ndmB (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
theobromine:oxygen oxidoreductase (N3-demethylating)
(1) theobromine + O2 + NAD(P)H + H+ = 7-methylxanthine + NAD(P)+ + H2O + formaldehyde [RN:
(2) 3-methylxanthine + O2 + NAD(P)H + H+ = xanthine + NAD(P)+ + H2O + formaldehyde [RN:
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC
, methylxanthine N1-demethylase, and has higher activity with NADH than with NADPH . Also demethylates caffeine and theophylline with lower efficiency. Forms part of the degradation pathway of methylxanthines.
EC 220.127.116.11 created 2013
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
K21723 methylxanthine N3-demethylase
Summers RM, Louie TM, Yu CL, Subramanian M
Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source.
Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M
Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids.