ID YUKB_BACSU Reviewed; 1495 AA.
AC C0SPA7; P71069; Q795L5; Q798N1;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=ESX secretion system protein YukB {ECO:0000305};
GN Name=yukB; Synonyms=yukA, yukBA; OrderedLocusNames=BSU31875;
GN ORFNames=BSU31880;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-896.
RC STRAIN=168;
RX PubMed=9168598; DOI=10.1099/00221287-143-5-1489;
RA Oudega B., Vandenbol M., Koningstein G.;
RT "A 12kb nucleotide sequence containing the alanine dehydrogenase gene at
RT 279 degree on the Bacillus subtilis chromosome.";
RL Microbiology 143:1489-1491(1997).
RN [4]
RP IDENTIFICATION OF FRAMESHIFT, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=15576783; DOI=10.1128/jb.186.24.8337-8346.2004;
RA Sao-Jose C., Baptista C., Santos M.A.;
RT "Bacillus subtilis operon encoding a membrane receptor for bacteriophage
RT SPP1.";
RL J. Bacteriol. 186:8337-8346(2004).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168, and ATCC 6051;
RX PubMed=23861817; DOI=10.1371/journal.pone.0067840;
RA Baptista C., Barreto H.C., Sao-Jose C.;
RT "High levels of DegU-P activate an Esat-6-like secretion system in Bacillus
RT subtilis.";
RL PLoS ONE 8:E67840-E67840(2013).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / PY79;
RX PubMed=24798022; DOI=10.1371/journal.pone.0096267;
RA Huppert L.A., Ramsdell T.L., Chase M.R., Sarracino D.A., Fortune S.M.,
RA Burton B.M.;
RT "The ESX system in Bacillus subtilis mediates protein secretion.";
RL PLoS ONE 9:E96267-E96267(2014).
CC -!- FUNCTION: Required for YukE secretion. Probable component or regulator
CC of the ESX/ESAT-6-like secretion system (BsEss).
CC {ECO:0000269|PubMed:23861817, ECO:0000269|PubMed:24798022}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are blocked in YukE
CC secretion (PubMed:23861817, PubMed:24798022). They display an increased
CC bacteriophage SPP1 resistance phenotype (PubMed:15576783).
CC {ECO:0000269|PubMed:15576783, ECO:0000269|PubMed:23861817,
CC ECO:0000269|PubMed:24798022}.
CC -!- SIMILARITY: Belongs to the EssC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB04781.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAB04782.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB04782.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL009126; CAB15176.2; -; Genomic_DNA.
DR EMBL; Z82015; CAB04781.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Z82015; CAB04782.1; ALT_SEQ; Genomic_DNA.
DR PIR; D70013; D70013.
DR RefSeq; NP_391066.2; NC_000964.3.
DR RefSeq; WP_003243866.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; C0SPA7; -.
DR SMR; C0SPA7; -.
DR STRING; 224308.BSU31875; -.
DR PaxDb; 224308-BSU31875; -.
DR EnsemblBacteria; CAB15176; CAB15176; BSU_31875.
DR GeneID; 936565; -.
DR KEGG; bsu:BSU31875; -.
DR PATRIC; fig|224308.179.peg.3453; -.
DR eggNOG; COG0433; Bacteria.
DR eggNOG; COG1674; Bacteria.
DR InParanoid; C0SPA7; -.
DR OrthoDB; 9807790at2; -.
DR PhylomeDB; C0SPA7; -.
DR BioCyc; BSUB:BSU31875-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR CDD; cd00060; FHA; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR023839; Firmicutes_EssC_C.
DR InterPro; IPR022206; Firmicutes_EssC_N.
DR InterPro; IPR002543; FtsK_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR03928; T7_EssCb_Firm; 1.
DR PANTHER; PTHR22683; SPORULATION PROTEIN RELATED; 1.
DR PANTHER; PTHR22683:SF1; TYPE VII SECRETION SYSTEM PROTEIN ESSC; 1.
DR Pfam; PF01580; FtsK_SpoIIIE; 3.
