ID W7KT44_9CREN Unreviewed; 428 AA.
AC W7KT44;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=Hydrogenase (NiFe) small subunit HydA {ECO:0000313|EMBL:EWG06366.1};
DE Flags: Fragment;
GN ORFNames=ASUL_09924 {ECO:0000313|EMBL:EWG06366.1};
OS Candidatus Aramenus sulfurataquae.
OC Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Aramenus.
OX NCBI_TaxID=1326980 {ECO:0000313|EMBL:EWG06366.1, ECO:0000313|Proteomes:UP000054284};
RN [1] {ECO:0000313|EMBL:EWG06366.1, ECO:0000313|Proteomes:UP000054284}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AZ1-illumnia {ECO:0000313|EMBL:EWG06366.1};
RX PubMed=24604657;
RA Servin-Garciduenas L.E., Martinez-Romero E.;
RT "Draft Genome Sequence of the Sulfolobales Archaeon AZ1, Obtained through
RT Metagenomic Analysis of a Mexican Hot Spring.";
RL Genome Announc. 2:e00164-14(2014).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit
CC family. {ECO:0000256|ARBA:ARBA00006605}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWG06366.1}.
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DR EMBL; ASRH01000035; EWG06366.1; -; Genomic_DNA.
DR AlphaFoldDB; W7KT44; -.
DR PATRIC; fig|1326980.6.peg.1979; -.
DR Proteomes; UP000054284; Unassembled WGS sequence.
DR GO; GO:0009375; C:ferredoxin hydrogenase complex; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 4.10.480.10; Cytochrome-c3 hydrogenase, C-terminal domain; 1.
DR Gene3D; 3.40.50.700; NADH:ubiquinone oxidoreductase-like, 20kDa subunit; 1.
DR InterPro; IPR027394; Cytochrome-c3_hydrogenase_C.
DR InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR InterPro; IPR037148; NiFe-Hase_small_C_sf.
DR InterPro; IPR037024; NiFe_Hase_small_N_sf.
DR InterPro; IPR001821; NiFe_hydrogenase_ssu.
DR PANTHER; PTHR30013:SF5; HYDROGENASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR30013; NIFE / NIFESE HYDROGENASE SMALL SUBUNIT FAMILY MEMBER; 1.
DR Pfam; PF14720; NiFe_hyd_SSU_C; 1.
DR Pfam; PF01058; Oxidored_q6; 1.
DR PIRSF; PIRSF000310; NiFe_hyd_ssu; 2.
DR SUPFAM; SSF56770; HydA/Nqo6-like; 1.
PE 3: Inferred from homology;
KW 3Fe-4S {ECO:0000256|PIRSR:PIRSR000310-1};
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRSR:PIRSR000310-1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR000310-1};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR000310-
KW 1}; Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR000310-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000054284};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 396..415
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 61..280
FT /note="NADH:ubiquinone oxidoreductase-like 20kDa subunit"
FT /evidence="ECO:0000259|Pfam:PF01058"
FT DOMAIN 304..384
FT /note="Cytochrome-c3 hydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14720"
FT BINDING 61
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 174
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 267
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 309
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 312
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 334
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 340
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 368
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 371
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EWG06366.1"
SQ SEQUENCE 428 AA; 45880 MW; CE2757DFE23CDD03 CRC64;
PYSFLPKRFH MSLKISRRDF LKLALTATML AAPEWDKAIG KAVSMIKNGD VNIVWFEAQA
CEGNTTALIQ ATDPTVTQVL FGASPLVGPG SVKISFWPSL MPQQGEQAID VLNDIIAGNH
NPYVLVLEGS FPDENTAQQY NSGGFWGMLG PKTLNEWVAE LLPNAVAVLA VGNCASYGGL
VADKVYQPPP KFITPSWSPS PTGAVGFFDD PLRGYKGLLS RFYEDNYLNA SAGAAPFYTF
VKDPNSYLDI TPSPSASVKP AIAVPGCPAN GNGIMRTLAN LVLWAGGLAP LPELDQYWRP
MYFFQYTVHE QCPRAAWYAA GEFRTQPGEP NAACLFEVGC KGPVSNCPWN KYGWVGGIGG
PTRTGAVCIG CTMPGFTDLY EPFYKPLQVP TPSSTLTTAG LIGAGVVIGA AAGLAEKKMV
RKGGMRSK
//
