ID W7J5E4_9PSEU Unreviewed; 1681 AA.
AC W7J5E4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:EWC64201.1};
GN ORFNames=UO65_0527 {ECO:0000313|EMBL:EWC64201.1};
OS Actinokineospora spheciospongiae.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Actinokineospora.
OX NCBI_TaxID=909613 {ECO:0000313|EMBL:EWC64201.1, ECO:0000313|Proteomes:UP000019277};
RN [1] {ECO:0000313|EMBL:EWC64201.1, ECO:0000313|Proteomes:UP000019277}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EG49 {ECO:0000313|EMBL:EWC64201.1,
RC ECO:0000313|Proteomes:UP000019277};
RX PubMed=24604655;
RA Harjes J., Ryu T., Abdelmohsen U.R., Moitinho-Silva L., Horn H., Ravasi T.,
RA Hentschel U.;
RT "Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated
RT Bacterium Actinokineospora sp. Strain EG49.";
RL Genome Announc. 2:e00160-14(2014).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWC64201.1}.
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DR EMBL; AYXG01000020; EWC64201.1; -; Genomic_DNA.
DR RefSeq; WP_052020540.1; NZ_AYXG01000020.1.
DR STRING; 909613.UO65_0527; -.
DR PATRIC; fig|909613.9.peg.542; -.
DR eggNOG; COG1020; Bacteria.
DR OrthoDB; 2378856at2; -.
DR Proteomes; UP000019277; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 2.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.40.50.980; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000019277}.
FT DOMAIN 495..570
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1561..1636
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 1681 AA; 179036 MW; 1EC1ADAC1711AF0A CRC64;
MVLDDFHTQV TEAPDALAVQ DGDLLLTYRD LAAHASALAT RLTDRGVGPE SVVAVYANRS
AELVVAELAT LLAGAAFLPL DPAHPAARIR QLLELSGAAA VLSTEALVSG GAPLGDDVLV
VDLTGEPGGA AFPAPPTPDA AALAYVIYTS GSTGRPKGVA VTHGGLANLM RWHHRTYGTR
RTDRSTLLAS PGFDVSIQDI WFTLTAGAAL VIPPAEVRTS PPELATWLAD EQITVTFLPT
PLAEAVLDET WPAHTALRFL HTGGSAMQRG VPDGLPFTVV NLYGPTEATV KVTACEVLPG
GPVPPPIGVP VDGVRCHVLD GLDPVPDGEA GEMCLAGDCV ARGYLGDPAA TAAAFVPDVT
APGQRMYRTG DKVRRGPDGV FEYIGRYDDQ AKIRGFRIEP GEIATVLKGH PAVHDAFVVV
EHSGRPDARL VAYVVSAVAP AELVEFVAAR LPAYMVPAAT VALPALPLTP NGKVDRHALP
APDRAAAGLD DAAVSPRTPT EVAVAGIVAE LLGTAEVGVH DDFFALGGNS LLVGRLAVPL
ATELNAAVSV AELFNARTVA SLAAIVDERV AAAGGGEPVS PLIAPIPPPL RRVPRDRPIP
LSLPQERVFF FEQLSPGNLA YNFQATVSLR GKVDVGALRS ALDEIVRRHE ILRTAYVAVD
GVGYQRPVAE AKASLKVLDV PAADADEVVA AHLREPFDLG NPPLARWVLL RHEDDQNTFL
HVEHHFVHDG WSLSVFLSEL RVLYPAFVAG LPSPLPELPV QYADYAFWQR DWMRGEVLRA
HVEHWTARLA GAPDTLELPA DRPRPPVISF RGRAPRILFP TELSRALRAF SRQHRVSLFA
TMYAGFAALL HRYTGQRDML VGTGAANRNV PELEPLLGML VNTLVLRTEV APDQRFSDLL
EQVRQSVAET LAWSDTPVDA VIDAIEPTRD PSRTPLFQVM FSFHDSAVPD IDFGGLTGRV
TERSNRSAKS DLNVIVIPRA EQRLGRAASP EDDDLAMIWE HSSDLFDEDT MTRMVTHYLT
LLADAVARPD TRVGALRLLP DAEADQLDTW SRGPAVAGTE PVVELIARQA RTRPDAVAVS
WSEGQLTYGD LWRRAGGLAD ELREAGIAAE EPVAVCADRS VELVVGELAV LLAGGCYLPV
DPSYPAERIT FMLRDAGVRK VLATLQTQLQ LPAPDSSFRV FDLGAPVADG PVDAGDPVAP
DRLAYLIYTS GSTGRPKGVA LSHRGLANLV GWHVGEYGLG PADRTALFAS PGFDASTWEI
WPTLAAGATL CVVPPKLRAA PAELVRWIGD ERITTTFLPT PVAAAVLTEP WPADTALRAL
LTGGDALPAA PPEGLPFRLF NHYGPTENTV VATAGPVPPG PAGPRPPIGR PIAGVDAHVL
DAEGRRVPTG VRGELHLGGT AVARGYVDDP ELTAARFPAD PSAAHPGARL YRTGDLVRWL
PDGQLEFLGR ADEQVKIRGF RIEPEEVAAT LREHPQVRDA VVIARAPEAG AETRLIAYAQ
SGTAAAEDLP GFLRERLPAY MVPDEVVVLP TLPLTEHGKV DHGALAALAP PAPDVPEAEQ
LPRTATEERV AQLASALLRG RRVGRDDDFF RVGGHSLLAA RLVAQVNDAF GVAVPIALFL
QRPTVANLAD AVAVATAGPA TGPIRPSSRR AAARLLSELD QLSDERVESL LRDAEGTEVQ
Q
//
