ID W6RUF1_9CLOT Unreviewed; 527 AA.
AC W6RUF1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0008006|Google:ProtNLM};
GN ORFNames=CM240_0753 {ECO:0000313|EMBL:CDM67918.1};
OS Clostridium bornimense.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1216932 {ECO:0000313|EMBL:CDM67918.1, ECO:0000313|Proteomes:UP000019426};
RN [1] {ECO:0000313|EMBL:CDM67918.1, ECO:0000313|Proteomes:UP000019426}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M2/40 {ECO:0000313|Proteomes:UP000019426};
RA Wibberg D., Puehler A., Schlueter A.;
RT "Complete genome sequence of Clostridum sp. M2/40.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
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DR EMBL; HG917868; CDM67918.1; -; Genomic_DNA.
DR RefSeq; WP_044036586.1; NZ_HG917868.1.
DR AlphaFoldDB; W6RUF1; -.
DR STRING; 1216932.CM240_0753; -.
DR KEGG; clt:CM240_0753; -.
DR PATRIC; fig|1216932.3.peg.739; -.
DR eggNOG; COG1543; Bacteria.
DR HOGENOM; CLU_008192_1_0_9; -.
DR OrthoDB; 9803279at2; -.
DR Proteomes; UP000019426; Chromosome 1.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Reference proteome {ECO:0000313|Proteomes:UP000019426}.
FT DOMAIN 7..335
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 425..524
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 189
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 350
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 258
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 404
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 464
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 527 AA; 61876 MW; E2E0123284644A4B CRC64;
MKKGYVALVL HSHLPFVRHP DINDALEERW LFEVMSECYM PLLKVYENLL DEGIDFKVTM
SITPPLMEML EDEYLNERYF KHLRKLIELT QKEIVRTKND EKMNAVAYFY NERFIELLEI
YNKYDRRILN GFRRFSESGN LEIITCSATH ALLPLLEINE ETVKAQLTTG VENYYEHMGK
MPNGIWLPEC AYTYKLESYL RDLGIKYFIC ESKGIQYGSP RPRYGTAAPV VTPNGVVAFG
RDEESSHQVW SSFAGYPGDV DYREFYRDIG FELPKDYIGP YINAGGIRID TGIKYHRITG
KTENKDIYNR EWAMNKVESH ANHFTHSKND QIEELSRNMD VAPILTCPYD TELFGHWWFE
GPEFIEKFMR KSCEDWTNYQ LITPWEYINK YPSIQCSTPS PSSWGADGDY SVWLNEGNDW
IYKELHQCEV AMTRLANTFD RPSDIERRAL NQAARELMLA ESSDWPFIIT TNTTVKYAVN
RINSHINKFS KLYDDLTKKA IEEKSLNNME KIDNIFKNID YTLYKSK
//
