ID W7TSL4_9STRA Unreviewed; 746 AA.
AC W7TSL4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 22-FEB-2023, entry version 34.
DE SubName: Full=Methylcrotonoyl-carboxylase {ECO:0000313|EMBL:EWM23339.1};
GN ORFNames=Naga_100053g29 {ECO:0000313|EMBL:EWM23339.1};
OS Nannochloropsis gaditana.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae;
OC Eustigmatales; Monodopsidaceae; Nannochloropsis.
OX NCBI_TaxID=72520 {ECO:0000313|EMBL:EWM23339.1, ECO:0000313|Proteomes:UP000019335};
RN [1] {ECO:0000313|EMBL:EWM23339.1, ECO:0000313|Proteomes:UP000019335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-31 {ECO:0000313|EMBL:EWM23339.1,
RC ECO:0000313|Proteomes:UP000019335};
RX PubMed=23966634; DOI=10.1093/mp/sst120;
RA Corteggiani Carpinelli E., Telatin A., Vitulo N., Forcato C., D'Angelo M.,
RA Schiavon R., Vezzi A., Giacometti G.M., Morosinotto T., Valle G.;
RT "Chromosome Scale Genome Assembly and Transcriptome Profiling of
RT Nannochloropsis gaditana in Nitrogen Depletion.";
RL Mol. Plant 7:323-335(2014).
CC -!- COFACTOR:
CC Name=biotin; Xref=ChEBI:CHEBI:57586;
CC Evidence={ECO:0000256|ARBA:ARBA00001953};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWM23339.1}.
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DR EMBL; AZIL01001690; EWM23339.1; -; Genomic_DNA.
DR AlphaFoldDB; W7TSL4; -.
DR EnsemblProtists; EWM23339; EWM23339; Naga_100053g29.
DR OrthoDB; 1129179at2759; -.
DR Proteomes; UP000019335; Chromosome 17.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd06850; biotinyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 1.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR005481; BC-like_N.
DR InterPro; IPR001882; Biotin_BS.
DR InterPro; IPR011764; Biotin_carboxylation_dom.
DR InterPro; IPR005482; Biotin_COase_C.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
DR InterPro; IPR016185; PreATP-grasp_dom_sf.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR18866; CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE; 1.
DR PANTHER; PTHR18866:SF33; METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF02785; Biotin_carb_C; 1.
DR Pfam; PF00289; Biotin_carb_N; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02786; CPSase_L_D2; 1.
DR SMART; SM00878; Biotin_carb_C; 1.
DR SUPFAM; SSF56059; Glutathione synthetase ATP-binding domain-like; 1.
DR SUPFAM; SSF52440; PreATP-grasp domain; 1.
DR SUPFAM; SSF51246; Rudiment single hybrid motif; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS50979; BC; 1.
DR PROSITE; PS00188; BIOTIN; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Biotin {ECO:0000256|ARBA:ARBA00023267};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Reference proteome {ECO:0000313|Proteomes:UP000019335}.
FT DOMAIN 48..497
FT /note="Biotin carboxylation"
FT /evidence="ECO:0000259|PROSITE:PS50979"
FT DOMAIN 167..365
FT /note="ATP-grasp"
FT /evidence="ECO:0000259|PROSITE:PS50975"
FT DOMAIN 670..744
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
SQ SEQUENCE 746 AA; 80769 MW; 00A9DCFFBCF9152B CRC64;
MWNCGRLVKI HISLPWRTSG SVLYSRSLMS HGSTSATPVE GSPPSSKPFT KLLIANRGEI
ACRIIRTARK MGIKTVAVYS DADARSQFVR MADEAHWIGP SPTTESYLVK EKILEVAKRA
GVQALHPGYG FLSENPDFAE MCQSAGVVFV GPPVPALRSM GSKAAAKRIM TEAGVPVTPA
YYGEEQDDAR LLAEAEKVGY PVMIKAVTGG GGKGMRRVMA AADFLPALEA CRREALKSFG
RDRMLIEKYL VDPRHIELQV FGDRHGNAVH LHERDCSVQR RHQKVLEESP APGLGEALRE
EMGRAAVLAA KAVGYTGAGT VEFLLDPASQ RFYFCEMNTR LQVEHCVTEM VTGVDLVEWQ
LRVAAGEPLP VREQADIRAR GHAIEARIYA ENPAKGFLPA TGSLKRLRTP VSEEGAVRVE
TGVVEGDEVS MYYDPMIAKL LTWAPSRPEA LRRMARGLRD YQIAGLPNNL EFLERCVGHP
AFERGGVTTG FLVEYEADVS IPPPSPPSPH LLALAAYALT QQREAPRGST PWLGGAWRGV
GRRRLPVEFV VDGLEESFAL KAGVMDGGVQ EGGGGGRHAW GLRVEMPDGQ AYRVSGSRCE
GERASGAHGD EWEATVEGVT YKYSAWIEDG GMRGGARVQL WLRSGGLVDD PLQRTTFSLR
VPAAALGHEL AGGVMPRILA PMPGKLVKVL VKAGEAVRKG QSLLILEAMK MEHVITAPQD
ATVDSVARAV GDVVQEGIPL VSLKAA
//
