRGLGE
HELDEDEEDY ESSAKLLGMS FMNRSSGLRN SAAGYRPSSD GTCSAHSART VGVCIFIIIV
AVAIIMMIYL LPRCIFTKEG CHKKNQPMEP IQPIATNGKL FPWAQIRLPT AVAPVRYDLT
LHPNLTSMTF RGSVTISLQA LQATWNIVLH STGHNISRVT FMTAVSSQEK EVDVLEYPLH
EQIAIVAPET LLEGHNYTLK IEYSANISSS SYGFYGISYR DESNEKKYFA ATQFEPLAAR
SAFPCFDEPA FKATFVIRIK RDEQYTALSN MPKKSSVVLE DGLVQDEFFE SVKMSTYLVA
FIVGEMKNLS QDVNGTLVSI YAVPEKIGQV HHALETTVKL LDFYQNYFEI QYPLKKLDLV
AIPDFEAGAM ENWGLLTFRE ETLLYDGNTS SVADRKLVTK IIAHELAHQW FGNLVTMQWW
NDLWLNEGFA TFMEYFSLEK IFGELSSYED FLDARFKTMR KDALNSSHPI SSSSVQSSEQ
IEEMFDSLSY FKGASLLLML KTYLSEDIFQ HAIILYLHNH SYTSTHSDDL WDSFNESTNK
TLDVKKMMRT WTLQKGFPLV TVQRKGKQIL VQQERFSLNV KPEIQPSDAS SLWHIPLSYV
TDGKNYSKHR SVSLLDKKSG VINLTEEVQW IKVNTNMTGY YIVHYADDNW EALIKQLKTN
PYVLSDKDRA NLINNIFELA GLGKVSLQRA FDLIDYLRNE TYTAPISEAL FQTELIYNLL
DKLGHMDLAS RLVNKVFKLL QSQIQQQTWT DEGTPSTREL RTVLLDFACT HSLENCSAAA
LKLFNDWMAS NGTQSLPTDV MATVFKAGAK TEGGWSFLLS KYVSLGSEAE KNKILKALAS
SEDVRKLYWL MKKSLDGDLI RTQKLSFIIR TVARHFPGHL LAWDFVKENW SKLVQKFHLG
SYTIQSIVAG STHLFSTKAH LSEVQAFFEN QSEATFQLRC VQEALEVIQL NMQWVEKNLK
TLTYWL
//
