KEGG   ENZYME: 1.14.12.24
Entry
EC 1.14.12.24               Enzyme                                 
Name
2,4-dinitrotoluene dioxygenase;
dntA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing)
Reaction(IUBMB)
2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+ [RN:R09233]
Reaction(KEGG)
R09233
Substrate
2,4-dinitrotoluene [CPD:C11006];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
4-methyl-5-nitrocatechol [CPD:C18315];
nitrite [CPD:C00088];
NAD+ [CPD:C00003]
Comment
This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.
History
EC 1.14.12.24 created 2015
Pathway
ec00633  Nitrotoluene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K14579  naphthalene 1,2-dioxygenase subunit alpha
K14580  naphthalene 1,2-dioxygenase subunit beta
Genes
PPIND055(nahAc) ND056(nahAd)
PSCA458_06720 A458_06725
PSTUUIB01_22625 UIB01_22630
PBMCL52_14610 CL52_14615
PFKPFAS1_28610 PFAS1_28615
ALTambt_17890 ambt_17895
RMNTK49_07955
BMKDM80_1443(ndoB) DM80_1444(ndoC)
PARBCJU94_38655 CJU94_38660
PNAPnap_2484 Pnap_2485
AJSAjs_3092 Ajs_3093
 » show all
Reference
1  [PMID:8759857]
  Authors
Suen WC, Haigler BE, Spain JC
  Title
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
  Journal
J Bacteriol 178:4926-34 (1996)
DOI:10.1128/JB.178.16.4926-4934.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.24
IUBMB Enzyme Nomenclature: 1.14.12.24
ExPASy - ENZYME nomenclature database: 1.14.12.24
BRENDA, the Enzyme Database: 1.14.12.24

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