KEGG   ENZYME: 1.14.11.35
Entry
EC 1.14.11.35               Enzyme                                 
Name
1-deoxypentalenic acid 11beta-hydroxylase;
ptlH (gene name);
sav2991 (gene name);
pntH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
1-deoxypentalenate + 2-oxoglutarate + O2 = 1-deoxy-11beta-hydroxypentalenate + succinate + CO2 [RN:R10157]
Reaction(KEGG)
R10157
Substrate
1-deoxypentalenate [CPD:C20404];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
1-deoxy-11beta-hydroxypentalenate [CPD:C20403];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
History
EC 1.14.11.35 created 2012
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K18056  1-deoxypentalenic acid 11beta-hydroxylase
Genes
ADYHLG70_10405
SMASAVERM_2991(ptlH)
SBHSBI_09673
SCIB446_29665
SCWTU94_27375
SLEsle_13220(sle_13220)
SSIAA7J05_09270
SGJIAG43_27125
SHKJ2N69_35835
DSNHWI92_19215
Reference
1  [PMID:16704250]
  Authors
You Z, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:6566-7 (2006)
DOI:10.1021/ja061469i
  Sequence
Reference
2  [PMID:17942405]
  Authors
You Z, Omura S, Ikeda H, Cane DE, Jogl G
  Title
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis.
  Journal
J Biol Chem 282:36552-60 (2007)
DOI:10.1074/jbc.M706358200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.35
IUBMB Enzyme Nomenclature: 1.14.11.35
ExPASy - ENZYME nomenclature database: 1.14.11.35
BRENDA, the Enzyme Database: 1.14.11.35

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