ID W9AIP9_9BACI Unreviewed; 390 AA.
AC W9AIP9;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Polyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG {ECO:0000313|EMBL:CDO02546.1};
GN Name=pksG {ECO:0000313|EMBL:CDO02546.1};
GN ORFNames=BN988_01016 {ECO:0000313|EMBL:CDO02546.1}, OPHB3_2252
GN {ECO:0000313|EMBL:GAQ18313.1};
OS Oceanobacillus picturae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX NCBI_TaxID=171693 {ECO:0000313|EMBL:CDO02546.1, ECO:0000313|Proteomes:UP000028863};
RN [1] {ECO:0000313|EMBL:CDO02546.1, ECO:0000313|Proteomes:UP000028863}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S1 {ECO:0000313|EMBL:CDO02546.1,
RC ECO:0000313|Proteomes:UP000028863};
RA Croce O., Lagier J.C., Raoult D.;
RT "Draft genome sequencing of Oceanobacillus picturae strain S1 isolated from
RT human gut.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CDO02546.1, ECO:0000313|Proteomes:UP000028863}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S1 {ECO:0000313|EMBL:CDO02546.1,
RC ECO:0000313|Proteomes:UP000028863};
RA Urmite Genomes U.;
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000052946}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Heshi-B3 {ECO:0000313|Proteomes:UP000052946};
RA Akuzawa S., Nakagawa J., Kanekatsu T., Kanesaki Y., Suzuki T.;
RT "Draft Genome Sequence of Oceanobacillus picturae Heshi-B3 that Was
RT Isolated from Fermented Rice Bran with Aging Salted Mackerel, Which Was
RT Named Heshiko as Traditional Fermented Seafood in Japan.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:GAQ18313.1, ECO:0000313|Proteomes:UP000052946}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Heshi-B3 {ECO:0000313|EMBL:GAQ18313.1,
RC ECO:0000313|Proteomes:UP000052946};
RA Akuzawa S., Nagaoka J., Kanekatsu M., Kanesaki Y., Suzuki T.;
RT "Draft Genome Sequence of Oceanobacillus picturae Heshi-B3, Isolated from
RT Fermented Rice Bran in a Traditional Japanese Seafood Dish.";
RL Genome Announc. 4:e01621-15(2016).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000256|ARBA:ARBA00007061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDO02546.1}.
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DR EMBL; CCAX010000001; CDO02546.1; -; Genomic_DNA.
DR EMBL; BBXV01000026; GAQ18313.1; -; Genomic_DNA.
DR RefSeq; WP_036573675.1; NZ_QWLU01000013.1.
DR AlphaFoldDB; W9AIP9; -.
DR STRING; 171693.BN988_01016; -.
DR eggNOG; COG3425; Bacteria.
DR OrthoDB; 9769523at2; -.
DR Proteomes; UP000028863; Unassembled WGS sequence.
DR Proteomes; UP000052946; Unassembled WGS sequence.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:InterPro.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR CDD; cd00827; init_cond_enzymes; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR011554; HMG_CoA_synthase_prok.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF08540; HMG_CoA_synt_C; 1.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000028863}.
FT DOMAIN 2..165
FT /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01154"
FT DOMAIN 253..350
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT ACT_SITE 79
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 111
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 233
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 148
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 242
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 275
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ SEQUENCE 390 AA; 43241 MW; 24382B197BF8608B CRC64;
MKIGIDKIGF YAPHLYVDMN KLAIARDVEP AKFTIGIGQE EMAFAPITQD TVTLAANAAL
NILDETDKQK IDFVIFGTET GIDSSKASAV YVHDLLGINP DARSIEMKQA CYAATAAIQM
AKGHIALNPE SKVLVLASDI ARYGLRTGGE ATQGAGAVAM LISADPKILA LEEKSAYHTS
DVMDFWRPTY TDKAIVDGKL SNEQYIEFFA KVWEQYKVKT DASFSDFEAI CFHLPYTKMG
QKALKPLLPE TDEVTQNRLL ENNEASKTYN KRIGNIYTGS LYLSLLSLLE TKEDLEEGAR
IGLFSYGSGA VGEFFSGILQ PGFRSALQPA RHREILDSRK EIDVAEYEER FNNTLPTDGS
TLELEKEDDP SSIYLAGIKN HMRQYVNDNR
//
