KEGG   ENZYME: 1.13.11.51
Entry
EC 1.13.11.51               Enzyme                                 
Name
9-cis-epoxycarotenoid dioxygenase;
nine-cis-epoxycarotenoid dioxygenase;
NCED;
AtNCED3;
PvNCED1;
VP14
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
9-cis-epoxycarotenoid 11,12-dioxygenase
Reaction(IUBMB)
(1) a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal [RN:R09682];
(2) 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al [RN:R06953];
(3) 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al [RN:R06952]
Reaction(KEGG)
Substrate
9-cis-epoxycarotenoid [CPD:C19811];
O2 [CPD:C00007];
9-cis-violaxanthin [CPD:C13433];
9'-cis-neoxanthin [CPD:C13431]
Product
2-cis,4-trans-xanthoxin [CPD:C13453];
12'-apo-carotenal [CPD:C19812];
(3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al [CPD:C14045];
(3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al
Comment
Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].
History
EC 1.13.11.51 created 2005
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09840  9-cis-epoxycarotenoid dioxygenase
Genes
AJM119058091
ATHAT1G30100(NCED5) AT1G78390(NCED9) AT3G14440(NCED3) AT3G24220(NCED6) AT4G18350(NCED2) AT4G19170(NCED4)
ALY9299069 9304023 9304074 9319543 9321111 9329661
CRB17877539 17879010 17892380 17893164 17895008 17898403
CSAT104702627 104713522 104718398 104718488 104723131 104723233 104731647 104741600 104745720 104746918 104751736 104757352 104765261 104766316 104777036 104779242 104792977
EUSEUTSA_v10002459mg EUTSA_v10007160mg EUTSA_v10019707mg EUTSA_v10020337mg EUTSA_v10024711mg EUTSA_v10027182mg
BRP103828685 103832319 103834110 103840429 103842019 103853125 103856252 103857159 103859576 103860984 103870025
BNA106348955 106354557 106354700 106360920 106360921 106363948 106367364 106370541 106373153 106374342 106377068 106387066 106394225 106397892 106409692 106422159 106422225 106423568 106432692 106432936 106439368 106441091 106449275 106454476 106454477 111200968 111208279 111212385
BOE106293405 106296147 106296637 106299768 106304727 106305032 106307955 106326515 106329010 106332377
RSZ108812568 108818727 108827163 108832505 108833968 108833987 108835472 108852895 108856254 108860168 108863091
THJ104806131 104806971 104808725 104810410 104812288 104814303 104820653 104822605
CPAP110811425 110812553 110826324
CIT102578064(NCED) 102578070 102612016 102612995 102621234
CICCICLE_v10006710mg CICLE_v10011335mg CICLE_v10013726mg CICLE_v10014639mg CICLE_v10019364mg CICLE_v10028113mg CICLE_v10030384mg CICLE_v10031003mg
PVY116105184 116106273 116130400 116144404
MINC123192534 123202160 123212226 123218366 123224367 123225824
TCC18590174 18590176 18594534 18595603 18596487 18602784 18606899 18606900 18606908
GRA105764938 105771210 105774706 105775929 105778279 105784100 105792802 105799279 105800347
GHI107890583 107901883 107905887 107909170 107912930 107913606 107914792 107918579 107928268 107936154 107936185 107942317 107942497 107946820 107947364
GAB108452385 108455532 108456360 108463293 108475519 108477078 108481073 108485604 108488699
DZI111276007 111278627 111280188 111280882 111284268 111287483 111291146 111300218 111302054 111318664
EGR104417139 104442316 104442535 104444180 104448637 104450035
GMX100777875 100780284 100797882(NCED2) 100814399
GSJ114388198 114412766 114418806 114422164 114422791
PVUPHAVU_002G120600g PHAVU_005G051600g PHAVU_007G198800g
VRA106759781 106770528 106777861
VAR108325341 108333042 108336017
VUN114174016 114190887 114191515
CCAJ109789738 109792298 109806734
APRC113854419 113855143 113868483
MTRMTR_2g070460 MTR_5g025230 MTR_5g025250 MTR_5g025270
CAM101492033 101496280 101505040 101511615
LJALj4g3v2467280.1(Lj4g3v2467280.1)
ADU107459843 107482022 107487371
AIP107613693 107632055 107642493
AHF112703460 112734761 112747607 112771106 112792524 112800212
