ID W9GA74_9MICO Unreviewed; 351 AA.
AC W9GA74;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=N865_03895 {ECO:0000313|EMBL:EWT03066.1};
OS Intrasporangium oryzae NRRL B-24470.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Intrasporangium.
OX NCBI_TaxID=1386089 {ECO:0000313|EMBL:EWT03066.1, ECO:0000313|Proteomes:UP000019489};
RN [1] {ECO:0000313|EMBL:EWT03066.1, ECO:0000313|Proteomes:UP000019489}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-24470 {ECO:0000313|EMBL:EWT03066.1,
RC ECO:0000313|Proteomes:UP000019489};
RA Liu H., Wang G.;
RT "Intrasporangium oryzae NRRL B-24470.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWT03066.1}.
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DR EMBL; AWSA01000005; EWT03066.1; -; Genomic_DNA.
DR RefSeq; WP_034801613.1; NZ_AWSA01000005.1.
DR AlphaFoldDB; W9GA74; -.
DR STRING; 1386089.N865_03895; -.
DR PATRIC; fig|1386089.3.peg.684; -.
DR eggNOG; COG2070; Bacteria.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000019489; Unassembled WGS sequence.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR CDD; cd04730; NPD_like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001295; Dihydroorotate_DH_CS.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
DR PROSITE; PS00912; DHODEHASE_2; 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000019489}.
FT DOMAIN 8..347
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 351 AA; 35764 MW; 004FE6F48969DC8A CRC64;
MVISSLDVPV VGAPMAGGPS TPELVAAVGD AGGLGFLAGA YLTTEALARD IADVWDATSR
PFGVNLFVPA HANTYAAGPG QLVGSDRARA VAAYRASLAP LAGRLGVELP EPSGEEPGAW
ERALDLVVRE RVPVVSFTFG LPSKAVLGEL RRAGIESVVT VTDVPEATAA IEAGVDALCV
QGPEAGGHRG TLDEAKDPGE HELVGLVRSV RALTDLPVVA AGGIADSARA AEVLAAGATA
AQIGTALLLT PEAGTGLAYR RALVDPDYAG TTVTRAFSGR WARALETEFV REHSVHAPAA
FPEVHHVTAP LRRASAAADD PGLIALWAGT AWRSARAVPA AEVVRSIAGA L
//
