ID W7U0K5_9STRA Unreviewed; 1009 AA.
AC W7U0K5;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 22-FEB-2023, entry version 27.
DE RecName: Full=Fanconi-associated nuclease {ECO:0000256|RuleBase:RU365033};
DE EC=3.1.4.1 {ECO:0000256|RuleBase:RU365033};
GN ORFNames=Naga_100121g2 {ECO:0000313|EMBL:EWM26169.1};
OS Nannochloropsis gaditana.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae;
OC Eustigmatales; Monodopsidaceae; Nannochloropsis.
OX NCBI_TaxID=72520 {ECO:0000313|EMBL:EWM26169.1, ECO:0000313|Proteomes:UP000019335};
RN [1] {ECO:0000313|EMBL:EWM26169.1, ECO:0000313|Proteomes:UP000019335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-31 {ECO:0000313|EMBL:EWM26169.1,
RC ECO:0000313|Proteomes:UP000019335};
RX PubMed=23966634; DOI=10.1093/mp/sst120;
RA Corteggiani Carpinelli E., Telatin A., Vitulo N., Forcato C., D'Angelo M.,
RA Schiavon R., Vezzi A., Giacometti G.M., Morosinotto T., Valle G.;
RT "Chromosome Scale Genome Assembly and Transcriptome Profiling of
RT Nannochloropsis gaditana in Nitrogen Depletion.";
RL Mol. Plant 7:323-335(2014).
CC -!- FUNCTION: Nuclease required for the repair of DNA interstrand cross-
CC links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of
CC DNA and cleaves DNA successively at every third nucleotide, allowing to
CC excise an ICL from one strand through flanking incisions.
CC {ECO:0000256|RuleBase:RU365033}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolytically removes 5'-nucleotides successively from the
CC 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.;
CC EC=3.1.4.1; Evidence={ECO:0000256|ARBA:ARBA00000983,
CC ECO:0000256|RuleBase:RU365033};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365033}.
CC -!- SIMILARITY: Belongs to the FAN1 family. {ECO:0000256|ARBA:ARBA00005533,
CC ECO:0000256|RuleBase:RU365033}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWM26169.1}.
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DR EMBL; AZIL01000701; EWM26169.1; -; Genomic_DNA.
DR AlphaFoldDB; W7U0K5; -.
DR EnsemblProtists; EWM26169; EWM26169; Naga_100121g2.
DR OrthoDB; 38749at2759; -.
DR Proteomes; UP000019335; Chromosome 9.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004528; F:phosphodiesterase I activity; IEA:UniProtKB-EC.
DR GO; GO:0036297; P:interstrand cross-link repair; IEA:InterPro.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR033315; Fan1-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR014883; VRR_NUC.
DR PANTHER; PTHR15749; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR PANTHER; PTHR15749:SF4; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR Pfam; PF08774; VRR_NUC; 1.
DR SMART; SM00990; VRR_NUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU365033};
KW DNA repair {ECO:0000256|RuleBase:RU365033};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU365033};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365033};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|RuleBase:RU365033};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365033};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU365033};
KW Nucleus {ECO:0000256|RuleBase:RU365033};
KW Reference proteome {ECO:0000313|Proteomes:UP000019335}.
FT DOMAIN 874..994
FT /note="VRR-NUC"
FT /evidence="ECO:0000259|SMART:SM00990"
FT REGION 450..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 811..834
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..495
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 811..825
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1009 AA; 110840 MW; 05DB1A3C6B5453FA CRC64;
MPCDGRSKTE KREQEEDDEV EIIEVLTVRS KRVKSSTLYG YGFGSSTLNS LHPTKTLPSL
PPLPPVPAWG RLIDEQSTAK VALQRFLGFS PPLLRHAYTA CYRGVFNDRF NAVPGMGIAS
IFGRYGSGVR SILNVSGCST FSSRSVEHAE GLVRDQGRSD GQAILNPEVI QDVEGEEDDD
VSSLPNGNAQ GAKFWNKSHR NFNFILNWAL ECSEVLKSCR QDVQDLQKLR VGLSPEAATL
LWQCFHSPRA ICRVHVQREG KKTIVKGKSS DRNDNGALQE LVKQQVVRDV TGDQGALLMH
LYDVFAALRA DELARMTLCL FGSAAAATSA VSSSLPCSWR GRRNASREAS LKSLSTFLER
CQKEGSPERV RRKLGHDLVK WLAKETQAPL QVKGEQQQQQ SFRLVCLAPR LAKLLQRLFA
LFFMTNGYDA EDAVVLWWND LETLRFASRG DVDGAGQGRS GEGLLPFASR GPSTSTKSSA
DDENQEAEAT IQKAERPPRS ELPVAWPGLG SPEIPCAYRQ FADTTLSLSD AFEAAVASGD
SATAYACLHT VSDLFLPRPM LPAHASGSVV SDPACTSLAA LEVTQVKTGT HRFQLDVPAE
YRGLEELPGI LFKVLARVIG RGIGLLERDR KHAQAVHYLK IILDGSEPLP WSLRGHFHSR
LVLDLVHCNR PGEALAACEH ALADPRVDGE DRLFLQAKCL SLAVAPRRWK KPTFPALQTA
PVLELKMAEV QGWRQKGKSL EDSVLACLLQ REGDGMAGRE GCFPGVREED PQQAGHWRGA
HWENGLMLSL FGILCWDVLW SAQPAHSEKE SILATSSQGA NGGRPDCRTR SSRGTRSTQS
CRYQHAPCDL QHNRGFLKSC PGRVSALEQV LARIQRGESG ALLAENHARY HGTLCLGIDW
TTFSLPVLQT VAEALGSSAL MLLCETLIRD YSLLSHGFPD LLLWRDAGPH GGMPAMVRLV
EVKGPGDSLS WAQKYWIDKL LGVGVRVEVA RVVEGNERGL RPGAQRRKA
//
