ID W7JXS1_PLAFA Unreviewed; 1136 AA.
AC W7JXS1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=tRNA(Phe) 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4)]-methyltransferase {ECO:0000256|ARBA:ARBA00012750};
DE EC=2.1.1.282 {ECO:0000256|ARBA:ARBA00012750};
GN ORFNames=C923_02962 {ECO:0000313|EMBL:EWC76381.1};
OS Plasmodium falciparum UGT5.1.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=1237627 {ECO:0000313|EMBL:EWC76381.1, ECO:0000313|Proteomes:UP000030697};
RN [1] {ECO:0000313|EMBL:EWC76381.1, ECO:0000313|Proteomes:UP000030697}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UGT5.1 {ECO:0000313|EMBL:EWC76381.1,
RC ECO:0000313|Proteomes:UP000030697};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Cheeseman I., Volkman S., Adams J., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Plasmodium falciparum UGT5.1.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000256|ARBA:ARBA00001168};
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DR EMBL; KE124582; EWC76381.1; -; Genomic_DNA.
DR AlphaFoldDB; W7JXS1; -.
DR EnsemblProtists; EWC76381; EWC76381; C923_02962.
DR Proteomes; UP000030697; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 2.
DR Gene3D; 3.30.1960.10; tRNA wybutosine-synthesizing-like; 2.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR PANTHER; PTHR46093; ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5; 1.
DR PANTHER; PTHR46093:SF17; MULTIPLE EGF-LIKE-DOMAINS 8; 1.
DR Pfam; PF13418; Kelch_4; 1.
DR Pfam; PF13964; Kelch_6; 1.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF50965; Galactose oxidase, central domain; 1.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF111278; SSo0622-like; 2.
PE 4: Predicted;
KW Kelch repeat {ECO:0000256|ARBA:ARBA00022441};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000030697};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 80..440
FT /note="tRNA wybutosine-synthesizing protein"
FT /evidence="ECO:0000259|Pfam:PF02676"
FT REGION 724..744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1059..1082
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..744
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1136 AA; 135046 MW; 09125523C80B11E4 CRC64;
MDTFYKKKRN VLTLINDEQD LYIYKDIYNN CENDIYSLYL NIINQYKNCL DGEKKLFLNK
CIKNGNVKYI EKVVKQIIEK KNVYNDIDDK SIKKSIDLLI YPCVYEINKN EFYYTTSCCS
GRIVIFKEVH INNKNDNQKK IFKNSTTSQE KKEENISHHL SNINLLNVHE KDELFLSHFK
KNFILQNNEE KYTYCSDHDI SSFFDVSKKK TNKWEDIYEE KNLKENILYI NKEHKKKKVR
KKKKIHKKNV HILYSSHSHQ HLKYDIKVIK DILKKELFQN YFDVNQRNDY KNISFDFMGT
KKYSLQNEVN LNSSIITMSD KEGMKKKEKN NKVINILNNQ NKRNIFIKFE PFIIHVKCTT
FLSALKLLKI AQYAGLKQSG ILNFNKHVTV AIRGSMRLEH LLGDIHPTLQ QTNLMEIIHI
CNNKLSKNLS QLVHFYKCFK QFKEHEHTYQ FVSINREMLS SLENNLSSNT KQKKKSNKKN
TLHVKDNLQD NKKIAHHMKK KKEINHLYLT CSGKKNIPNG NRSIAEQDKT ECKLKDEIIY
ENTENHINII KKEKNIIDLN KYNIQLNEEG YIINENKNDK FIGWKILGNN NMNVQNFFVW
GHDMFMQENK IYMFGGFSKG VRNNKLKIYD IINKKHFIYD TELPSLVFHN FVQLDDKFAF
IFGGRQNPKN CTNMVWVYNI KENFWIKARR TSTLVRKNKN VLFLEKMEKN MEVKMDRMKY
HMGKKYNNDD NNSDNNNDNN NDNNNNNFLC NDENIFYFNN EEEPCPRYRH ASVFVRRYIK
KSKSIYIFYT YGGVNEKNEI LNDIWEGKII LNLEDKGIAH IEWNKKNCSQ KTEACRINNH
SMIYNKKKNF IYIVGGYQDN DKDNYTQYNE YNNNNNNNKD NSVNSDDMIG MPNLSSTVVC
KKMEYLYTYD IKKDNFFYTK CLGDDNMELK AYPSDRFSHS TCLINHNFFM LVGGINIHRT
LNDVWLFHIK TNKWNYLGTF TFQNMYVRSK VVSENNCVYI IGGGCTVFTF GSFFDVPIWS
NFSGIMKSIQ HKELKNVLIF SEDREVKEGQ ATYNNEIKKN CGNNNDNNNN NNNNNNNNNN
NNNKCSNNYN ILVDKVARNE HLEKYNLCHN MLIEHKDVIK KEILVFEKKK KKKKKR
//
