KEGG   ENZYME: 1.14.13.208
Entry
EC 1.14.13.208              Enzyme                                 
Name
benzoyl-CoA 2,3-epoxidase;
benzoyl-CoA dioxygenase/reductase (incorrect);
BoxBA;
BoxA/BoxB system;
benzoyl-CoA 2,3-dioxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
benzoyl-CoA,NADPH:oxygen oxidoreductase (2,3-epoxydizing)
Reaction(IUBMB)
benzoyl-CoA + NADPH + H+ + O2 = 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O [RN:R09555]
Reaction(KEGG)
R09555
Substrate
benzoyl-CoA [CPD:C00512];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,3-epoxy-2,3-dihydrobenzoyl-CoA [CPD:C19684];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii. BoxB functions as the oxygenase part of benzoyl-CoA oxygenase in conjunction with BoxA, the reductase component, which upon binding of benzoyl-CoA, transfers two electrons to the ring in the course of monooxygenation. BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein (FAD), BoxB is a monomeric iron-protein [1].
History
EC 1.14.13.208 created 2010 as EC 1.14.12.21, transferred 2015 to EC 1.14.13.208
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K15511  benzoyl-CoA 2,3-epoxidase subunit A
K15512  benzoyl-CoA 2,3-epoxidase subunit B
Genes
XALXALC_1517(albXII)
THISHZT40_02635(boxA) HZT40_02640(boxB)
ADIB5T_02165 B5T_02166
AXEP40_10385 P40_10390
MARLHH196_07250(boxB) HH196_07255(boxA)
VFFVITFI_CDS1569 VITFI_CDS1589 VITFI_CDS1590
RPJN234_07040 N234_07045 N234_31275 N234_31280
REHH16_A1408(bdxA) H16_A1409(bdxB) H16_B1912(boxA) H16_B1913(boxB)
CNCCNE_1c14360(boxA) CNE_1c14370(boxB) CNE_2c18730(boxA) CNE_2c18740(boxB) CNE_BB1p04280(boxA)
CUHBJN34_07180 BJN34_07185 BJN34_32090 BJN34_32095
REUReut_A1323 Reut_A1324
RMERmet_1220(boxA) Rmet_1221(boxB)
CTIRALTA_A1321(bdxA) RALTA_A1322(bdxB) RALTA_B1611(boxA) RALTA_B1612(boxB)
CBWRR42_m1517 RR42_m1518 RR42_s3010 RR42_s3011
CGDCR3_3379(boxB) CR3_3380(boxA)
CCUPBKK81_26785 BKK81_26790
CUPBKK80_29525 BKK80_29530
CUUBKK79_35480 BKK79_35485
CPAUEHF44_15400(boxB) EHF44_15405(boxA)
COXE0W60_03165(boxA) E0W60_03170(boxB) E0W60_16990(boxA) E0W60_16995(boxB)
CCAMM5D45_06150(boxA) M5D45_06155(boxB)
BUGBC1001_2389 BC1001_2390
BGFBC1003_1068 BC1003_1069
BUQAC233_12235 AC233_12240
BXEBxe_A1423(boxB) Bxe_C0890(boxA) Bxe_C0891(boxB)
BXBDR64_3587(boxB) DR64_3588(boxA) DR64_7559(boxB) DR64_7560(boxA)
BPHBphy_1538 Bphy_1539
BGEBC1002_1975 BC1002_1976
BPXBUPH_02965 BUPH_02966
BPYBphyt_2694 Bphyt_2695
BUZAYM40_13840 AYM40_13845
BFNOI25_2716(boxB) OI25_2717(boxA)
BCAIK788_0006465 K788_0006466
PSPWBJG93_02490(boxB) BJG93_02495(boxA)
PARBCJU94_11400(boxB) CJU94_11405(boxA)
