ID ZN474_MOUSE Reviewed; 347 AA.
AC Q6V5K9; Q9D472; Q9D985;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-NOV-2008, sequence version 2.
DT 27-MAR-2024, entry version 129.
DE RecName: Full=Zinc finger protein 474;
DE AltName: Full=Testis-specific zinc finger protein;
DE Short=TSZFP;
GN Name=Znf474; Synonyms=Zfp474;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RA Zhou C., Li L.-Y., Mo Y.-Q., Lu G.-X.;
RT "Identification of a novel mouse protein with two C2H2 zinc finger
RT domains.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU01371};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY350709; AAQ88275.1; -; mRNA.
DR EMBL; AK007266; BAB24926.2; -; mRNA.
DR EMBL; AK016749; BAB30408.1; -; mRNA.
DR EMBL; AC161119; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466528; EDL09922.1; -; Genomic_DNA.
DR EMBL; BC055099; AAH55099.1; -; mRNA.
DR CCDS; CCDS29248.1; -.
DR RefSeq; NP_080025.3; NM_025749.3.
DR RefSeq; XP_006526221.1; XM_006526158.2.
DR RefSeq; XP_006526222.1; XM_006526159.2.
DR RefSeq; XP_006526223.1; XM_006526160.2.
DR RefSeq; XP_006526224.1; XM_006526161.2.
DR RefSeq; XP_006526225.1; XM_006526162.2.
DR RefSeq; XP_006526228.1; XM_006526165.2.
DR RefSeq; XP_011245285.1; XM_011246983.2.
DR RefSeq; XP_011245286.1; XM_011246984.2.
DR AlphaFoldDB; Q6V5K9; -.
DR STRING; 10090.ENSMUSP00000147873; -.
DR PhosphoSitePlus; Q6V5K9; -.
DR PaxDb; 10090-ENSMUSP00000072453; -.
DR ProteomicsDB; 275290; -.
DR Antibodypedia; 25613; 54 antibodies from 12 providers.
DR DNASU; 66758; -.
DR Ensembl; ENSMUST00000072666.4; ENSMUSP00000072453.4; ENSMUSG00000046886.7.
DR Ensembl; ENSMUST00000209270.2; ENSMUSP00000147873.2; ENSMUSG00000046886.7.
DR GeneID; 66758; -.
DR KEGG; mmu:66758; -.
DR UCSC; uc008exh.2; mouse.
DR AGR; MGI:1914008; -.
DR CTD; 66758; -.
DR MGI; MGI:1914008; Zfp474.
DR VEuPathDB; HostDB:ENSMUSG00000046886; -.
DR eggNOG; ENOG502QWEF; Eukaryota.
DR GeneTree; ENSGT00940000164652; -.
DR HOGENOM; CLU_882643_0_0_1; -.
DR InParanoid; Q6V5K9; -.
DR OMA; RSCKTQP; -.
DR OrthoDB; 118932at2759; -.
DR PhylomeDB; Q6V5K9; -.
DR TreeFam; TF328720; -.
DR BioGRID-ORCS; 66758; 2 hits in 77 CRISPR screens.
DR ChiTaRS; Zfp474; mouse.
DR PRO; PR:Q6V5K9; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q6V5K9; Protein.
DR Bgee; ENSMUSG00000046886; Expressed in seminiferous tubule of testis and 26 other cell types or tissues.
DR Genevisible; Q6V5K9; MM.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.160.60; Classic Zinc Finger; 4.
DR InterPro; IPR026319; ZC2HC1A/B-like.
DR PANTHER; PTHR13555; C2H2 ZINC FINGER CGI-62-RELATED; 1.
DR PANTHER; PTHR13555:SF36; ZINC FINGER PROTEIN 474; 1.
DR Pfam; PF13913; zf-C2HC_2; 4.
DR PROSITE; PS52027; ZF_C2HC_C3H; 4.
PE 2: Evidence at transcript level;
KW Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..347
FT /note="Zinc finger protein 474"
FT /id="PRO_0000353099"
FT ZN_FING 90..119
FT /note="C2HC/C3H-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT ZN_FING 161..190
FT /note="C2HC/C3H-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT ZN_FING 217..246
FT /note="C2HC/C3H-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT ZN_FING 280..309
FT /note="C2HC/C3H-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT REGION 124..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 255..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 301..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 94
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 97
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 109
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 113
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 165
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 180
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 184
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 221
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 236
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 240
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 284
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 287
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 299
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01371"
FT CONFLICT 145
FT /note="H -> Y (in Ref. 1; AAQ88275)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 347 AA; 38134 MW; 1A08ABFE7AC36A82 CRC64;
MESGNERISS QSQGTIHLSK EPTFLIQQAT LPSDLHSTLL QETQCGGLTK NIKANTQKRR
PGTVILSKRS SRIMSETQPR PPVIPSRRPG FRICYICGRE FGSQSLAIHE PQCLEKWRTE
NSKLPKHLRR PEPSKPQPIG GTDSHSLQAA NEEAFQSAQA QLLPCENCGR TFLPDRLLVH
QRSCKPKGEN PGPPSMGSSN VPTGLKKASS GIPARPRTLI CYICGREFGT LSLPIHEPKC
LEKWKIENDQ LPRELRRPQP QKPQPLPAGQ SSQEGASQAA LVPCPNCGRT FAVDRLPVHQ
RSCKSQPSGP KTSNSNIERK GGPNPPTNSK QQRNMEAPNG DKVTGVI
//
