KEGG ENZYME: 1.14.12.12

KEGG

ENZYME: 1.14.12.12

Entry

EC 1.14.12.12               Enzyme

Name

naphthalene 1,2-dioxygenase;
naphthalene dioxygenase;
naphthalene oxygenase;
NDO

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

naphthalene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Reaction(IUBMB)

naphthalene + NADH + H+ + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ [RN:R02968]

Reaction(KEGG)

R02968;
(other) R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R09159

Substrate

naphthalene [CPD:C00829];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

(1R,2S)-1,2-dihydronaphthalene-1,2-diol [CPD:C04314];
NAD+ [CPD:C00003]

Comment

This enzyme is a member of the ring-hydroxylating dioxygenase (RHD) family of bacterial enzymes that play a critical role in the degradation of aromatic compounds, such as polycyclic aromatic hydrocarbons [5]. This enzyme comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.3, ferredoxin---NAD+ reductase), an iron-sulfur oxygenase, and ferredoxin. Requires Fe2+.

History

EC 1.14.12.12 created 1992

Pathway

Polycyclic aromatic hydrocarbon degradation

Naphthalene degradation

Ethylbenzene degradation

Metabolic pathways

Microbial metabolism in diverse environments

Orthology

naphthalene 1,2-dioxygenase subunit alpha

naphthalene 1,2-dioxygenase subunit beta

Genes

PPI:	ND055(nahAc) ND056(nahAd)

PSC:	A458_06720 A458_06725

UIB01_22625 UIB01_22630

PBM:	CL52_14610 CL52_14615

PFK:	PFAS1_28610 PFAS1_28615

ALT:	ambt_17890 ambt_17895

RMN:	TK49_07955

BMK:	DM80_1443(ndoB) DM80_1444(ndoC)

CJU94_38655 CJU94_38660

PNA:	Pnap_2484 Pnap_2485

AJS:	Ajs_3092 Ajs_3093

Reference

1 [PMID:6874638]

Authors

Ensley BD, Gibson DT.

Title

Naphthalene dioxygenase: purification and properties of a terminal oxygenase component.

Journal

J Bacteriol 155:505-11 (1983)
DOI:10.1128/JB.155.2.505-511.1983

Reference

2 [PMID:234247]

Authors

Jeffrey AM, Yeh HJ, Jerina DM, Patel TR, Davey JF, Gibson DT.

Title

Initial reactions in the oxidation of naphthalene by Pseudomonas putida.

Journal

Biochemistry 14:575-84 (1975)
DOI:10.1021/bi00674a018

Reference

3 [PMID:9634695]

Authors

Kauppi B, Lee K, Carredano E, Parales RE, Gibson DT, Eklund H, Ramaswamy S

Title

Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.

Journal

Structure 6:571-86 (1998)
DOI:10.1016/S0969-2126(98)00059-8

Reference

4 [PMID:10692370]

Authors

Parales RE, Lee K, Resnick SM, Jiang H, Lessner DJ, Gibson DT

Title

Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

Journal

J Bacteriol 182:1641-9 (2000)
DOI:10.1128/JB.182.6.1641-1649.2000

Reference

5 [PMID:17014090]

Authors

Jouanneau Y, Meyer C, Jakoncic J, Stojanoff V, Gaillard J

Title

Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons.

Journal

Biochemistry 45:12380-91 (2006)
DOI:10.1021/bi0611311

Other DBs

ExplorEnz - The Enzyme Database:

IUBMB Enzyme Nomenclature:

ExPASy - ENZYME nomenclature database:

UM-BBD (Biocatalysis/Biodegradation Database):

BRENDA, the Enzyme Database:

CAS:

9074-04-8

DBGET integrated database retrieval system