ID W7K965_PLAFO Unreviewed; 2845 AA.
AC W7K965;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=proton-translocating NAD(P)(+) transhydrogenase {ECO:0000256|ARBA:ARBA00012943};
DE EC=7.1.1.1 {ECO:0000256|ARBA:ARBA00012943};
GN ORFNames=PFNF54_05062 {ECO:0000313|EMBL:EWC86332.1};
OS Plasmodium falciparum (isolate NF54).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=5843 {ECO:0000313|EMBL:EWC86332.1, ECO:0000313|Proteomes:UP000030673};
RN [1] {ECO:0000313|EMBL:EWC86332.1, ECO:0000313|Proteomes:UP000030673}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NF54 {ECO:0000313|EMBL:EWC86332.1,
RC ECO:0000313|Proteomes:UP000030673};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Cheeseman I., Volkman S., Adams J., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Plasmodium falciparum NF54.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+);
CC Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349; EC=7.1.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000006};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; KE123877; EWC86332.1; -; Genomic_DNA.
DR EnsemblProtists; EWC86332; EWC86332; PFNF54_05062.
DR Proteomes; UP000030673; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR CDD; cd05304; Rubrum_tdh; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR008143; Ala_DH/PNT_CS2.
DR InterPro; IPR007886; AlaDH/PNT_N.
DR InterPro; IPR007698; AlaDH/PNT_NAD(H)-bd.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR024605; NADP_transhyd_a_C.
DR InterPro; IPR034300; PNTB-like.
DR PANTHER; PTHR10160; NAD(P) TRANSHYDROGENASE; 1.
DR PANTHER; PTHR10160:SF19; PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE; 1.
DR Pfam; PF01262; AlaDh_PNT_C; 1.
DR Pfam; PF05222; AlaDh_PNT_N; 1.
DR Pfam; PF02233; PNTB; 1.
DR Pfam; PF12769; PNTB_4TM; 1.
DR SMART; SM01002; AlaDh_PNT_C; 1.
DR SMART; SM01003; AlaDh_PNT_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52283; Formate/glycerate dehydrogenase catalytic domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00837; ALADH_PNT_2; 1.
PE 4: Predicted;
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW NAD {ECO:0000256|ARBA:ARBA00023027}; NADP {ECO:0000256|ARBA:ARBA00022857};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000030673};
KW Translocase {ECO:0000256|ARBA:ARBA00022967};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1764..1783
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1795..1813
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1819..1839
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1846..1864
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1884..1909
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1921..1938
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2000..2019
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2734..2755
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2761..2780
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2816..2835
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 2324..2450
FT /note="Alanine dehydrogenase/pyridine nucleotide
FT transhydrogenase N-terminal"
FT /evidence="ECO:0000259|SMART:SM01003"
FT DOMAIN 2459..2630
FT /note="Alanine dehydrogenase/pyridine nucleotide
FT transhydrogenase NAD(H)-binding"
FT /evidence="ECO:0000259|SMART:SM01002"
