ID ZNUA_BRUA2 Reviewed; 334 AA.
AC Q2YJH5; Q58J93;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 27-MAR-2024, entry version 90.
DE RecName: Full=High-affinity zinc uptake system protein ZnuA;
DE Flags: Precursor;
GN Name=znuA; OrderedLocusNames=BAB2_1079;
OS Brucella abortus (strain 2308).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16790759; DOI=10.1128/iai.01957-05;
RA Yang X., Becker T., Walters N., Pascual D.W.;
RT "Deletion of znuA virulence factor attenuates Brucella abortus and confers
RT protection against wild-type challenge.";
RL Infect. Immun. 74:3874-3879(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
CC -!- FUNCTION: Part of the ATP-binding cassette (ABC) transport system
CC ZnuABC involved in zinc import (PubMed:16790759). Binds zinc with high
CC affinity and specificity and delivers it to the membrane permease for
CC translocation into the cytoplasm (PubMed:16790759). Required for
CC survival and normal growth under low Zn (2+) concentrations
CC (PubMed:16790759). Also required for virulence and intracellular growth
CC in macrophages (PubMed:16790759). {ECO:0000269|PubMed:16790759}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:A1B9L0}.
CC -!- DISRUPTION PHENOTYPE: Mutant strain (znuA delection) is attenuated in
CC infected mice, showing decreased splenic CFU. Also confers a protective
CC efficacy similar to that of RB51 and S19 vaccine strains.
CC {ECO:0000269|PubMed:16790759}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 9 family.
CC {ECO:0000305}.
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DR EMBL; AY941821; AAX46323.1; -; Genomic_DNA.
DR EMBL; AM040265; CAJ13245.1; -; Genomic_DNA.
DR RefSeq; WP_002966661.1; NZ_KN046823.1.
DR AlphaFoldDB; Q2YJH5; -.
DR SMR; Q2YJH5; -.
DR STRING; 359391.BAB2_1079; -.
DR GeneID; 3827700; -.
DR KEGG; bmf:BAB2_1079; -.
DR PATRIC; fig|359391.11.peg.1863; -.
DR HOGENOM; CLU_016838_1_2_5; -.
DR PhylomeDB; Q2YJH5; -.
DR PRO; PR:Q2YJH5; -.
DR Proteomes; UP000002719; Chromosome II.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR CDD; cd01019; ZnuA; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 2.
DR InterPro; IPR035520; ZnuA.
DR InterPro; IPR006127; ZnuA-like.
DR PANTHER; PTHR42953:SF3; HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA; 1.
DR PANTHER; PTHR42953; HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA-RELATED; 1.
DR Pfam; PF01297; ZnuA; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Ion transport; Metal-binding; Periplasm;
KW Reference proteome; Signal; Transport; Virulence; Zinc; Zinc transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..334
FT /note="High-affinity zinc uptake system protein ZnuA"
FT /id="PRO_0000248947"
FT REGION 120..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 60
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P39172"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P39172"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P39172"
FT BINDING 232
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P39172"
FT DISULFID 277..331
FT /evidence="ECO:0000250|UniProtKB:P39172"
FT CONFLICT 266
FT /note="I -> T (in Ref. 1; AAX46323)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 36046 MW; F3C873631569099F CRC64;
MKNLHSLFLA SAFLAGFCGS SLAGEREGVV VSIKPLHSIV SAVMQGVGKP KLIVQGAGSE
HVYSLKPSDA EAIEHAKVIF WAGPSMETFL DKPIDTLGEG AKVVALGDAK GLTKLKFREG
GPFEAHDHGH GGSHEEEHDA HGSGDHDHAA EVAEEGHEHH HHGEYDLHFW LDPQNGKILA
ADIAKTLGES DPEHAAQYEK NAKAYGEKLD ALTREVAAEL KPVKDKPFIV FHDAYQYFEN
RFGMKAAGSI TVSPEKAPGA ARIQQIHDKI KSLGATCVFS EPQFEPKLVK TVVDGTKART
GVLDPLGAEL KDGPDLYPQL IRNLANSLKD CLPK
//
