ID   W9G4B0_9MICO            Unreviewed;       470 AA.
AC   W9G4B0;
DT   14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT   14-MAY-2014, sequence version 1.
DT   27-MAR-2024, entry version 39.
DE   SubName: Full=Malate dehydrogenase {ECO:0000313|EMBL:EWS99617.1};
GN   ORFNames=N865_21835 {ECO:0000313|EMBL:EWS99617.1};
OS   Intrasporangium oryzae NRRL B-24470.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC   Intrasporangium.
OX   NCBI_TaxID=1386089 {ECO:0000313|EMBL:EWS99617.1, ECO:0000313|Proteomes:UP000019489};
RN   [1] {ECO:0000313|EMBL:EWS99617.1, ECO:0000313|Proteomes:UP000019489}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-24470 {ECO:0000313|EMBL:EWS99617.1,
RC   ECO:0000313|Proteomes:UP000019489};
RA   Liu H., Wang G.;
RT   "Intrasporangium oryzae NRRL B-24470.";
RL   Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EWS99617.1}.
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DR   EMBL; AWSA01000087; EWS99617.1; -; Genomic_DNA.
DR   RefSeq; WP_034810215.1; NZ_AWSA01000087.1.
DR   AlphaFoldDB; W9G4B0; -.
DR   STRING; 1386089.N865_21835; -.
DR   PATRIC; fig|1386089.3.peg.4176; -.
DR   eggNOG; COG0281; Bacteria.
DR   OrthoDB; 9805787at2; -.
DR   Proteomes; UP000019489; Unassembled WGS sequence.
DR   GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR   Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR   InterPro; IPR012301; Malic_N_dom.
DR   InterPro; IPR037062; Malic_N_dom_sf.
DR   InterPro; IPR012302; Malic_NAD-bd.
DR   InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR   PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   SMART; SM01274; malic; 1.
DR   SMART; SM00919; Malic_M; 1.
DR   SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000019489}.
FT   DOMAIN          92..225
FT                   /note="Malic enzyme N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM01274"
FT   DOMAIN          237..459
FT                   /note="Malic enzyme NAD-binding"
FT                   /evidence="ECO:0000259|SMART:SM00919"
SQ   SEQUENCE   470 AA;  48584 MW;  78D934889C2A8E70 CRC64;
     MATPSPGYSI TIRVEVPAAA TSTAALTAAV ASAGGALTAL DVAESRTDRL VVDVTCDTSD
     EAHAKLITEA IGRIPGAEVR KVSDQTFLLH LGGKLEVVPK VPLKHRDDLS RAYTPGVARV
     CLAIAAHPED ARRLTIKRNT VAVVTDGSAV LGLGNIGPLA AIPVMEGKAA LFKQFGGVDA
     WPVCLDTQDS DEIVSIVKAI APVYGGINLE DISAPRCFEI EARLREALDI PVFHDDQHGT
     AIVVLAALTN ALRVVGKRLE DVRIVVSGVG AAGHAIIRLL LSQGASDIIG CGRHGAVHEG
     QDGMEPFRLW IAEHTNRSRR EGSLAEVLVG ADVFIGVSAP NLLTGDDIAT MAPDAIVFAL
     ANPVPEVDPL VASEHAAVVA TGRSDAPNQI NNVLVFPGFF RGLLDAGVSG ITDEMLVAAA
     HAIAGVVTDE QLNASYIIPS VFDPAVAAAV AEAVRGAASA EGVHSPAAEA
//