ID W5UDI8_ICTPU Unreviewed; 659 AA.
AC W5UDI8;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Galactocerebrosidase {ECO:0000256|ARBA:ARBA00019657};
DE EC=3.2.1.46 {ECO:0000256|ARBA:ARBA00012657};
DE AltName: Full=Galactosylceramidase {ECO:0000256|ARBA:ARBA00033098};
GN Name=galc {ECO:0000313|EMBL:AHH39940.1};
GN Synonyms=galca {ECO:0000313|RefSeq:XP_017312088.1};
OS Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Ictaluridae; Ictalurus.
OX NCBI_TaxID=7998 {ECO:0000313|EMBL:AHH39940.1};
RN [1] {ECO:0000313|EMBL:AHH39940.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Mixed {ECO:0000313|EMBL:AHH39940.1};
RX PubMed=23127152; DOI=10.1186/1471-2164-13-595;
RA Liu S., Zhang Y., Zhou Z., Waldbieser G., Sun F., Lu J., Zhang J.,
RA Jiang Y., Zhang H., Wang X., Rajendran K.V., Khoo L., Kucuktas H.,
RA Peatman E., Liu Z.;
RT "Efficient assembly and annotation of the transcriptome of catfish by RNA-
RT Seq analysis of a doubled haploid homozygote.";
RL BMC Genomics 13:595-595(2012).
RN [2] {ECO:0000313|RefSeq:XP_017312088.1}
RP IDENTIFICATION.
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_017312088.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a D-galactosylceramide + H2O = an N-acyl-sphingoid base + D-
CC galactose; Xref=Rhea:RHEA:43412, ChEBI:CHEBI:4139, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:36498, ChEBI:CHEBI:83273;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43413;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-galactosyl-(1<->1)-sphing-4-enine + H2O = D-galactose +
CC sphing-4-enine; Xref=Rhea:RHEA:43908, ChEBI:CHEBI:4139,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:57756, ChEBI:CHEBI:57934;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43909;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 59 family.
CC {ECO:0000256|ARBA:ARBA00005637}.
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DR EMBL; JT412651; AHH39940.1; -; mRNA.
DR RefSeq; XP_017312088.1; XM_017456599.3.
DR STRING; 7998.ENSIPUP00000008923; -.
DR Ensembl; ENSIPUT00000009312; ENSIPUP00000008923; ENSIPUG00000006035.
DR GeneID; 108258153; -.
DR KEGG; ipu:108258153; -.
DR CTD; 449649; -.
DR OMA; HEAWDEN; -.
DR OrthoDB; 5487508at2759; -.
DR Proteomes; UP000221080; Chromosome 25.
DR GO; GO:0005764; C:lysosome; IEA:Ensembl.
DR GO; GO:0004336; F:galactosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006683; P:galactosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR049162; GH59_C.
DR InterPro; IPR049161; GH59_cat.
DR InterPro; IPR001286; Glyco_hydro_59.
DR InterPro; IPR035394; Glyco_hydro_59_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR15172; GALACTOCEREBROSIDASE; 1.
DR PANTHER; PTHR15172:SF1; GALACTOCEREBROSIDASE; 1.
DR Pfam; PF02057; Glyco_hydro_59; 1.
DR Pfam; PF21708; Glyco_hydro_59_C; 1.
DR Pfam; PF17387; Glyco_hydro_59M; 1.
DR PRINTS; PR00850; GLHYDRLASE59.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Sphingolipid metabolism {ECO:0000256|ARBA:ARBA00022919}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..659
FT /note="Galactocerebrosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013540188"
FT DOMAIN 33..326
FT /note="Glycosyl hydrolase family 59 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02057"
FT DOMAIN 334..449
FT /note="Glycosyl hydrolase family 59 central"
FT /evidence="ECO:0000259|Pfam:PF17387"
FT DOMAIN 485..658
FT /note="Glycosyl hydrolase family 59 C-terminal lectin"
FT /evidence="ECO:0000259|Pfam:PF21708"
FT ACT_SITE 176
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
FT ACT_SITE 251
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
SQ SEQUENCE 659 AA; 73769 MW; 57001183FAAEB49A CRC64;
MNSYVVLFLF SFIRLCVTEK YVLDDRLGLG RQFDGIGGLS GGGATSRLLI NYKEPYRSQI
LDYLFKPNFG ASLHILKVEI GGDSQTTDGT EPSHMHNETD ENYFRGYEWW LMKEAKQRNP
NITLIGLPWA FPGWVGNGSK WPYNFPDITA NYVVSWVIGA KQYHGLDIDY IGVWNERTFD
SKYIQVLRNK LDQADLPNIG IIAADGNWDI SNAMLVDPYL NDAVEIIGVH YPGTMTVKEA
LLTDKKLWSS EDYSTFNNDI GAGCWARILN QNYVNGRMTS TISWNLIASY YEDLSFGRDG
LMTAEEPWSG NYVAETPIWI TAHTTQFAQP GWTYLQTIGN LTRGGSYVAL TDGQGNLTII
IETMTHNHSQ CIRPPLPPFS VSPQEATFQL KGSFAAVNGL QLWYSKLDFN TKKDVLFKKA
DPIKVSNGCF TLKLDVDEIY TITTVTTGQK GLYPNPPDSA SFPKKYFDDF NVQNPPFSEA
PYFADQTGVF EYFTNLMDPG PHNLTLRQVV NQQPVSWGYD ADQTISIIGD HGWQDVSVSC
DIYLETEDGG VFVAARVDQS GESVRRAKGV FYWVFANGTY KVTNDIGGEK VLAEGLSGTR
PGIWHTLTLI VKGNYAFGML NGFPLWKNAV VLQPKNGWAA IGTSSFEFAQ FDNFLVDAD
//
