Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy

naphthalene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

naphthalene + NADH + H+ + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ [RN:R02968]

R02968;
(other) R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R09159
Reaction

naphthalene [CPD:C00829];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

This enzyme is a member of the ring-hydroxylating dioxygenase (RHD) family of bacterial enzymes that play a critical role in the degradation of aromatic compounds, such as polycyclic aromatic hydrocarbons [5]. This enzyme comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.3, ferredoxin---NAD+ reductase), an iron-sulfur oxygenase, and ferredoxin. Requires Fe2+.

EC 1.14.12.12 created 1992

1  [PMID:6874638]

Ensley BD, Gibson DT.

Naphthalene dioxygenase: purification and properties of a terminal oxygenase component.

J Bacteriol 155:505-11 (1983)
DOI:10.1128/JB.155.2.505-511.1983

2  [PMID:234247]

Jeffrey AM, Yeh HJ, Jerina DM, Patel TR, Davey JF, Gibson DT.

Initial reactions in the oxidation of naphthalene by Pseudomonas putida.

Biochemistry 14:575-84 (1975)
DOI:10.1021/bi00674a018

3  [PMID:9634695]

Kauppi B, Lee K, Carredano E, Parales RE, Gibson DT, Eklund H, Ramaswamy S

Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.

Structure 6:571-86 (1998)
DOI:10.1016/S0969-2126(98)00059-8

4  [PMID:10692370]

Parales RE, Lee K, Resnick SM, Jiang H, Lessner DJ, Gibson DT

Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

J Bacteriol 182:1641-9 (2000)
DOI:10.1128/JB.182.6.1641-1649.2000

5  [PMID:17014090]

Jouanneau Y, Meyer C, Jakoncic J, Stojanoff V, Gaillard J

Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons.

Biochemistry 45:12380-91 (2006)
DOI:10.1021/bi0611311