ID W9UPP8_9GAMM Unreviewed; 822 AA.
AC W9UPP8;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=D791_03880 {ECO:0000313|EMBL:EXJ09183.1};
OS Nitrincola nitratireducens.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Oceanospirillaceae; Nitrincola.
OX NCBI_TaxID=1229521 {ECO:0000313|EMBL:EXJ09183.1, ECO:0000313|Proteomes:UP000019464};
RN [1] {ECO:0000313|Proteomes:UP000019464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK23 {ECO:0000313|Proteomes:UP000019464};
RA Singh A., Pinnaka A.K., Vaidya B.;
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EXJ09183.1, ECO:0000313|Proteomes:UP000019464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK23 {ECO:0000313|EMBL:EXJ09183.1,
RC ECO:0000313|Proteomes:UP000019464};
RX PubMed=26481633; DOI=10.1016/j.syapm.2015.09.002;
RA Singh A., Vaidya B., Tanuku N.R., Pinnaka A.K.;
RT "Nitrincola nitratireducens sp. nov. isolated from a haloalkaline crater
RT lake.";
RL Syst. Appl. Microbiol. 38:555-562(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EXJ09183.1}.
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DR EMBL; AONB01000030; EXJ09183.1; -; Genomic_DNA.
DR AlphaFoldDB; W9UPP8; -.
DR STRING; 1229521.D791_03880; -.
DR Proteomes; UP000019464; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR046816; MmeI_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF20473; MmeI_Mtase; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EXJ09183.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EXJ09183.1}.
FT DOMAIN 139..211
FT /note="MmeI-like DNA-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20473"
FT DOMAIN 332..432
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 822 AA; 91587 MW; 277C9ADE8E21DBC0 CRC64;
MFELKLAPNH QLDLEKLLIS LPTEVFTATD SLGWVYQFWQ ANNKERINKS EVKIGARELP
AVTQLFTEPY MVSFLLDNSL GAWWAARRLS EDDFKNAGSE EELRRKAAID GVPLEYLRFV
QGENGTWEPA SGIFDAWPKQ LSQLSTLDPS CGSGHFLVAA FNMLVPLRME LEQLDAQQAA
DAVLRDNIHG LELDQRCVEL AAFALALSAW KYPNAGGYRV LPEIQVACSG LAINAAKDEW
KQLAAHMGGK HNLSIAMDWL HDNFKDAPVL GSLMNPAKSK AAGLVSWQEL SEALDKALAT
ESAQESQTNE QHEAAHENAV VAQGMAKAAK LLASQYQWVV TNVPYLVKGK QVQRLQDYCS
QMYPLSKNDL ATVFLERCLE FCAVNGTVGA VLPQTWLFQK YYESFRKKLL INDRWHLVAR
LGEGGFESSA AAGAFATMVT LSRGGEQQLA RSSGLFKSLE EKQAVRGLDV SQPKTAPEKA
ILIKDGEIRT VLQNQLLENP DSRISIEDIK KTELLLDFAM SVEGLSTGDG ERFVNSFWEI
EIEKNELWDV FQRSPESKSH YSGLSSVLRW ERNDGELSKS KQARLQGYAA WGKSGVLINL
MRSLHAAIYF GNKHDKITAA LVLKDKESKD FSRVQLESMI WAYCSSPDFN IEVRKLNPAL
QPGTGTLVKV PFDIGYWAKV AEDKYPNGLP KPYTDDPTQW IFHGHPCGSV IWDEAEKWTT
DGPLRTDETV LHIAVARLLG YRWPAELDAT MEQADEQREW VQRCEALLPL ADEDGIVCIP
AVRGEALAAD RLINLLAAAY GDAWTPATLN QLLSSVAMPV KP
//
