ID YDAF_BACSU Reviewed; 183 AA.
AC P96579; Q797M8;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 27-MAR-2024, entry version 122.
DE RecName: Full=Putative ribosomal N-acetyltransferase YdaF;
DE EC=2.3.1.-;
GN Name=ydaF; OrderedLocusNames=BSU04210;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), FUNCTION, AND SUBUNIT.
RX PubMed=15468321; DOI=10.1002/prot.10601;
RA Brunzelle J.S., Wu R., Korolev S.V., Collart F.R., Joachimiak A.,
RA Anderson W.F.;
RT "Crystal structure of Bacillus subtilis YdaF protein: a putative ribosomal
RT N-acetyltransferase.";
RL Proteins 57:850-853(2004).
CC -!- FUNCTION: Putative N-acetyltransferase. May act on ribosomal proteins
CC (Potential). {ECO:0000305}.
CC -!- SUBUNIT: Homohexamer, and homodimer. {ECO:0000269|PubMed:15468321}.
CC -!- SIMILARITY: Belongs to the acetyltransferase family. {ECO:0000305}.
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DR EMBL; AB001488; BAA19259.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12228.1; -; Genomic_DNA.
DR PIR; F69768; F69768.
DR RefSeq; NP_388302.1; NC_000964.3.
DR RefSeq; WP_003246691.1; NZ_JNCM01000031.1.
DR PDB; 1NSL; X-ray; 2.70 A; A/B/C/D/E/F=1-183.
DR PDBsum; 1NSL; -.
DR AlphaFoldDB; P96579; -.
DR SMR; P96579; -.
DR STRING; 224308.BSU04210; -.
DR PaxDb; 224308-BSU04210; -.
DR EnsemblBacteria; CAB12228; CAB12228; BSU_04210.
DR GeneID; 938250; -.
DR KEGG; bsu:BSU04210; -.
DR PATRIC; fig|224308.179.peg.447; -.
DR eggNOG; COG1670; Bacteria.
DR InParanoid; P96579; -.
DR OrthoDB; 9784707at2; -.
DR PhylomeDB; P96579; -.
DR BioCyc; BSUB:BSU04210-MONOMER; -.
DR EvolutionaryTrace; P96579; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008999; F:peptide-alanine-alpha-N-acetyltransferase activity; IBA:GO_Central.
DR GO; GO:1990189; F:peptide-serine-alpha-N-acetyltransferase activity; IBA:GO_Central.
DR CDD; cd04301; NAT_SF; 1.
DR Gene3D; 3.40.630.30; -; 1.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR PANTHER; PTHR43441; RIBOSOMAL-PROTEIN-SERINE ACETYLTRANSFERASE; 1.
DR PANTHER; PTHR43441:SF10; RIBOSOMAL-PROTEIN-SERINE ACETYLTRANSFERASE; 1.
DR Pfam; PF13302; Acetyltransf_3; 1.
DR SUPFAM; SSF55729; Acyl-CoA N-acyltransferases (Nat); 1.
DR PROSITE; PS51186; GNAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..183
FT /note="Putative ribosomal N-acetyltransferase YdaF"
FT /id="PRO_0000360208"
FT DOMAIN 10..176
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 7..13
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 20..28
FT /evidence="ECO:0007829|PDB:1NSL"
FT TURN 29..37
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 47..52
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 68..74
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 77..88
FT /evidence="ECO:0007829|PDB:1NSL"
FT TURN 89..92
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 109..123
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 128..135
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 139..148
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 151..162
FT /evidence="ECO:0007829|PDB:1NSL"
FT STRAND 165..175
FT /evidence="ECO:0007829|PDB:1NSL"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:1NSL"
SQ SEQUENCE 183 AA; 21025 MW; C1BC09DF75A86373 CRC64;
MFTCKVNEHI TIRLLEPKDA ERLAELIIQN QQRLGKWLFF AENPSSADTY RETIIPDWRR
QYADLNGIEA GLLYDGSLCG MISLHNLDQV NRKAEIGYWI AKEFEGKGII TAACRKLITY
AFEELELNRV AICAAVGNEK SRAVPERIGF LEEGKARDGL YVNGMHHDLV YYSLLKREWE
GEK
//
