ID W6N5F0_CLOTY Unreviewed; 746 AA.
AC W6N5F0;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=CTDIVETGP_1873 {ECO:0000313|EMBL:CDL91803.1};
OS Clostridium tyrobutyricum DIVETGP.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1408889 {ECO:0000313|EMBL:CDL91803.1, ECO:0000313|Proteomes:UP000019482};
RN [1] {ECO:0000313|EMBL:CDL91803.1, ECO:0000313|Proteomes:UP000019482}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DIVETGP {ECO:0000313|EMBL:CDL91803.1,
RC ECO:0000313|Proteomes:UP000019482};
RA Soggiu A., Piras C., Gaiarsa S., Sassera D., Roncada P., Bendixen E.,
RA Brasca M., Bonizzi L.;
RT "Draft Genome Sequence of Clostridium tyrobutyricum Strain DIVETGP,
RT Isolated from Cow's Milk for Grana Padano Production.";
RL Genome Announc. 3:213-215(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDL91803.1}.
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DR EMBL; CBXI010000032; CDL91803.1; -; Genomic_DNA.
DR RefSeq; WP_017895376.1; NZ_CBXI010000032.1.
DR AlphaFoldDB; W6N5F0; -.
DR GeneID; 29418689; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000019482; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000019482}.
FT DOMAIN 5..285
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 286..561
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 746 AA; 85802 MW; BDE24F6CCF2B34DA CRC64;
MNLRNLLNKE QYEAVTTIEG PLLILAGAGS GKTRVLTYRI AHMIQDLNIY PSKILAITFT
NKAAGEMKDR VKALVGNEAD NMWISTFHSS CVRILRREID KLGYNKNFAI YDSYDQKVLI
KQCMEELDID EKYITGRELI NKISSQKDNL ISAERYKMEN ESNLRLNKVA DVYMLYQKKL
KNNNALDFDD LIYKTVQLFK ENSDVLEFYQ RKFQYIMIDE YQDTNKSQYE LVELLAKSHK
NICVVGDDDQ CIYEWRGADI RNILDFEKDY PNTKVIKLEQ NYRSKANILK AANDIISNNS
ERKTKVLRTE NDNGDKIKLY RAYSDIDEGM YVSSQIKKIC REQNKHYRDF AILYRTNAQS
RVFEDVFMKS NIPYRIVGGL KFYDRKEIKD IMSYLKFINN PLDDVSLRRI INEPKRSIGN
TTVDKVQNFA ASMDECMYSV LLDIDEVLSL PKRSVSSIKK FVSLINSFIR SRDKISVSSL
IKEVIETTGY IDKLKDLKDA ESVSRIENLK ELVSAAVEFE STSEDKSLSA FLEKVTLVSD
IDNFDEDADS VVIMTVHSAK GLEFPVVFMV GMENGIFPGS QSLENSAEME ESRRLCYVGI
TRAKERLYMT SAESRRVFGR MVSYSQSEFL SEISSSLKEY DNINNNITTF GNRSNFNDNK
SWMPRMTKKT SFEVKRDVTI KNKSEEIVNT LKAENVKAGM KVKHNKFGIG TIVSVSKNGS
DIKLTIAFDK NGIKNLMYGV ARLEAV
//
