ID YB8B_YEAST Reviewed; 427 AA.
AC P38355; D6VQT2; E9P8W6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 24-JAN-2024, entry version 149.
DE RecName: Full=Uncharacterized transporter YBR287W;
GN OrderedLocusNames=YBR287W; ORFNames=YBR2034;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091861; DOI=10.1002/yea.320100007;
RA Holmstroem K., Brandt T., Kallesoe T.;
RT "The sequence of a 32,420 bp segment located on the right arm of chromosome
RT II from Saccharomyces cerevisiae.";
RL Yeast 10:S47-S62(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-199, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 6440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69) family.
CC {ECO:0000305}.
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DR EMBL; X76053; CAA53650.1; -; Genomic_DNA.
DR EMBL; Z36156; CAA85252.1; -; Genomic_DNA.
DR EMBL; AY692590; AAT92609.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07402.1; -; Genomic_DNA.
DR PIR; S44549; S44549.
DR RefSeq; NP_009846.1; NM_001178635.1.
DR AlphaFoldDB; P38355; -.
DR BioGRID; 32981; 76.
DR DIP; DIP-6660N; -.
DR IntAct; P38355; 6.
DR MINT; P38355; -.
DR STRING; 4932.YBR287W; -.
DR TCDB; 2.A.69.2.2; the auxin efflux carrier (aec) family.
DR CarbonylDB; P38355; -.
DR iPTMnet; P38355; -.
DR MaxQB; P38355; -.
DR PaxDb; 4932-YBR287W; -.
DR PeptideAtlas; P38355; -.
DR EnsemblFungi; YBR287W_mRNA; YBR287W; YBR287W.
DR GeneID; 852590; -.
DR KEGG; sce:YBR287W; -.
DR AGR; SGD:S000000491; -.
DR SGD; S000000491; YBR287W.
DR VEuPathDB; FungiDB:YBR287W; -.
DR eggNOG; KOG2722; Eukaryota.
DR HOGENOM; CLU_026460_2_0_1; -.
DR InParanoid; P38355; -.
DR OMA; IFVVQTF; -.
DR OrthoDB; 1430296at2759; -.
DR BioCyc; YEAST:G3O-29206-MONOMER; -.
DR BioGRID-ORCS; 852590; 0 hits in 10 CRISPR screens.
DR PRO; PR:P38355; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38355; Protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR004776; Mem_transp_PIN-like.
DR PANTHER; PTHR31794; AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG); 1.
DR PANTHER; PTHR31794:SF2; AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG); 1.
DR Pfam; PF03547; Mem_trans; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..427
FT /note="Uncharacterized transporter YBR287W"
FT /id="PRO_0000123805"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 199
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 425
FT /note="N -> K (in Ref. 4; AAT92609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 427 AA; 47506 MW; A2F947D0EA90E41C CRC64;
MVETFSFAHL AYLVFESVLQ VVIIALAGFW SASSGLLPKQ SQKIISLLNV DLFTPCLIFS
KLAKSLSMAK IFEIAIIPIF FGLTTGISFI SGKIMSRILD LDKDETNFVV ANSVFGNSNS
LPVSLTLSLA YTLPNLTWDQ IPNDNRDNVA SRGILYLLIF QQIGQMLRWS WGYNKLMKWS
GENTQHMPPS QVQSLLERTP NIDNEELVNE EQEEQELLEE ENNRMNSSFL SSSSIGDKIW
QKSCTVFERI RANLNPPLYS MIFAVVVAAI GPLQRELFME DGFINNTFAE AVTQLGSVSI
PLILVVLGSN LYPSAEVFPK TVHHSKLLIG SIIGRMILPS CFLLPIIAIA VKYINVSILD
DPIFLVVGFL LTVSPPAIQL TQITQLNEFF EAEMADILFW GYAVLSLPVS IIVVSGAIYV
LQWANPT
//