LANG109333123 109334878 109348052 109359536 109361488
FVE101292752 101293791 101311757 101315210
RCN112164558 112168397 112197186 112198544
PPER18780686 18780753 18792568 18792639
PMUM103321735 103323745 103325135 103325828
PAVI110750920 110753229 110772343 110773976
PDUL117614927 117615268 117625163 117625951
MDM103403002 103420192 103431063 103435001 103435153 103445724 114820549
PXB103938475 103945979 103951286 103953513 103954827 103954831 103955326 103955327 103957334 103965230
ZJU107406906 107409395 107422564 107422565 107427259
MNT21400902 21404497 21405962 21407351
CSV101204647 101214535(NCED1) 101219505(NCED2) 101220226 101221572
CMO103484361(NCED3) 103489449 103493037 103499487 103503120(NCED2)
BHJ120067968 120070234 120074773 120082781 120085200
MCHA111012881
CMAX111478741 111479509 111479551 111483168 111483246 111495550 111497274 111499033
CMOS111433338 111433499 111434081 111437497 111437641 111447037 111448011 111460311
CPEP111781088 111783358 111792392 111793376 111803113 111807437 111809781 111810736
RCU8264093 8265632 8280147 8287688
JCU105634001 105643751 105650387
HBR110633552 110638127 110641806 110650899 110654913 110672115
MESC110600884 110601087 110601295 110602073 110604978 110610850 110612235 110629890 122721347 122724448
POP112327632 18095516 18102197 18103239 7460121 7472358 7478609 7493195 7494613 7497045
PEU105109608 105115643 105116862 105122973 105129260 105130411 105139409
PALZ118030894 118038221 118038286 118045555 118054538 118056662
JRE108986427 108988447 108990195 108990951 108994430 108995964
QSU111992501 111992711 111993152 112006543 112012911
QLO115971405 115987939 115992594
TWL119985327 119989589 119995140 119995378 120014446 120015931 120016039
VVI100232942(NCED1) 100232943(NCED2) 100244362 100251100(CCD4a) 100252859(CCD4b) 100254497
VRI117905473 117914078 117925641 117928669 117932651 117934177
SLY100316877 101246826 101250535 101250828 544163(NCED1)
SPEN107019771 107024003 107027314 107027395 107027835 107029051
SOT102577733 102577783(nced1) 102579540 102581808 102589614
CANN107846808 107848895 107852027 107870081 107872667
NTA107767418 107782076 107791323(CCD4) 107799511(NCED3) 107801738 107814412 107815312 107822968(NCED3-2) 107823578(CCD4) 107827644(NCED3)
NSY104228039 104232006 104235977 104244254 104249614 104249679
NTO104098488 104102138 104104691 104108198 104114696
NAU109206102 109208027 109209941 109215591 109231795 109243862
INI109150339 109153066 109153797 109156188(NCED1) 109165558 109176492 109188196
ITR115995609 116010384 116014264 116015544 116015747 116027424 116031448
SIND105155486 105155487 105155499 105156900 105161870 105165104 105165510 105172767 105175729 105176883
OEU111370875 111376755 111386468 111386602 111389266 111397100 111402235 111407264
EGT105948602 105948640 105950719 105957101 105957865 105957866 105957949 105962473 105962860 105966981 105966982 105966984 105966985 105967147 105970458 105970629 105971231 105971233 105971302
SSPL121750152 121750791 121751800 121754117 121759485 121763611 121766599 121766760 121767734 121770703 121773322 121775921 121778286 121778377 121783261 121784400 121788005
HAN110873138 110878258 110885603 110889755 110898252 110911735 110912650 110915557 110927366 110937034
ECAD122578151 122584949 122584999 122593174 122594426 122595814 122596373 122596544 122599400 122599932 122609680
LSV111879595 111880483 111880794 111881134 111889750 111900015 111906500 111912461
CCAV112500826 112504501 112504502 112506949 112516278 112516882 112517233 112520878 112521183 112525058 112526615 112528101
DCR108196216 108198628 108203135 108208230 108216136 108220121 108220601 108221345 108226639
CSIN114282383 114282858 114286258 114292414 114301623 114323809 114323833
BVG104883854 104893046 104902774 104907355
