ID ZFY2_MOUSE Reviewed; 777 AA.
AC P20662; Q3V0D8;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 27-MAR-2024, entry version 169.
DE RecName: Full=Zinc finger Y-chromosomal protein 2;
GN Name=Zfy2; Synonyms=Zfy-2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2493989; DOI=10.1016/0092-8674(89)90680-6;
RA Mardon G., Page D.C.;
RT "The sex-determining region of the mouse Y chromosome encodes a protein
RT with a highly acidic domain and 13 zinc fingers.";
RL Cell 56:765-770(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: Probable transcriptional activator. Binds to the consensus
CC sequence 5'-AGGCCY-3' (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DOMAIN: The binding of ZFY to DNA is mediated by the interaction of the
CC GGCC core base pairs with zinc fingers 12 and 13. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. ZFX/ZFY subfamily. {ECO:0000305}.
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DR EMBL; M24401; AAA56845.1; -; mRNA.
DR EMBL; AK133222; BAE21566.1; -; mRNA.
DR CCDS; CCDS41217.1; -.
DR PIR; A31491; A31491.
DR RefSeq; NP_033597.2; NM_009571.2.
DR AlphaFoldDB; P20662; -.
DR SMR; P20662; -.
DR STRING; 10090.ENSMUSP00000139591; -.
DR iPTMnet; P20662; -.
DR PhosphoSitePlus; P20662; -.
DR MaxQB; P20662; -.
DR PaxDb; 10090-ENSMUSP00000111557; -.
DR ProteomicsDB; 275063; -.
DR DNASU; 22768; -.
DR Ensembl; ENSMUST00000115891.2; ENSMUSP00000111557.2; ENSMUSG00000000103.13.
DR Ensembl; ENSMUST00000187148.7; ENSMUSP00000139591.2; ENSMUSG00000000103.13.
DR GeneID; 22768; -.
DR KEGG; mmu:22768; -.
DR UCSC; uc009uzp.1; mouse.
DR AGR; MGI:99213; -.
DR CTD; 22768; -.
DR MGI; MGI:99213; Zfy2.
DR VEuPathDB; HostDB:ENSMUSG00000000103; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000164731; -.
DR HOGENOM; CLU_021097_0_0_1; -.
DR InParanoid; P20662; -.
DR OMA; HQESKSH; -.
DR OrthoDB; 4793691at2759; -.
DR PhylomeDB; P20662; -.
DR TreeFam; TF335557; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 22768; 2 hits in 76 CRISPR screens.
DR ChiTaRS; Zfy2; mouse.
DR PRO; PR:P20662; -.
DR Proteomes; UP000000589; Chromosome Y.
DR RNAct; P20662; Protein.
DR Bgee; ENSMUSG00000000103; Expressed in seminiferous tubule of testis and 11 other cell types or tissues.
DR Genevisible; P20662; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009048; P:dosage compensation by inactivation of X chromosome; IDA:MGI.
DR GO; GO:0051598; P:meiotic recombination checkpoint signaling; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; IDA:MGI.
DR GO; GO:0007283; P:spermatogenesis; IDA:MGI.
DR Gene3D; 3.30.160.60; Classic Zinc Finger; 8.
DR InterPro; IPR006794; Transcrp_activ_Zfx/Zfy-dom.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR23226; ZINC FINGER AND SCAN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR23226:SF375; ZINC FINGER PROTEIN X-LINKED; 1.
DR Pfam; PF00096; zf-C2H2; 6.
DR Pfam; PF04704; Zfx_Zfy_act; 1.
DR SMART; SM00355; ZnF_C2H2; 12.
DR SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 6.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 9.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..777
FT /note="Zinc finger Y-chromosomal protein 2"
FT /id="PRO_0000047264"
FT ZN_FING 403..425
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 434..456
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 491..514
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 520..542
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 548..571
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 577..599
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 605..628
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 634..656
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 662..685
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 691..713
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 719..742
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 748..770
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 207..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 372..382
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT CONFLICT 158
FT /note="I -> T (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="R -> K (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
FT CONFLICT 480
FT /note="T -> TLCTHKT (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
FT CONFLICT 580
FT /note="G -> D (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
FT CONFLICT 670
FT /note="K -> N (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
FT CONFLICT 777
FT /note="A -> L (in Ref. 1; AAA56845)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 777 AA; 88127 MW; 0523462C0380E4DD CRC64;
MDEDEIELTP EEEKSLFDGI GADAVHMDSD QISVEVQETV FLSNSDVTVH NFVPDDPDSV
IIQDVIENVL IEDVHCSHIL EETDISDNVI IPEQVLDLDT AEEVSLAQFL IPDILTSSIT
STSLTMPEHV LMSEAIHVSN VGHFEQVIHD SLVEREIITD PLTADISDIL VADWASEAVL
DSSGMPLEQQ DDARINCEDY LMMSLDEPSK TDHEGSSEVT MNAESETDSS KLDEASPEVI
KVCILKADSE VDDVGETIQA VESETDNGNE AEVTDQRTSI HVPRVNIYML ASDSQKEEED
TKVIVGDEDA GGTAADTPEH EQQMDVSEIK AAFLPIAWTA AYDNNSDEIE VQNATASAML
HHDESGGLDR VPKQKSKKKK RPESKQYQSA IFVAPDGQTL RVYPCMFCGK KFKTKRFLKR
HIKNHPEYLA NKKYHCTECD YSTNKKISLH NHMESHKLTI KTEKTTECDD CRKNLSHAGT
MHTEKGVNKT CKCKFCDYET AEQTLLNHHL LVVHRKKFPH ICGECGKGFR HPSALKKHIR
VHTGEKPYEC QYCEYKSADS SNLKTHIKSK HSKEIPLKCG ICLLTFSDTK EAQQHAVLHQ
ESRTHQCSHC NHKSSNSSDL KRHIISVHTK AYPHKCDMCS KGFHRPSELK KHVATHKSKK
MHQCRHCDFK SPDPFLLSHH ILSAHTKNVP FKCKRCKKEF QQQCELQTHM KTHSSRKVYQ
CEYCEYSTKD ASGFKRHVIS IHTKDYPHRC DFCKKGFRRP SEKNQHIMRH HKEVGLA
//
