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Database: UniProt
Entry: YDAP_BACSU
LinkDB: YDAP_BACSU
Original site: YDAP_BACSU 
ID   YDAP_BACSU              Reviewed;         574 AA.
AC   P96591; Q797M1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   23-MAY-2018, entry version 106.
DE   RecName: Full=Putative thiamine pyrophosphate-containing protein YdaP;
GN   Name=ydaP; OrderedLocusNames=BSU04340;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the
RT   Bacillus subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   REGULATION.
RC   STRAIN=168;
RX   PubMed=10220166; DOI=10.1099/13500872-145-4-869;
RA   Petersohn A., Antelmann H., Gerth U., Hecker M.;
RT   "Identification and transcriptional analysis of new members of the
RT   sigmaB regulon in Bacillus subtilis.";
RL   Microbiology 145:869-880(1999).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000305};
CC   -!- COFACTOR:
CC       Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC         Evidence={ECO:0000305};
CC       Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000305};
CC   -!- INDUCTION: Transcriptionally regulated by SigB.
CC   -!- SIMILARITY: Belongs to the TPP enzyme family. {ECO:0000305}.
DR   EMBL; AB001488; BAA19271.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12241.1; -; Genomic_DNA.
DR   PIR; G69769; G69769.
DR   RefSeq; NP_388315.1; NC_000964.3.
DR   RefSeq; WP_003246569.1; NZ_JNCM01000031.1.
DR   ProteinModelPortal; P96591; -.
DR   SMR; P96591; -.
DR   IntAct; P96591; 1.
DR   STRING; 224308.Bsubs1_010100002458; -.
DR   PaxDb; P96591; -.
DR   PRIDE; P96591; -.
DR   EnsemblBacteria; CAB12241; CAB12241; BSU04340.
DR   GeneID; 938239; -.
DR   KEGG; bsu:BSU04340; -.
DR   PATRIC; fig|224308.179.peg.460; -.
DR   eggNOG; COG0028; LUCA.
DR   HOGENOM; HOG000258444; -.
DR   InParanoid; P96591; -.
DR   KO; K00158; -.
DR   OMA; RGKEWIQ; -.
DR   PhylomeDB; P96591; -.
DR   BioCyc; BSUB:BSU04340-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR011766; TPP_enzyme-bd_C.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   SUPFAM; SSF52518; SSF52518; 2.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Complete proteome; FAD; Flavoprotein; Magnesium;
KW   Metal-binding; Reference proteome; Thiamine pyrophosphate.
FT   CHAIN         1    574       Putative thiamine pyrophosphate-
FT                                containing protein YdaP.
FT                                /FTId=PRO_0000380114.
FT   NP_BIND     256    277       FAD. {ECO:0000250}.
FT   NP_BIND     294    313       FAD. {ECO:0000250}.
FT   REGION      384    464       Thiamine pyrophosphate binding.
FT                                {ECO:0000250}.
FT   COILED       28     55       {ECO:0000255}.
FT   METAL       435    435       Magnesium. {ECO:0000250}.
FT   METAL       462    462       Magnesium. {ECO:0000250}.
FT   BINDING      52     52       Thiamine pyrophosphate. {ECO:0000250}.
SQ   SEQUENCE   574 AA;  63138 MW;  F887A1271B7E9710 CRC64;
     MAHKTAGQAM TELLEQWGVD HVYGIPGDSI NEFIEELRHE RNQLKFIQTR HEEVAALAAA
     AEAKLTGKIG VCLSIAGPGA VHLLNGLYDA KADGAPVLAI AGQVSSGEVG RDYFQEIKLE
     QMFEDVAVFN REVHSAESLP DLLNQAIRTA YSKKGVAVLS VSDDLFAEKI KREPVYTSPV
     YIEGNLEPKK EQLVTCAQYI NNAKKPIILA GQGMKKAKRE LLEFADKAAA PIVVTLPAKG
     VVPDKHPHFL GNLGQIGTKP AYEAMEECDL LIMLGTSFPY RDYLPDDTPA IQLDSDPAKI
     GKRYPVTAGL VCDSALGLRE LTEYIERKED RRFLNACTEH MQHWWNEIEK DETEATTPLK
     PQQVVARLQE AAADDAVLSV DVGTVTVWMA RHFKMNANQD FIVSSWLATM GCGLPGAIAA
     SLSEPERQAI AVCGDGGFSM VMQDLPTAVK YKLPITVVIL NNENLGMIEY EQQVKGNIDY
     VTKLQNVDYA AFAESCGAKG IKVTKAEELA PAFHEALHSD QPVVVDVMIG NEPPLPGKIT
     YGQAKGFSKY MLKNFFENQK FEMPSLKKSL KRLF
//
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