ID W9H4L8_9PROT Unreviewed; 310 AA.
AC W9H4L8;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=isochorismatase {ECO:0000256|ARBA:ARBA00012100};
DE EC=3.3.2.1 {ECO:0000256|ARBA:ARBA00012100};
GN ORFNames=N825_05975 {ECO:0000313|EMBL:EWY39632.1};
OS Skermanella stibiiresistens SB22.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Skermanella.
OX NCBI_TaxID=1385369 {ECO:0000313|EMBL:EWY39632.1, ECO:0000313|Proteomes:UP000019486};
RN [1] {ECO:0000313|EMBL:EWY39632.1, ECO:0000313|Proteomes:UP000019486}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB22 {ECO:0000313|EMBL:EWY39632.1,
RC ECO:0000313|Proteomes:UP000019486};
RA Zhu W., Wang G.;
RT "The genome sequence of Skermanella stibiiresistens.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000128};
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|PIRSR:PIRSR001111-50};
CC Note=Binds 1 phosphopantetheine covalently.
CC {ECO:0000256|PIRSR:PIRSR001111-50};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWY39632.1}.
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DR EMBL; AVFL01000011; EWY39632.1; -; Genomic_DNA.
DR RefSeq; WP_037454079.1; NZ_AVFL01000011.1.
DR AlphaFoldDB; W9H4L8; -.
DR STRING; 1385369.N825_05975; -.
DR PATRIC; fig|1385369.3.peg.3402; -.
DR OrthoDB; 9807387at2; -.
DR Proteomes; UP000019486; Unassembled WGS sequence.
DR GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.50.850; Isochorismatase-like; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR016291; Isochorismatase.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR PANTHER; PTHR43540:SF3; ENTEROBACTIN SYNTHASE COMPONENT B; 1.
DR PANTHER; PTHR43540; PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR Pfam; PF00550; PP-binding; 1.
DR PIRSF; PIRSF001111; Isochorismatase; 1.
DR PRINTS; PR01398; ISCHRISMTASE.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF52499; Isochorismatase-like hydrolases; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Phosphopantetheine {ECO:0000256|PIRSR:PIRSR001111-50};
KW Phosphoprotein {ECO:0000256|PIRSR:PIRSR001111-50};
KW Reference proteome {ECO:0000313|Proteomes:UP000019486}.
FT DOMAIN 228..304
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT MOD_RES 265
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000256|PIRSR:PIRSR001111-50"
SQ SEQUENCE 310 AA; 33728 MW; 009333FBA70AEB6F CRC64;
MAIPTIQSYP MPGPADLPAG KVAWRPDPER AVLLIHDMQE YFLDFFDVSR EPIPELIANL
RRVRERCAAL GIPVVFTAQP AEQTPEQRGL LTDMWGHGLK TRPHRQPVIA ELAPAPGDVV
LVKWRYSAFQ RSDLRQRMLD WDRDQLIIGG VYAHIGCMMT AADAFMGDVK PFLVADALAD
FSAENHATAL TWVAQRCGVV TTTASLLDDL GEPRATVVDP GSLAAGAAHP PAGPADVLAD
VAQALGVPVA DIGLDDNLLD HGIDSIRIMS LVERWRRVGA EVTFVELADS PTVGAWWALL
ATRIGHTRDA
//
