ID X1WHL7_DANRE Unreviewed; 1112 AA.
AC X1WHL7; A0A8M2BIL1;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 20-JUN-2018, sequence version 3.
DT 27-MAR-2024, entry version 63.
DE SubName: Full=Solute carrier family 12 member 7 isoform X2 {ECO:0000313|RefSeq:XP_005171307.1};
DE SubName: Full=Solute carrier family 12 member 7a {ECO:0000313|Ensembl:ENSDARP00000129730};
GN Name=slc12a7a {ECO:0000313|Ensembl:ENSDARP00000129730,
GN ECO:0000313|RefSeq:XP_005171307.1,
GN ECO:0000313|ZFIN:ZDB-GENE-070912-696};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000129730};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000129730, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000129730};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000313|Ensembl:ENSDARP00000129730}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000129730};
RG Ensembl;
RL Submitted (MAR-2014) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:XP_005171307.1}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_005171307.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(in) + K(+)(in) = chloride(out) + K(+)(out);
CC Xref=Rhea:RHEA:72427, ChEBI:CHEBI:17996, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034453};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR EMBL; CR788285; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_005171307.1; XM_005171250.4.
DR Ensembl; ENSDART00000157041; ENSDARP00000129730; ENSDARG00000073756.
DR Ensembl; ENSDART00000157041.4; ENSDARP00000129730.3; ENSDARG00000073756.7.
DR GeneID; 558214; -.
DR AGR; ZFIN:ZDB-GENE-070912-696; -.
DR CTD; 558214; -.
DR ZFIN; ZDB-GENE-070912-696; slc12a7a.
DR OrthoDB; 5490251at2759; -.
DR Proteomes; UP000000437; Chromosome 2.
DR Bgee; ENSDARG00000073756; Expressed in spleen and 24 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IBA:GO_Central.
DR GO; GO:0006884; P:cell volume homeostasis; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR000076; KCL_cotranspt.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF47; SOLUTE CARRIER FAMILY 12 MEMBER 7; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 2.
DR Pfam; PF03522; SLC12; 3.
DR PRINTS; PR01081; KCLTRNSPORT.
PE 1: Evidence at protein level;
KW Chloride {ECO:0000256|ARBA:ARBA00023214};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Potassium {ECO:0000256|ARBA:ARBA00022958};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538};
KW Proteomics identification {ECO:0007829|PeptideAtlas:X1WHL7};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Symport {ECO:0000256|ARBA:ARBA00022847};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022847}.
FT TRANSMEM 118..139
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 151..174
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 186..208
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 247..266
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 273..296
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 451..477
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 497..515
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 551..569
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 575..596
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 608..624
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 124..291
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 399..690
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 704..821
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 822..959
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 1043..1112
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1112 AA; 123249 MW; 5BF9876A2B76AAD3 CRC64;
MPTNFTVVPV EDTPLSDLDC DGDGLKEEDK EEPETAAGHY PGDETPRETS SLISTDHDDS
TYGKNMALYE EEMDSTPMVS SLLNKLANYT NITQGAVEHE EAESEDGIQR VTVNGPQMGT
FIGVFLPCTQ NILGVILFLR LTWIVGTAGI LEALAVVFMC CACTMLTAIS MSAIATNGVV
PAGGSYYMIS RALGPEIGGA VGLCFYLGTT FAGSMYILGT MEIFLTYIMP SAAVFEANNP
TGMQNNMRIY GTCCLAIMTL VVFVGVKYVN KLSLVFLSCV VLSIMAIYAG VIQSAIKPSS
FTICLLGNRT LQHFGKSGKF DKCMKTEVID NITKPTELWY HFCNGTCDEY FNLNELTEIS
AVPGLLSNAI TENLWSRYGE YRTTVEKENI TSEAALSSEP EVLNSYVVND MATFFTLLVG
IYFPSVTGIM AGSNRSGDLR DPQRSIPTGT IMAIATTSFI YISCVVLFGA CFEGVVLRDK
YGDSVNKNLV VATLAWPSPW VIVIGSFFSC CGAGLQSLTG APRILQAIAR DGIMPFLQVF
GRSKSGGEPT WALLLTAAIC EIGILIASLD AVAPILSMFF LMCYLFVNLA CAVQTLLRTP
NWRPRFKFYH WSLSFLGMSL SLSLMFVSSW YYALVVILIA GCIYKYIEYR GAVKEWGDGL
RGLSLNAARY ALIKLEEAQP HTKNWRPQLL VLMKLDTDLT VKHPRLLSFT SQLKAGKGLT
IVCSVLEGTF MVRGADAKLA EQNIKAAMAK EKTKGFCHVV VSSNQRDGFS HLIQSAGLGG
MKHNAVLVAW PANWKQAESS LSWKNFIETV RETTAAHQAL LVAKNVDTFP SNQDRLAEGT
IDVWWIVHDG GLLMLLPFLL RQHKVWRKCK MRIFTVAQMD DNSIQMKKDL QMFLYHLRLD
AKVEVVEMNA GDISAFTYEK TLVMEQRSQM LKQMQLSKTE REREIQSISD ESRISIRRKP
CGEKQTHNSK LKVATVNLDE EAQLIHDKNT KSHATLVEKA TPTSDRVHMT WTKEKLIGER
NRLRDASMHV RDIFNMKPDQ TNVRRMHTAV KLNEVVVKKS KGAQLVLLNM PGPPKNKGGD
ENYMEFLEVL TEGLDHVLLV RGGGREVITI YS
//
