ID XERA_THEKO Reviewed; 282 AA.
AC Q5JHA3;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=Tyrosine recombinase XerA {ECO:0000255|HAMAP-Rule:MF_02055};
GN Name=xerA {ECO:0000255|HAMAP-Rule:MF_02055}; OrderedLocusNames=TK0777;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules.
CC {ECO:0000255|HAMAP-Rule:MF_02055}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02055}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02055}.
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DR EMBL; AP006878; BAD84966.1; -; Genomic_DNA.
DR RefSeq; WP_011249728.1; NC_006624.1.
DR AlphaFoldDB; Q5JHA3; -.
DR SMR; Q5JHA3; -.
DR STRING; 69014.TK0777; -.
DR EnsemblBacteria; BAD84966; BAD84966; TK0777.
DR GeneID; 78447292; -.
DR KEGG; tko:TK0777; -.
DR PATRIC; fig|69014.16.peg.757; -.
DR eggNOG; arCOG01241; Archaea.
DR HOGENOM; CLU_027562_9_5_2; -.
DR InParanoid; Q5JHA3; -.
DR OrthoDB; 142231at2157; -.
DR PhylomeDB; Q5JHA3; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:DNA transposition; IEA:UniProtKB-UniRule.
DR CDD; cd00798; INT_XerDC_C; 1.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR HAMAP; MF_02055; Recomb_XerA; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR033686; XerA.
DR NCBIfam; NF040815; recomb_XerA_Arch; 1.
DR PANTHER; PTHR30349:SF41; INTEGRASE_RECOMBINASE HI_1414-RELATED; 1.
DR PANTHER; PTHR30349; PHAGE INTEGRASE-RELATED; 1.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA integration; DNA recombination; DNA-binding;
KW Reference proteome.
FT CHAIN 1..282
FT /note="Tyrosine recombinase XerA"
FT /id="PRO_0000095358"
FT DOMAIN 2..79
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 95..271
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 132
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT ACT_SITE 157
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT ACT_SITE 223
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT ACT_SITE 226
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT ACT_SITE 249
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT ACT_SITE 258
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
SQ SEQUENCE 282 AA; 32751 MW; A4CCC0956E410E10 CRC64;
MSEPNEVIEE FETYLDLEGK SPHTIRMYTY YVRRYLEWGG DLNAHSALRF LAHLRKNGYS
NRSLNLVVQA LRAYFRFEGL DDEAERLKPP KVPRSLPKAL TREEVKRLLS VIPPTRKRDR
LIVLLLYGAG LRVSELCNLK KDDVDLDRGL IVVRGGKGAK DRVVPIPKYL ADEIRAYLES
RSDESEYLLV EDRRRRKDKL STRNVWYLLK RYGQKAGVEV TPHKLRHSFA THLLEEGVDI
RAIQELLGHS NLSTTQIYTK VTVEHLRKAQ EKAKLIEKLM GE
//
