ID W5XXT1_9CORY Unreviewed; 613 AA.
AC W5XXT1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=B843_02245 {ECO:0000313|EMBL:AHI21841.1};
OS Corynebacterium vitaeruminis DSM 20294.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=1224164 {ECO:0000313|EMBL:AHI21841.1, ECO:0000313|Proteomes:UP000019222};
RN [1] {ECO:0000313|EMBL:AHI21841.1, ECO:0000313|Proteomes:UP000019222}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10234 {ECO:0000313|Proteomes:UP000019222};
RA Ruckert C., Albersmeier A., Kalinowski J.;
RT "The complete genome sequence of Corynebacterium vitaeruminis DSM 20294.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; CP004353; AHI21841.1; -; Genomic_DNA.
DR AlphaFoldDB; W5XXT1; -.
DR STRING; 1224164.B843_02245; -.
DR KEGG; cvt:B843_02245; -.
DR PATRIC; fig|1224164.3.peg.442; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_004744_4_1_11; -.
DR Proteomes; UP000019222; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000019222}.
FT DOMAIN 137..517
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 613 AA; 68102 MW; 0DD2CA8781E0E886 CRC64;
MVADEVTRFH VWAPNAESVQ LLVFNGEGGT QEALGMTRGD RGEWSVELAG NHEGLEYLYR
VDGREAVDPY ARAVTANGLR SVAIDVDKLL GTATRLDSVG DPGNAIIYEA HVRDLTIAPG
NGIAHKGKFL GLAEEGRRTA RGNLSGLDYI ASLGITHLQL LPIFDFGSVD ELGDLAFDAQ
YNWGYDPNNY NVPEGSYSTD PRDPYARLRE LRQLVDACHA RGLRVIMDVV YNHVYDARTN
PFELIEPGYY FRKDAWGDFY DATVCGNETA SERPMMRRFI VDSVRYWART FGLDGFRFDL
MGIHDVETMN AVRAALDEID PGIILLGEGW AMGNHAPGVV PADQRAGALM PRIAMFNDTF
RDVVKGSNFH IEGAGFVSGN PSYLPDDADP WTASPAAEEL FDAMRGSLTQ RDYLTAAQSV
VYNESHDNYT MFDKLRGTAG LEHASLAEIA RRHTLATTIQ LLSRGIAFVH AGQELLRTKQ
GEENSYRSPD HVNAFDYDRA EQFAPQVAFF RSLVAFRRQW SWVTETDYEA IDVTTVPVEA
SGLHLSYRVK RAFDGNDAWV LINADSGLWQ APIAAGEYRV HICDEHVVED PAKVRFGEEF
PVAALSVVVL EKL
//