FT REGION 2259..2279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2845 AA; 332756 MW; 3F939EFD89C60CAB CRC64;
MLPRKRVFIH LYNNICGFGI IKEKSFYHKK NEDLYKYNQA YAYKTKKKGN ITKAIEINKE
ILKESNKNIL DLIKIIEENK EDLNIINYVT FFHRVMQIIN KNKEIYIKYN IYINKTIEQN
VKIIFDYLKD TNNILNNINN YNKRLFSSFI WSYSKYISFL NKKNKYFSID NKHKLLLNNK
FTNGSFHNII NYNIHKDTIL PSCDIIKRYE DINISLIQPC NISNSNINHI YQVNKSTNHK
IYNSHIIYKY SIFNKSNIDL LFSYANQYLP LLNPSRYVIV FWSLSKLNKD NKVLHQKYYK
KSVSLIPLLK YKELIILLYS YSYVNFRNFY FYLNIKNYIL KHKIYKQIIS ERNYESLVNM
LLSFINQNIL FLDLYENIVK YIIFKKRYTF INLLNNQELI TLTYCLCKFP IILIDQAQPN
NINIEKKYII NNNIKNIKNN DALYTHQIIF LYEYLKNVII KRCNMIFKEQ LDINYSIFYK
SIINNNNNNN IYTLENLILI IWSLSLKHIY SAKLFLYTFI KLNYMMQNEQ LIYNHYNILT
NFYLSFLSFI LEDHIFINLY FNKENHHSLN ILYQYIDMFL DNFKTCKETI NMNKRKYDVD
ISNMHKNIYQ IINNIKFPNK INTILEYKNV HNISIDILLY KPNYVLQQKK IFILNIIKDK
YDTYNHCMYN NNSGTNTSYE NLKINNILIT MWNKNDNMIK SKEKGQINIY KYFSSIKNCS
KNNIKGTKKQ QKIKMNKNRK IINSNNIFNV KYIKSYFCTN VVKLSNIEKK NNNNNDNLKN
NKENNLFTFI EKIKNFHLKN NKDQLSDTYK ELHNILDIHI VHMKQSDIIS FFYKFSSILT
IFYTINFMEH INELIKTFYY FHNFTFLFLK ETNIRINLFI DICWIYFRYM KFLTYIKRER
NMEKKCIIIN NNNNNNNIYI YNNDQSNIQN VQKIKLYNNS LTYQNCDNNE SNVLFDQLNV
VDNIFNIVKK KILEEDNMIK GISSKNMSFL ISIFIYLNHS EHIRQYLEKN NFHETVFTIK
DILFILTALS KCNNNNYNNI CMWDIQHFFV NKFIDNIKNC QVQDLVDFTN LCAELKVNQE
HISSNIKNKI MSYIYCEANK EKKNIEKKKS ILNHTTIMNI NEGNNKSIMF IELFDEKQLA
FFVRNCYRMH CFDKSFFDLL CDIILKKYCT FHINNLCIAL LCFSRIYCLH KATKFIHKTR
DIKRKEFINI NTNKNTNINT NINTNINTNK NTNKKGDDYL EKNISMCRYD IPLSLKKLIK
YTEKKLLSNT NIDFYNLCYF MESITLLKIQ NKKLYQLALN ETLNNIRQKI YNEQDTKLLG
KILWCLSYYH KTEFIFSFYL IHFMLQNMIY KHIIPENFIS IYLYFIQSRV YNPILFHNIA
QVILSKITLN DYFTHNKNKH ANITNIKLNV ISELYATMSW AYAFVYNETL NKEETYILNK
KEKNEVTNYP LKKREKKISY TENINEDHIL FKTKSYNDDD IMYNDLNFIQ KIYSYIFNEI
KILEKNNNLS FLLLVRFLWG ISLTNLISEP IINFINLYNW NDVNTKEQND MHLHMLFTFW
LRIKYDHSNL HLSNSFIQLK DEIFLLLQKR EFKRNMNKND HISDFHVQIC QILDDLNIRY
HNEYITKDLL SVDIKLERKC CEQKLAIEID GPSHHFLVLN EMQKADPQRI KKNIFASIIL
CLLLFLSSNC YGKSNLRNYI NGFNEFIEDD KNSNFEIKLP VINFPVSSND ITNDYKNEEK
ASRDNLLNNE EIEPSTYYSV LPSLLNAVYL FSSICFILCL TGLNAHRTSK RGNILGFIGI
VAAIMITFSQ VGFGFRYKLF LLIVIPAITI GIYIAHNVSM VQMPQLVALF HSFVGLAALF
VGFSKYHSES FENYEISTIH LLELYVGTFI ASIAFIGSLV AAGKLSGILD SKSLKLQIKK
IINILCIVLI IILGYYFVTL KLLYLKSICL YISLIIDSFL GFHLIASIGA ADMPVIISVL
NSYSGFATAI SGFLLHNNLL IISGALIGSS GAILSYIMCI GMNRDIFNII LGGWDDYENM
GESIYDQNFI EKKNKQTINS TTNKYVAENL INAKNIIIVP GYGTALSKCQ RELAEICSIL
TSRNINVTFA IHPVAGRMPG HLNVLLAEAN IPYNIVKEMN EINPIISEAD IVLVVGANDI
VNPSSLDPSS KIYGMPVIEV WKSKQVIVFK RTLNTGYSAI DNPLFYFSNT FLLFGDAKHT
TNQILTILND YVNNKYPDIS DQDRHINHDK TQFRYSYSLT DSSHSNDDST SKEQNYPKPR
RVIGLIKDDN VQGGNDLLIE HIQSKVENAP NIKDQKRDVN LSIVPISPKF IPKLRLMGFR
ILVERDIGTN ILMQNDEYTK YGAEVVSRNV ILQQSNIILK VDPPTVNFIE EIQNNTILIS
YLWPSINYHL LDKMIQDEEK HNITYLAIDE VPRSTRAQKL DVRSSMSNLQ GYRAVLEAFF
ILPRFSKSSI TAAGKINPAK VFVIGAGVAG LQAIITAKSL GAIVYSHDSR LATEEEVKSC
GGIFIRIPTS ERGDILNMST DMNNEEYIKV QSNLFKKIIK KCDILICSAS IPGKTSPKLV
TTEMIKLMKP GSVAVDLSTE FGDKKNNWGG NIECSQSNEN ILINGVHVLG RDKIERNMPM
QASDLFSMNM INLLEEMGGG VHFNIDMNND IIKSLVVIKD GNILYSPDKS VEKLIKSESV
FINEKNQLIE PISEQKIKYP TGLRLTEKFI ESDTFFYISL LFVIILTFLA ATYLSQSDLQ
SLFLFTLSTI VGYYCVWSVT PALHTPLMSM TNALSGVIII GSMIEYGNGY KDLSSILSML
ATFLSSVNLS GGFYVTKRML DMFFI
//
