ID W9G783_9MICO Unreviewed; 1257 AA.
AC W9G783;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=N865_21285 {ECO:0000313|EMBL:EWT00683.1};
OS Intrasporangium oryzae NRRL B-24470.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Intrasporangium.
OX NCBI_TaxID=1386089 {ECO:0000313|EMBL:EWT00683.1, ECO:0000313|Proteomes:UP000019489};
RN [1] {ECO:0000313|EMBL:EWT00683.1, ECO:0000313|Proteomes:UP000019489}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-24470 {ECO:0000313|EMBL:EWT00683.1,
RC ECO:0000313|Proteomes:UP000019489};
RA Liu H., Wang G.;
RT "Intrasporangium oryzae NRRL B-24470.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWT00683.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AWSA01000036; EWT00683.1; -; Genomic_DNA.
DR AlphaFoldDB; W9G783; -.
DR STRING; 1386089.N865_21285; -.
DR PATRIC; fig|1386089.3.peg.3044; -.
DR eggNOG; COG5013; Bacteria.
DR Proteomes; UP000019489; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000019489}.
FT DOMAIN 54..118
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
FT REGION 356..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1257 AA; 137954 MW; 57E271F01DA70B07 CRC64;
MGGTPPQIDG SASAALLKAG RFFTRWDESD DGRAVFREGG RAGDVFYRDR WSHDKVVRST
HGVNCTGSCS WKVYVKDGII TWETQQTDYP SVGPDRPEYE PRGCPRGAAF SWYTYSPTRV
RYPYVRGVLL EMYREAKARL GDPVLAWAEI TGDPEKRRRY QRARGKGGLV RASWGEAVEI
AAAAHVHTIK TYGPDRCAGF SPIPAMSMVS HCVGTRFIQL IGGVMTSFYD WYADLPVASP
QVFGDQTDVP ESGDWWDATY LMMWGSNVPV TRTPDAHWMA EVRYRGTKVV TVSPDYADNT
KFADEWMPAQ AGTDAALAMA MGHVILTENF VRRDTPFFSD YVRTYTDLPF LVRLEPADPS
QGSSSATEAY AAEPGSEGSG PVVPGKFLTA ADLVDNGERP PSEEAWKTVL LDEATGEPVV
PNGSMGFRYA ESGKGRWNLD LEGVTPALTL EGEASEAVEV LLPAFVEPDG TGSVLRRGVP
ARRVHGHLVT TVYDLMLAQY GVARDGLPGE WPSGYDDVST PYTPAWQAEV TGVPAEQCIR
IAREFASNSE ESEGRSMIIL GAGVCQWFHG DATYRAILSL LIMTGCMGRN GGGWAHYVGQ
EKCRPITGWI SLANALDWSR PPRTMTGTSY WYMHTGQWRT DGYSADSLAS PLARGHLAGK
HTADTIAQSA RLGWMPFYPQ FGTNPLQVAE DAAAAVAEGN VPNPAAYVAQ ALHEGTLTTA
IEDVDAPENW PRTLVLWRSN LMGSSAKGNE YFLKYLLGTH SNVMGSESPD APRPSEVRWH
DDAPEGKLDL LMSADFRMTS TTLLSDIVFP AATWYEKHDL SSTDMHPFVH AFTPAIDPPW
EAKSDFEMFH LLAQRFSAMA TTHLGVRKDL VSVPLMHDTP GEVAQPGGRV FDWRGSGLPG
TPGVTMPVFT VVERDYTAIA DKLATVGPLA DTLGFTVKNV TYRLEEETAR LAAKNGVMLG
GAGDGRPAID TDEKLAEAIL SFSGTTNGHL AVQGFHTLEE RVGKKLADLA EGAEEKRITF
ADTQVAPVPV ITSPEWSGSE TGGRRYAPFT VNIERLKPFH TLTGRMHFYL DHDWVQDLGE
ALPIFRPPLD MHRLFGEPRL GPDGAKQVTV RYLTPHSKWS IHSEYQDNLL MLSLSRGGPT
VWMSPGDAAS IDVHDNDWVE CLNANGVLVG RAIVSHRMPD GVVYVHHAQE RTIDVPKSEA
TGRRGGIHNS VTRLLVKPTH LVGGYAQLSY TFNYLGPTGN QRDMVSTVRK RSQEVTY
//
