KEGG   ENZYME: 1.1.3.43
Entry
EC 1.1.3.43                 Enzyme                                 

Name
paromamine 6'-oxidase;
btrQ (gene name);
neoG (gene name);
kanI (gene name);
tacB (gene name);
neoQ (obsolete gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
paromamine:oxygen 6'-oxidoreductase
Reaction(IUBMB)
paromamine + O2 = 6'-dehydroparomamine + H2O2 [RN:R08896]
Reaction(KEGG)
R08896
Substrate
paromamine [CPD:C01743];
O2 [CPD:C00007]
Product
6'-dehydroparomamine [CPD:C17583];
H2O2 [CPD:C00027]
Comment
Contains FAD. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Works in combination with EC 2.6.1.93, neamine transaminase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces fradiae also catalyses EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase.
History
EC 1.1.3.43 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13552  paromamine 6'-oxidase
K20565  paromamine 6'-oxidase / 6'''-hydroxyneomycin C oxidase
K20566  paromamine 6'-oxidase / 2'-deamino-2'-hydroxyparomamine 6'-oxidase
K20577  paromamine/lividamine 6'-oxidase
Genes
SRN: A4G23_05124(neoG)
SKA: CP970_06660
Reference
1  [PMID:17206729]
  Authors
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB
  Title
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin.
  Journal
Chembiochem 8:283-8 (2007)
DOI:10.1002/cbic.200600371
Reference
2  [PMID:18408324]
  Authors
Yu Y, Hou X, Ni X, Xia H
  Title
Biosynthesis of 3'-deoxy-carbamoylkanamycin C in a Streptomyces tenebrarius mutant strain by tacB gene disruption.
  Journal
J Antibiot (Tokyo) 61:63-9 (2008)
DOI:10.1038/ja.2008.111
Reference
3  [PMID:21689223]
  Authors
Clausnitzer D, Piepersberg W, Wehmeier UF
  Title
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.
  Journal
J Appl Microbiol 111:642-51 (2011)
DOI:10.1111/j.1365-2672.2011.05082.x
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.43
IUBMB Enzyme Nomenclature: 1.1.3.43
ExPASy - ENZYME nomenclature database: 1.1.3.43
BRENDA, the Enzyme Database: 1.1.3.43

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