ID W6KIA5_9TRYP Unreviewed; 454 AA.
AC W6KIA5;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Plastid-encoded RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00031776};
GN ORFNames=GSEM1_T00004262001 {ECO:0000313|EMBL:CCW60788.1};
OS Phytomonas sp. EM1.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Phytomonas.
OX NCBI_TaxID=479712 {ECO:0000313|EMBL:CCW60788.1, ECO:0000313|Proteomes:UP000027080};
RN [1] {ECO:0000313|EMBL:CCW60788.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=EM1 {ECO:0000313|EMBL:CCW60788.1};
RA Genoscope - CEA;
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CCW60788.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EM1 {ECO:0000313|EMBL:CCW60788.1};
RX PubMed=24516393; DOI=10.1371/journal.pgen.1004007;
RA Porcel B.M., Denoeud F., Opperdoes F., Noel B., Madoui M.A.,
RA Hammarton T.C., Field M.C., Da Silva C., Couloux A., Poulain J.,
RA Katinka M., Jabbari K., Aury J.M., Campbell D.A., Cintron R., Dickens N.J.,
RA Docampo R., Sturm N.R., Koumandou V.L., Fabre S., Flegontov P., Lukes J.,
RA Michaeli S., Mottram J.C., Szoor B., Zilberstein D., Bringaud F.,
RA Wincker P., Dollet M.;
RT "The Streamlined Genome of Phytomonas spp. Relative to Human Pathogenic
RT Kinetoplastids Reveals a Parasite Tailored for Plants.";
RL PLoS Genet. 10:e1004007-e1004007(2014).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|ARBA:ARBA00004026}.
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000256|ARBA:ARBA00026088}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase
CC subunit family. {ECO:0000256|ARBA:ARBA00025804}.
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DR EMBL; HF955063; CCW60788.1; -; Genomic_DNA.
DR AlphaFoldDB; W6KIA5; -.
DR EnsemblProtists; CCW60788; CCW60788; GSEM1_T00004262001.
DR OrthoDB; 5472982at2759; -.
DR Proteomes; UP000027080; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0001054; F:RNA polymerase I activity; IEA:InterPro.
DR GO; GO:0001056; F:RNA polymerase III activity; IEA:InterPro.
DR CDD; cd07032; RNAP_I_II_AC40; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00320; RNApol_arch_Rpo3; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR022842; RNAP_Rpo3/Rpb3/RPAC1.
DR InterPro; IPR033901; RNAPI/III_AC40.
DR InterPro; IPR036643; RNApol_insert_sf.
DR PANTHER; PTHR11800; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR11800:SF13; DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Reference proteome {ECO:0000313|Proteomes:UP000027080};
KW Transcription {ECO:0000256|ARBA:ARBA00023163}.
FT DOMAIN 129..442
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
SQ SEQUENCE 454 AA; 49858 MW; F4352266073811B9 CRC64;
MSTNKSSSYM QVTLNRVDNT ETVSSPHTYA HSGGGSDAQM GKNNVPATTL LDRPQISNLL
ITSAMVGLPI ASLENGKWRK QPNIVAPSSP EVAQVKLEDM VGKGGALGCF ASQGLTSEGF
PRTTRRVEKL SFDMDAVSPP IANLFRRVIT TEVPTLAFDR VLLYENDGVV LDELLSHRLG
LVPVAGPVSL MEYITESSQA SFQSLDPKRV LLFELEAEAE KNVAVTPVYS GQLRWVPLPG
QEGFQQRPND LSATQDDEDN QVFVVHPDIL LTKLGPGQRL KLRAMAVKGI GAVHTKWSPV
SACYYEMKTS IELTEPVVGK AAEELVKMCP KGVFGLVKVP KEDMEDEEED AKLRVGLSKD
SEELSTKPVS VSAIVLDGNK CTISRECLRT DLYPELENKV KIQKNKTSVR FHIESVGQLR
SVQIFRTALT LFAERVRYLA QVIQDTEVTG TVLE
//
