ID W8GFA1_9MOLU Unreviewed; 613 AA.
AC W8GFA1;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 10-APR-2019, entry version 31.
DE RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN ECO:0000313|EMBL:AHK22439.1};
GN ORFNames=X271_00333 {ECO:0000313|EMBL:AHK22439.1};
OS Candidatus Hepatoplasma crinochetorum Av.
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae;
OC Candidatus Hepatoplasma.
OX NCBI_TaxID=1427984 {ECO:0000313|EMBL:AHK22439.1};
RN [1] {ECO:0000313|EMBL:AHK22439.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Av {ECO:0000313|EMBL:AHK22439.1};
RX PubMed=24482531; DOI=10.1093/gbe/evu020;
RA Leclercq S., Dittmer J., Bouchon D., Cordaux R.;
RT "Phylogenomics of "Candidatus Hepatoplasma crinochetorum," a Lineage
RT of Mollicutes Associated with Noninsect Arthropods.";
RL Genome Biol. Evol. 6:407-415(2014).
CC -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC molecules used as primers for DNA polymerase during DNA
CC replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC ECO:0000256|SAAS:SAAS00709340}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|SAAS:SAAS00709317};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
CC -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC domain that contains the primase activity, and a C-terminal DnaB-
CC binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC -!- SIMILARITY: Belongs to the DnaG primase family.
CC {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
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DR EMBL; CP006932; AHK22439.1; -; Genomic_DNA.
DR STRING; 1427984.X271_00333; -.
DR EnsemblBacteria; AHK22439; AHK22439; X271_00333.
DR KEGG; hcr:X271_00333; -.
DR PATRIC; fig|1427984.3.peg.321; -.
DR KO; K02316; -.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR Gene3D; 3.90.580.10; -; 1.
DR Gene3D; 3.90.980.10; -; 1.
DR HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR InterPro; IPR013264; DNA_primase_core_N.
DR InterPro; IPR037068; DNA_primase_core_N_sf.
DR InterPro; IPR006295; DNA_primase_DnaG.
DR InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR InterPro; IPR030846; DnaG_bac.
DR InterPro; IPR034151; TOPRIM_DnaG_bac.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR002694; Znf_CHC2.
DR Pfam; PF08275; Toprim_N; 1.
DR Pfam; PF01807; zf-CHC2; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SMART; SM00400; ZnF_CHCC; 1.
DR TIGRFAMs; TIGR01391; dnaG; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993445};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709369};
KW DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709327};
KW Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709338};
KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709339, ECO:0000313|EMBL:AHK22439.1};
KW Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709304};
KW Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709341};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993442, ECO:0000313|EMBL:AHK22439.1};
KW Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709301}.
FT DOMAIN 260 341 Toprim. {ECO:0000259|PROSITE:PS50880}.
FT ZN_FING 41 65 CHC2-type. {ECO:0000256|HAMAP-Rule:
FT MF_00974}.
SQ SEQUENCE 613 AA; 72333 MW; D21288875C4DD585 CRC64;
MTAKKDLNEL IEKITNDLNI EDVISHYISL EKKGNSFVAI CPFHQDSNPS LSISSTKKIF
KCFVCNVGGN AITFVQKYKK VNYLEAIKII SEDFNLNWND YFVKKERQED PKIKEIKNIN
LEALNFYKYN LQIEIEKNKK LKDYLNERKF TEKLIEVFNI GWAGSNDGLK KYLLKKNFKE
QEILRASLIK NNNGNLRDLF FNRLIFPIFN QFNDVLGFSG RVIDQKSPIK YLNSSQNLLF
NKSKIIYNLN NATEEILLKD NVIIVEGFMD VISFYKIGIK NVVASMGTAF NINHIDKLKS
FTKNFILSFD NDQAGIQTTI KTYNILKNHN INLSVINFSD GKDIDEIINN NLNINKKYFE
DNKISFLDFY YQKVIVKEKL ETLTEEKLKE ILLIVLSYND LVKKEIILLK IKEKFGKDFI
NSFIQNLNFK PLVKKEIIIN NKINLKITNI NKNKNFSSLN NTLNKYLGKY YQEEFKFLLY
SLLSSSFFNN LIFENFIFIN KYYKEIFDLF KNNKALLKSN DDKINYLKTL LQKFSSQREE
NIIKSLLENF QENIKNENLL KTIKNINVED FISNLHEKWR IINKEKHKKK FIENINANDD
EEYNYMINKL KIS
//