SOE110791327 110791467 110798853 110805680
CQI110686562 110692067 110728550 110729235 110730096
NNU104587905 104590616 104590705 104593669 104602221 104610284 104610285
MING122059411 122060240 122072123 122072837 122075667 122090577 122091177 122091560
TSS122639150 122641161 122649852 122655474 122668047 122668048 122668078
PSOM113275558 113288302 113301054
NCOL116253031 116265233 116265235 116265236 116265237 116265238 116265389 116266144 116266175 116267750
OSA4330451 4333566 4342424 4352125 9270250
DOSAOs02t0704000-01(Os02g0704000) Os03t0645900-00(Os03g0645900) Os07t0154100-01(Os07g0154100) Os12t0435200-01(Os12g0435200)
OBR102709247 102709942 102710691 102717690 102719350 102719521
BDI100822305 100835478 100836107 100845393
ATS109742916 109749210 109751445 109770448 109770578 109784162
TDC119298911 119303012 119307199 119311686 119317566 119320387 119357367
TAES123041264 123048173 123055773 123104904 123110233 123113194 123119285 123122716 123128116 123137906 123145208
TUA125507669 125507698 125510509 125514277 125536833
SBI110434687 8062208 8081132
ZMA100281136 100281391 100501444 100501454 103626043 103627610 103634160 103634451 103648268 103652265 732819
SITA101766978 101770668 101778945 101783411
SVS117834674 117836229 117845465 117847658
PVIR120649390 120650247 120656942 120659353 120662057 120668058 120692443 120700158
PHAI112877956 112881744 112884202 112885643 112900215
PDA103705744 103712994 103717739 103721240
EGU105036563 105037046 105046802 105059614
MUS103975672 103982044 103982259 103982953 103983524 103985103 103989554 103990944 103994222
DCT110097303 110103406 110114619
PEQ110033126 110036210 110039159
AOF109826914 109837791
ATR18422574 18427334 18444165
SMOSELMODRAFT_11287 SELMODRAFT_11289 SELMODRAFT_11292 SELMODRAFT_11293 SELMODRAFT_11304 SELMODRAFT_113788(NCED-1) SELMODRAFT_116420(NCED-2) SELMODRAFT_31991 SELMODRAFT_411585 SELMODRAFT_75383 SELMODRAFT_79628 SELMODRAFT_80651 SELMODRAFT_91815 SELMODRAFT_94523
PPP112277457 112293041
EHXEMIHUDRAFT_195386
 » show all
Reference
1  [PMID:9188535]
  Authors
Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR.
  Title
Specific oxidative cleavage of carotenoids by VP14 of maize.
  Journal
Science 276:1872-4 (1997)
DOI:10.1126/science.276.5320.1872
  Sequence
[zma:732819]
Reference
2  [PMID:9342392]
  Authors
Tan BC, Schwartz SH, Zeevaart JA, McCarty DR
  Title
Genetic control of abscisic acid biosynthesis in maize.
  Journal
Proc Natl Acad Sci U S A 94:12235-40 (1997)
DOI:10.1073/pnas.94.22.12235
  Sequence
[zma:732819]
Reference
3  [PMID:10611388]
  Authors
Qin X, Zeevaart JA
  Title
The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.
  Journal
Proc Natl Acad Sci U S A 96:15354-61 (1999)
DOI:10.1073/pnas.96.26.15354
  Sequence
Reference
4  [PMID:10929129]
  Authors
Thompson AJ, Jackson AC, Symonds RC, Mulholland BJ, Dadswell AR, Blake PS, Burbidge A, Taylor IB.
  Title
Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.
  Journal
Plant J 23:363-74 (2000)
DOI:10.1046/j.1365-313x.2000.00789.x
Reference
5  [PMID:11532178]
  Authors
Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K
  Title
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
  Journal
Plant J 27:325-33 (2001)
DOI:10.1046/j.1365-313x.2001.01096.x
  Sequence
[ath:AT3G14440]
Reference
6
  Authors
Iuchi, S., Kobayashi, M., Taji, T., Naramoto, M., Seki, M., Kato, T., Tabata, S., Kakubari, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K.
  Title
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
  Journal
Plant J 30:611 (2002)
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.51
IUBMB Enzyme Nomenclature: 1.13.11.51
ExPASy - ENZYME nomenclature database: 1.13.11.51
BRENDA, the Enzyme Database: 1.13.11.51
CAS: 199877-10-6

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