ID W9VMF8_9GAMM Unreviewed; 970 AA.
AC W9VMF8;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Aminomethyltransferase {ECO:0000313|EMBL:EXJ11705.1};
DE EC=2.1.2.10 {ECO:0000313|EMBL:EXJ11705.1};
GN Name=gcvT_3 {ECO:0000313|EMBL:EXJ11705.1};
GN ORFNames=D791_01478 {ECO:0000313|EMBL:EXJ11705.1};
OS Nitrincola nitratireducens.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Oceanospirillaceae; Nitrincola.
OX NCBI_TaxID=1229521 {ECO:0000313|EMBL:EXJ11705.1, ECO:0000313|Proteomes:UP000019464};
RN [1] {ECO:0000313|Proteomes:UP000019464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK23 {ECO:0000313|Proteomes:UP000019464};
RA Singh A., Pinnaka A.K., Vaidya B.;
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EXJ11705.1, ECO:0000313|Proteomes:UP000019464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK23 {ECO:0000313|EMBL:EXJ11705.1,
RC ECO:0000313|Proteomes:UP000019464};
RX PubMed=26481633; DOI=10.1016/j.syapm.2015.09.002;
RA Singh A., Vaidya B., Tanuku N.R., Pinnaka A.K.;
RT "Nitrincola nitratireducens sp. nov. isolated from a haloalkaline crater
RT lake.";
RL Syst. Appl. Microbiol. 38:555-562(2015).
CC -!- SIMILARITY: Belongs to the GcvT family.
CC {ECO:0000256|ARBA:ARBA00008609}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EXJ11705.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AONB01000005; EXJ11705.1; -; Genomic_DNA.
DR RefSeq; WP_036509502.1; NZ_AONB01000005.1.
DR AlphaFoldDB; W9VMF8; -.
DR STRING; 1229521.D791_01478; -.
DR PATRIC; fig|1229521.3.peg.1493; -.
DR OrthoDB; 5287468at2; -.
DR Proteomes; UP000019464; Unassembled WGS sequence.
DR GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.10.20.440; 2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR042204; 2Fe-2S-bd_N.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR041117; SoxA_A3.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF17806; SO_alpha_A3; 1.
DR SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF103025; Folate-binding domain; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000313|EMBL:EXJ11705.1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Transferase {ECO:0000313|EMBL:EXJ11705.1}.
FT DOMAIN 166..437
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 492..576
FT /note="SoxA A3"
FT /evidence="ECO:0000259|Pfam:PF17806"
FT DOMAIN 587..849
FT /note="Aminomethyltransferase folate-binding"
FT /evidence="ECO:0000259|Pfam:PF01571"
FT DOMAIN 873..955
FT /note="Glycine cleavage T-protein C-terminal barrel"
FT /evidence="ECO:0000259|Pfam:PF08669"
SQ SEQUENCE 970 AA; 107182 MW; 5A5A2F087E796F3C CRC64;
MNYRLEPQPY EWISRDNAIR FTFEGREFQA FEGDSISSGL LANGQSILGR SFKYHRPRGA
LTLANHDVNA LFQTDEKPNV RGDVVALEPG MTLTACNVNG TVIKDKDAKI EYAARFLPVG
FYYKTFHKPK KLFPFWEKII RSKAGLGQVN TQWDAQRQSK SYAFCDLLVI GAGLSGMQSA
LAAADAQLNV VLVDENPFIG GSLDYQLAHE PRVKALRESL KASVSTHPNI RLYTSTLAVG
WYTDHYLPLL TPHGIIKLRS RAVVHATGVM EQPAVFRNND LPGVMMASGI QRLISRYAVK
PGQNALVLTS NDEGYRAALD MCEAGIAVVA VVDMESIAQR GEWAERVLAK GIRVLEKHGI
YEAIGRQSVQ RVIVAPLSGE SCDLAKKDVI DCDLIAMSVG WVPAAQLIYQ AGGKLSYDET
LQQFVPESLP KGIFVCGRLN GAFTVDQRIS DAKAVAQEAV AFLKGQHEPR DLSQYRDTTA
HSHPWPIIEH PKGKNFIDFD EDLQLKDLKN AILEGFDNIE LLKRFSTVGM GPSQGKHSNM
NAIRVLARAQ GLSINATGST TARPFFHPVP ISALAGRRFR PERRSPLHAW HEAHQAKFME
AGVWLRPEYY GASSARLDCI QEEVEAVRSR LGIIDVSTLG KIEVMGPDAA RLLDLAYTMK
MSTLKQGMTR YALMVDDSGV IIDDGIVGRV ASDQFYVTAT TSHADATYKV LSKYVQFCRL
NVRLVNRTGS LAAINLAGPE SRKVLAPLTS ISLSESDFPY LGMREGLLCG YPVRLIRVGF
VGELGYEIHL PHDAALEVWQ RLMAAGQTVG IKPFGVEAQR ILRLEKGHII IGQDTDGLTH
PYEVGMSWAV PLNSKAWFTG KPSLARLKSS CQRQLVGFKL AQGAPKDLVK ECHLVIDNNE
MVGRVTSVAY SPTQQCVIGL VMLDQPYADT SNPIHIRVDD GRLIRGERCR LPFYDPEGIR
QTSAVEEVLL
//