DR Pfam; PF12538; FtsK_SpoIIIE_N; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR PROSITE; PS50901; FTSK; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..1495
FT /note="ESX secretion system protein YukB"
FT /id="PRO_0000383633"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 661..858
FT /note="FtsK 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT DOMAIN 993..1177
FT /note="FtsK 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 682..689
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 1010..1017
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT CONFLICT 119
FT /note="I -> V (in Ref. 3; CAB04782)"
FT /evidence="ECO:0000305"
FT CONFLICT 469..470
FT /note="WL -> CV (in Ref. 3; CAB04781)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1495 AA; 171851 MW; 4E302D3699AD9F6D CRC64;
MSLLWVFYQN NVQKLNLSNL PSSHPVTIGP DVKDSVTIST IPFNSGVISL KRKESGGQYE
VFLGNDCLGT IETDISFTLQ TDQQDIRLIL TGSEPEKSVY FTGNRDEIVC SSEKTNADIY
LNPQDFAFAE QSTFSLLRAG GSWSVRPESG TIFLNGEKIN ANTPLKPGDE IFWNFTQMRV
TEQDLLEIVH YAQFETALTE TVKPSTEMQK KYPQYRRTPR MVYDLPDDRV SFSFPSQESD
QTNRGLWLVI LPPLVMLIVM GVVAIIQPRG IFILVSLAMF MMTLITSTVQ YFRDKNQRKK
REEKRERVYK LYLDNKRKEL QALAEKQKQV LEFHFPSFEQ MKYLTSEISD RIWEKSLESK
DYLQLRLGTG TVPSSYEINM SGGDLANRDI DDLMEKSQHM QRVYKDIRNA PVTVDLAEGP
MGLVGKSQIV KNEIHQLIGQ LSFFNSYHDL RFVFIFHEEE YKDWEWMKWL PQFQMPHIYA
KGFIYNEQTR DQLLSSLYEL IRERDLEDDK EKLQFKPHFV FVITNQQLIS EHVILEYLEG
QHEHLGISTI VAAETKESLS ENITTLVRYI NEHEGDILIQ KKKAVRIPFR LDHHQREDNE
RFSRTLRTLN HQVGITNSIP ETVSFLELFH AKEVKEIGIQ QRWLTSESSK SLSVPIGYKG
KDDIVYLNLH EKAHGPHGLL AGTTGSGKSE FLQTYILSLA VHFHPHEAAF LLIDYKGGGM
AQPFRNIPHL LGTITNIEGS KNFSMRALAS IKSELKKRQR LFDQYQVNHI NDYTKLYKQG
KAEVAMPHLF LISDEFAELK SEEPDFIREL VSAARIGRSL GVHLILATQK PGGIIDDQIW
SNSRFKVALK VQDATDSKEI LKNSDAANIT VTGRGYLQVG NNEVYELFQS AWSGAPYLEE
VYGTEDEIAI VTDTGLIPLS EVDTEDNAKK DVQTEIEAVV DEIERIQDEM GIEKLPSPWL
PPLAERIPRT LFPSNEKDHF HFAYVDEPDL QRQAPIAYKM MEDGNIGIFG SSGYGKSIAA
ATFLMSFADV YTPEELHVYI FDFGNGTLLP LAKLPHTADY FLMDQSRKIE KFMIRIKEEI
DRRKRLFREK EISHIKMYNA LSEEELPFIF ITIDNFDIVK DEMHELESEF VQLSRDGQSL
GIYFMLTATR VNAVRQSLLN NLKTKIVHYL MDQSEGYSIY GRPKFNLEPI PGRVIIQKEE
LYFAQMFLPV DADDDIGMFN ELKSDVQKLQ GRFASMEQPA PIPMLPESLS TRELSIRFKL
ERKPLSVPIG LHEETVSPVY FDLGKHKHCL ILGQTQRGKT NVLKVMLEHL IDDETEMIGL
FDSIDRGLSH YAKESDVSYL ETKEDIEQWI ETAEDIFKTR EAMYVEAVRQ GDAQNLRFSQ
VVLMIDGITR FQQTIDTRIQ DRLANFMKSY AHLGFSFIPG GNHSEFSKGY DSLTTEMKQI
RHAILLMKKS EQNVIPLPYQ RQEPEIQPGF GYVVENGKEQ KVQIPLCSAE RESAR
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