ID X4RAN6_9ACTO Unreviewed; 378 AA.
AC X4RAN6;
DT 11-JUN-2014, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=Beta sliding clamp {ECO:0000256|PIRNR:PIRNR000804};
GN Name=dnaN {ECO:0000313|EMBL:SUO88287.1};
GN ORFNames=B1R42_07990 {ECO:0000313|EMBL:OQD36082.1}, CT171_07545
GN {ECO:0000313|EMBL:PIN51530.1}, M5C92_00010
GN {ECO:0000313|EMBL:UVJ59763.1}, NCTC5224_01998
GN {ECO:0000313|EMBL:SUO88287.1};
OS Trueperella pyogenes.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Trueperella.
OX NCBI_TaxID=1661 {ECO:0000313|EMBL:SUO88287.1, ECO:0000313|Proteomes:UP000254485};
RN [1] {ECO:0000313|EMBL:OQD36082.1, ECO:0000313|Proteomes:UP000191477}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UFV1 {ECO:0000313|EMBL:OQD36082.1,
RC ECO:0000313|Proteomes:UP000191477};
RA Duarte V.S., Treu L., Campanaro S., Dias R.S., Da Silva C., Giacomini A.,
RA Corich V., De Paula S.O.;
RT "The complete genome sequence of Trueperella pyogenes UFV1 reveals an AI-2
RT transporter and processing system involved in quorum sensing signal-
RT response.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PIN51530.1, ECO:0000313|Proteomes:UP000228715}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bu5 {ECO:0000313|EMBL:PIN51530.1,
RC ECO:0000313|Proteomes:UP000228715};
RA Vaid R.K., Anand T., Riyesh T., Bera B.C., Shanmugasundaram K., Rana N.,
RA Bardwaj S., Batra P., Sharma T., Singha H., Singh R.K., Tripathi B.N.;
RT "First Draft genome sequence of Trueperella pyogenes strain Bu5 isolated
RT from water buffalo in India.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SUO88287.1, ECO:0000313|Proteomes:UP000254485}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC5224 {ECO:0000313|EMBL:SUO88287.1,
RC ECO:0000313|Proteomes:UP000254485};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:UVJ59763.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=22/KM0800 {ECO:0000313|EMBL:UVJ59763.1};
RA Marchionatti E., Perreten V.;
RT "Complete Genome Sequences of Trueperella pyogenes (tetW) from cattle in
RT Switzerland.";
RL Submitted (MAY-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Confers DNA tethering and processivity to DNA polymerases and
CC other proteins. Acts as a clamp, forming a ring around DNA (a reaction
CC catalyzed by the clamp-loading complex) which diffuses in an ATP-
CC independent manner freely and bidirectionally along dsDNA. Initially
CC characterized for its ability to contact the catalytic subunit of DNA
CC polymerase III (Pol III), a complex, multichain enzyme responsible for
CC most of the replicative synthesis in bacteria; Pol III exhibits 3'-5'
CC exonuclease proofreading activity. The beta chain is required for
CC initiation of replication as well as for processivity of DNA
CC replication. {ECO:0000256|PIRNR:PIRNR000804}.
CC -!- SUBUNIT: Forms a ring-shaped head-to-tail homodimer around DNA.
CC {ECO:0000256|PIRNR:PIRNR000804}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|PIRNR:PIRNR000804}.
CC -!- SIMILARITY: Belongs to the beta sliding clamp family.
CC {ECO:0000256|ARBA:ARBA00010752, ECO:0000256|PIRNR:PIRNR000804}.
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DR EMBL; MVGS01000066; OQD36082.1; -; Genomic_DNA.
DR EMBL; PESV01000003; PIN51530.1; -; Genomic_DNA.
DR EMBL; UHHW01000002; SUO88287.1; -; Genomic_DNA.
DR EMBL; CP097247; UVJ59763.1; -; Genomic_DNA.
DR RefSeq; WP_024963113.1; NZ_UHHW01000002.1.
DR AlphaFoldDB; X4RAN6; -.
DR STRING; 1661.CQ11_07600; -.
DR GeneID; 41087441; -.
DR OrthoDB; 468978at2; -.
DR Proteomes; UP000191477; Unassembled WGS sequence.
DR Proteomes; UP000228715; Unassembled WGS sequence.
DR Proteomes; UP000254485; Unassembled WGS sequence.
DR Proteomes; UP001058955; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00140; beta_clamp; 1.
DR Gene3D; 3.10.150.10; DNA Polymerase III, subunit A, domain 2; 3.
DR InterPro; IPR046938; DNA_clamp_sf.
DR InterPro; IPR001001; DNA_polIII_beta.
DR InterPro; IPR022635; DNA_polIII_beta_C.
DR InterPro; IPR022637; DNA_polIII_beta_cen.
DR InterPro; IPR022634; DNA_polIII_beta_N.
DR NCBIfam; TIGR00663; dnan; 1.
DR PANTHER; PTHR30478:SF0; BETA SLIDING CLAMP; 1.
DR PANTHER; PTHR30478; DNA POLYMERASE III SUBUNIT BETA; 1.
DR Pfam; PF00712; DNA_pol3_beta; 1.
DR Pfam; PF02767; DNA_pol3_beta_2; 1.
DR Pfam; PF02768; DNA_pol3_beta_3; 1.
DR PIRSF; PIRSF000804; DNA_pol_III_b; 1.
DR SMART; SM00480; POL3Bc; 1.
DR SUPFAM; SSF55979; DNA clamp; 3.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR000804};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|PIRNR:PIRNR000804};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|PIRNR:PIRNR000804};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|PIRNR:PIRNR000804};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000804}.
FT DOMAIN 1..119
FT /note="DNA polymerase III beta sliding clamp N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00712"
FT DOMAIN 130..247
FT /note="DNA polymerase III beta sliding clamp central"
FT /evidence="ECO:0000259|Pfam:PF02767"
FT DOMAIN 249..358
FT /note="DNA polymerase III beta sliding clamp C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02768"
SQ SEQUENCE 378 AA; 41071 MW; 68410237F74C284F CRC64;
MKFSVDHKEF TEAVTWASRT IPNRPANAVL AGIHIVAEED GTVTMGARDS DISSQISVDN
ADVVVPGEVL VNGKLLAEIS RSLPDQPIEI EKDGGKFEIS CGRSHFTLKT MATEDYADLP
AMPPVIGKVD GAEWEKAVSQ VTIAASNDDT LPMLVSVCIE IEGDQISLMA TDRYRLAIRD
LGWVPVKSDF STRILVRASR LLEVAKALGT VADVEISLQD QGMGALIGFK AANRQNTLQL
IDGEYPQVRS LFPSEVNGHV EMNRLEILEA IKRSRLVLEK NAAVRLSFSE GEVMLEAGQG
DNAQVSEILP ATLEGEELKM AFNPVFLQEG FSVINAPAVR LSFTHPTKPA VVTAQDEDGN
VDDAFRLLLM PIRTFGAN
//
