ID W7Q5V3_9GAMM Unreviewed; 871 AA.
AC W7Q5V3;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN ORFNames=Q427_19855 {ECO:0000313|EMBL:EWH00398.1};
OS Halomonas sp. BC04.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=1403540 {ECO:0000313|EMBL:EWH00398.1, ECO:0000313|Proteomes:UP000019279};
RN [1] {ECO:0000313|EMBL:EWH00398.1, ECO:0000313|Proteomes:UP000019279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BC04 {ECO:0000313|EMBL:EWH00398.1,
RC ECO:0000313|Proteomes:UP000019279};
RA Farooqui S., Greene A.C., Patel B.K.;
RT "Draft Genome Sequence of Halomonas Strain BC04, an Aromatic-degrading
RT moderate Halophilic Bacterium.";
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWH00398.1}.
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DR EMBL; AZQX01000234; EWH00398.1; -; Genomic_DNA.
DR RefSeq; WP_043515814.1; NZ_AZQX01000234.1.
DR AlphaFoldDB; W7Q5V3; -.
DR PATRIC; fig|1403540.3.peg.3717; -.
DR OrthoDB; 9764318at2; -.
DR Proteomes; UP000019279; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000019279}.
FT DOMAIN 66..538
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 661..793
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 871 AA; 95713 MW; CA50EF99C1F20E0A CRC64;
MNTDYRKPLP GTELDYFDVR AAVDAIQPGA YDTLPYTSRV LAEQLVRRCE PELLTDALKQ
LIERRRDLDF PWYPARVVCH DILGQTALVD LAGLRDAIAE QGGDPAKVNP VVPTQLIVDH
SLAVEHAGFD PDAFDKNRVI EDRRNEDRFH FIEWTKTAFE NVDVIPAGNG IMHQINLEKM
SPVVQARDGV AYPDTCVGTD SHTPHIDCLG VIAIGVGGLE AETVMLGRPS MMRLPDIVGV
ELTGKPKPGI TATDIVLAIT EFLRRERVVG AYLEFFGEGA KSLTIGDRAT ISNMTPEYGA
SAAMFYIDEQ TLDYLTLTGR EPEQVALVET YAKQTGLWAD SLVDAQYERV LTFDLSSVER
NLAGPSNPHR RLPTSALHER GIAVNYDKAQ AEEKAGRMPD GAVIIAAITS CTNTSNPRNV
VGAALLAKKA NELGLIRKPW VKSSFAPGSK VARLYLEEAG LLSELEKLGF GIVAYACTTC
NGMSGALDPK IQQEIIDRDL YATAVLSGNR NFDGRIHPYA KQAFLASPAL VVAYAIAGTV
RFDIEKDVLG IDHNGNPVTL KDLWPSDEEI DAIVGEFVKP EQFKQVYIPM FDLDAAERAE
SPLYDWRPQS TYIRRPPYWE GNMAAVRALK GMRPLAILPD NITTDHLSPS NAIMMDSAAG
EYLHKMGLPE EDFNSYATHR GDHLTALRAT LANPKLFNEM VRDESGEVVQ GSLARIEPEG
EVTRMWEAIE TYMERKQPLI VIAGADYGQG SSRDWAAKGV ALAGVEAIVA EGFERIHRTN
LIGMGVMPLQ FREGTTRHTL QLDGTETYDV EGEASPGATL ELVIHRKTGE VDRVPVTCRL
DTAEEVTVYS AGGVLQRFAK DFLESKGEAV S
//
