ndmA (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
caffeine:oxygen oxidoreductase (N1-demethylating)
(1) caffeine + O2 + NAD(P)H + H+ = theobromine + NAD(P)+ + H2O + formaldehyde [RN:
(2) theophylline + O2 + NAD(P)H + H+ = 3-methylxanthine + NAD(P)+ + H2O + formaldehyde [RN:
(3) paraxanthine + O2 + NAD(P)H + H+ = 7-methylxanthine + NAD(P)+ + H2O + formaldehyde [RN:
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC
, methylxanthine N3-demethylase, and has a 5-fold higher activity with NADH than with NADPH . Also demethylate 1-methylxantine with lower efficiency. Forms part of the degradation pathway of methylxanthines.
EC 188.8.131.52 created 2013
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
K21722 methylxanthine N1-demethylase
Summers RM, Louie TM, Yu CL, Subramanian M
Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source.
Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M
Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids.