ID W6KF19_9TRYP Unreviewed; 503 AA.
AC W6KF19;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012386};
DE EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
GN ORFNames=GSEM1_T00000241001 {ECO:0000313|EMBL:CCW59713.1};
OS Phytomonas sp. EM1.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Phytomonas.
OX NCBI_TaxID=479712 {ECO:0000313|EMBL:CCW59713.1, ECO:0000313|Proteomes:UP000027080};
RN [1] {ECO:0000313|EMBL:CCW59713.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=EM1 {ECO:0000313|EMBL:CCW59713.1};
RA Genoscope - CEA;
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CCW59713.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EM1 {ECO:0000313|EMBL:CCW59713.1};
RX PubMed=24516393; DOI=10.1371/journal.pgen.1004007;
RA Porcel B.M., Denoeud F., Opperdoes F., Noel B., Madoui M.A.,
RA Hammarton T.C., Field M.C., Da Silva C., Couloux A., Poulain J.,
RA Katinka M., Jabbari K., Aury J.M., Campbell D.A., Cintron R., Dickens N.J.,
RA Docampo R., Sturm N.R., Koumandou V.L., Fabre S., Flegontov P., Lukes J.,
RA Michaeli S., Mottram J.C., Szoor B., Zilberstein D., Bringaud F.,
RA Wincker P., Dollet M.;
RT "The Streamlined Genome of Phytomonas spp. Relative to Human Pathogenic
RT Kinetoplastids Reveals a Parasite Tailored for Plants.";
RL PLoS Genet. 10:e1004007-e1004007(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00024168};
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD2 family.
CC {ECO:0000256|ARBA:ARBA00034489}.
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DR EMBL; HF955061; CCW59713.1; -; Genomic_DNA.
DR AlphaFoldDB; W6KF19; -.
DR EnsemblProtists; CCW59713; CCW59713; GSEM1_T00000241001.
DR OrthoDB; 169000at2759; -.
DR Proteomes; UP000027080; Unassembled WGS sequence.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR InterPro; IPR005636; DTW.
DR InterPro; IPR039262; DTWD2/TAPT.
DR PANTHER; PTHR21392:SF0; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2; 1.
DR PANTHER; PTHR21392; UNCHARACTERIZED; 1.
DR Pfam; PF03942; DTW; 1.
DR SMART; SM01144; DTW; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000027080};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 129..371
FT /note="DTW"
FT /evidence="ECO:0000259|SMART:SM01144"
SQ SEQUENCE 503 AA; 56510 MW; CE34547291A293BC CRC64;
MHLTDGEVES NPYKQSMLRL LDYIGLRGYG TSEATMPERS AAEQERIAQH LLRVTQEAQG
KSTHTGTLGF ALRYGSITTP DAAAQPTIYL SAEETLEWKA FLAGTPVKER HMVSVRRRLS
LNLWRAHIRG LCLWCWFPMG VCMCAKLREY RAALPAEALS GAFQLTILLH AEEVFRSTNT
GHLAAFLLGA PLRVWGVPED DTYIAQLPAT SEYATIASVT VEGGQHRSCD APYRVHHLLL
YPERGAPELD GFIHDRLHTE PVGAPRTSAS GVSSAPPSAD KFHLILLDST WGQALSLNRH
ISRCVPRVSL TISKEYEALF SALRKRTRES GVSTFEAISM AIEQHLHALG MHDASAFVFH
TLIGAMKEFV DMRCLLKFTE PRFEQEGDKL KEIVQQRDLA RREEAQHRQE LLASRMQTDA
ATRRLLLPPV LNYCYCCDRV IGWHRVPEHV LGRRHHSALS LNPTCEPSKM ARSCFIADFS
RLPRSQYAEG HHGEKEEGEV KRG
//
