ID XERC_PSEAE Reviewed; 303 AA.
AC Q51566; Q9HTS4;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 27-MAR-2024, entry version 139.
DE RecName: Full=Tyrosine recombinase XerC;
GN Name=xerC; Synonyms=sss; OrderedLocusNames=PA5280;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=7NSK2;
RX PubMed=7715441; DOI=10.1111/j.1365-2958.1994.tb01335.x;
RA Hoefte M., Dong Q., Kourambas S., Krishnapillai V., Sherratt D.J.,
RA Mergeay M.;
RT "The sss gene product, which affects pyoverdin production in Pseudomonas
RT aeruginosa 7NSK2, is a site-specific recombinase.";
RL Mol. Microbiol. 14:1011-1020(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION.
RX PubMed=10744667; DOI=10.1074/jbc.275.14.9930;
RA Blakely G.W., Davidson A.O., Sherratt D.J.;
RT "Sequential strand exchange by XerC and XerD during site-specific
RT recombination at dif.";
RL J. Biol. Chem. 275:9930-9936(2000).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. The XerC-
CC XerD complex is essential to convert dimers of the bacterial chromosome
CC into monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:10744667}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Defects affect induction of pyoverdin and related
CC outer membrane proteins. {ECO:0000269|PubMed:7715441}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC {ECO:0000305}.
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DR EMBL; X78478; CAA55226.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG08665.1; -; Genomic_DNA.
DR PIR; A82987; A82987.
DR PIR; S61402; S61402.
DR RefSeq; NP_253967.1; NC_002516.2.
DR RefSeq; WP_003114345.1; NZ_QZGE01000020.1.
DR AlphaFoldDB; Q51566; -.
DR SMR; Q51566; -.
DR STRING; 208964.PA5280; -.
DR PaxDb; 208964-PA5280; -.
DR DNASU; 878328; -.
DR GeneID; 878328; -.
DR KEGG; pae:PA5280; -.
DR PATRIC; fig|208964.12.peg.5534; -.
DR PseudoCAP; PA5280; -.
DR HOGENOM; CLU_027562_9_0_6; -.
DR InParanoid; Q51566; -.
DR OrthoDB; 9801717at2; -.
DR PhylomeDB; Q51566; -.
DR BioCyc; PAER208964:G1FZ6-5401-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0071294; P:cellular response to zinc ion; IMP:PseudoCAP.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:DNA transposition; IEA:UniProtKB-UniRule.
DR CDD; cd00798; INT_XerDC_C; 1.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR011931; Recomb_XerC.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR NCBIfam; TIGR02224; recomb_XerC; 1.
DR PANTHER; PTHR30349; PHAGE INTEGRASE-RELATED; 1.
DR PANTHER; PTHR30349:SF85; TYROSINE RECOMBINASE XERC; 1.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR SUPFAM; SSF47823; lambda integrase-like, N-terminal domain; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT CHAIN 1..303
FT /note="Tyrosine recombinase XerC"
FT /id="PRO_0000095314"
FT DOMAIN 1..85
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 106..285
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 146
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 170
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 237
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 240
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 263
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 272
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT CONFLICT 99
FT /note="S -> R (in Ref. 1; CAA55226)"
FT /evidence="ECO:0000305"
FT CONFLICT 286..288
FT /note="RAH -> API (in Ref. 1; CAA55226)"
FT /evidence="ECO:0000305"
FT CONFLICT 293..297
FT /note="RKGNA -> QGQR (in Ref. 1; CAA55226)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 303 AA; 33930 MW; 967F807FCC00832A CRC64;
MRADLDAFLE HLRSERQVSA HTLDGYRRDL LKILALAEKA GLSDWNALDT RSLRTFVARL
HQQGQSSRSL ARLLSATRGL YQYLLREGRC RHDPANGLSA PKSPRKLPRT LDADRALQLL
DGAVEDDFIA RRDQALLELF YSSGLRLSEL VGLDLEWLDL KEGLVRVRGK GNKVRELPVG
KAARQALEAW LPLRTQAAPE DGAVFIGRSG KRLTPRAIQL RVRQAGVREL GQHLHPHMLR
HSFASHLLES SGDLRAVQEL LGHADIATTQ IYTHLDFQHL ASVYDRAHPR AKRKGNADGG
NDP
//
