KEGG   ENZYME: 1.1.3.29
Entry
EC 1.1.3.29                 Enzyme                                 
Name
N-acylhexosamine oxidase;
N-acyl-D-hexosamine oxidase;
N-acyl-beta-D-hexosamine:oxygen 1-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
N-acyl-D-hexosamine:oxygen 1-oxidoreductase
Reaction(IUBMB)
(1) N-acetyl-D-glucosamine + O2 + H2O = N-acetyl-D-glucosaminate + H2O2 (overall reaction) [RN:R01203];
(1a) N-acetyl-D-glucosamine + O2 = N-acetyl-D-glucosamino-1,5-lactone + H2O2 [RN:R12973];
(1b) N-acetyl-D-glucosamino-1,5-lactone + H2O = N-acetyl-D-glucosaminate (spontaneous) [RN:R12974];
(2) N-acetyl-D-galactosamine + O2 + H2O = N-acetyl-D-galacotsaminate + H2O2 (overall reaction) [RN:R12975];
(2a) N-acetyl-D-galactosamine + O2 = N-acetyl-D-galactosamino-1,5-lactone + H2O2 [RN:R12976];
(2b) N-acetyl-D-galactosamino-1,5-lactone + H2O = N-acetyl-D-galactosaminate (spontaneous) [RN:R12977]
Reaction(KEGG)
Substrate
N-acetyl-D-glucosamine [CPD:C00140];
O2 [CPD:C00007];
H2O [CPD:C00001];
N-acetyl-D-glucosamino-1,5-lactone [CPD:C22488];
N-acetyl-D-galactosamine [CPD:C01132];
N-acetyl-D-galactosamino-1,5-lactone [CPD:C22489]
Product
N-acetyl-D-glucosaminate [CPD:C01133];
H2O2 [CPD:C00027];
N-acetyl-D-glucosamino-1,5-lactone [CPD:C22488];
N-acetyl-D-galacotsaminate;
N-acetyl-D-galactosamino-1,5-lactone [CPD:C22489];
N-acetyl-D-galactosaminate [CPD:C03408]
Comment
The enzyme, found in bacteria, also acts more slowly on N-acetyl-D-mannosamine.
History
EC 1.1.3.29 created 1992, modified 2022
Reference
1
  Authors
Horiuchi, T.
  Title
Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp. 15-1.
  Journal
Agric Biol Chem 53:361-368 (1989)
Reference
2  [PMID:34709726]
  Authors
Rembeza E, Boverio A, Fraaije MW, Engqvist MKM.
  Title
Discovery of Two Novel Oxidases Using a High-Throughput Activity Screen.
  Journal
Chembiochem 23:e202100510 (2022)
DOI:10.1002/cbic.202100510
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.29
IUBMB Enzyme Nomenclature: 1.1.3.29
ExPASy - ENZYME nomenclature database: 1.1.3.29
BRENDA, the Enzyme Database: 1.1.3.29
CAS: 121479-58-1

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