PHSC2L64_05440(boxB) C2L64_05445(boxA)
PTERC2L65_05225(boxB) C2L65_05230(boxA)
PGPCUJ91_12135(boxA) CUJ91_12140(boxB)
PCJCUJ87_05135(boxA)
PTSCUJ90_12200(boxA) CUJ90_12205(boxB)
PMEGFNZ07_26470(boxA) FNZ07_26475(boxB)
PTROG5S35_23230(boxB) G5S35_23235(boxA)
PGISI6I06_01100(boxB) I6I06_01105(boxA)
PACPFAZ97_21015(boxA) FAZ97_21020(boxB)
PACSFAZ98_17215(boxA) FAZ98_17220(boxB)
PDIOPDMSB3_3010(boxA) PDMSB3_3011(boxB)
BRHRBRH_00498 RBRH_00499
PPKU875_06785 U875_06790
PPNODA70_23860 DA70_23865
PPNMLV28_06845 LV28_06850
PRBX636_02785 X636_02790
PPULRO07_00930 RO07_00935
PSPUNA29_10730 NA29_10735
PAPISG18_01105 SG18_01110
PVEUC34_17185 UC34_17190
POXMB84_21960 MB84_21965
PTXABW99_07615 ABW99_07620
PFGAB870_04225 AB870_04230
PNRAT302_14910 AT302_14915
PANDDRB87_15890(boxB)
PFIBPI93_010795(boxB) PI93_010800(boxA)
PCOMNTU39_18885(boxA) NTU39_18890(boxB)
BPTBpet3568(petH) Bpet3569(pbcA)
BHOD560_2021(boxB) D560_2022(boxA)
BHMD558_2006(boxB) D558_2007(boxA)
BHZACR54_04083(boxA) ACR54_04084(boxB)
BBROBAU06_05180 BAU06_05185
BFZBAU07_21240 BAU07_21245
BPDZBBN53_02670 BBN53_02675
BGMCAL15_08655 CAL15_08660
AXYAXYL_05030(boxA) AXYL_05031(boxB)
AXONH44784_035141 NH44784_035151
AXNAX27061_4849 AX27061_4850
AXXERS451415_04886(boxA) ERS451415_04887(boxB)
ADTAPT56_22175 APT56_22180
AISBUW96_13975 BUW96_13980
ASWCVS48_13535(boxB) CVS48_13540(boxA)
ACHBDVB37_21270(boxA) DVB37_21275(boxB)
ADYHLG70_28660(boxB) HLG70_28665(boxA)
ACHOH4P35_20150(boxA) H4P35_20155(boxB)
PUSCKA81_01055(boxB) CKA81_01060(boxA)
PUDG9Q38_06775(boxB) G9Q38_06780(boxA)
AKATKWG_07590 TKWG_07595
AMIMMIM_c27160(boxA) MIM_c27170(boxB)
CDNBN940_14751 BN940_14756
PIGEGT29_17285(boxA) EGT29_17290(boxB)
PARKLSG25_10585(boxA) LSG25_10590(boxB)
RFRRfer_0221 Rfer_0222 Rfer_0228
RHFEUB48_04950(boxB) EUB48_04955(boxA)
POLBpro_1629 Bpro_1630
PNAPnap_2942 Pnap_2943
POSDT070_17675(boxB)
POOF7R28_12910(boxB)
PVACHC248_01624(boxB)
ACKC380_20165 C380_20170
VEIVeis_0733 Veis_0734
DACDaci_0072 Daci_0073
DELDelCs14_0073 DelCs14_0074
DTSBI380_22090 BI380_22095
DHKBO996_02630 BO996_02635
DLAI6G47_22040(boxA) I6G47_22045(boxB)
VAPVapar_0094 Vapar_0095
VPEVarpa_0110 Varpa_0111
VPDVAPA_1c00960(boxB) VAPA_1c00970(boxA)
VBOCKY39_00545(boxB) CKY39_00550(boxA)
VAMC4F17_18550(boxA) C4F17_18555(boxB)
CTTCtCNB1_0065 CtCNB1_0066
CTESO987_00305 O987_00310
RTARta_22300
OTDJ1M35_05285(boxB) J1M35_05290(boxA)
LIML103DPR2_00830(boxB) L103DPR2_00831(boxA)
LIHL63ED372_02245(boxA) L63ED372_02246(boxB)
HYRBSY239_635(boxB) BSY239_636(boxA)
HYBQ5W_16380 Q5W_16385
HYLLPB072_05890 LPB072_05895
HYCE5678_00695(boxB) E5678_00700(boxA)
HPSEHPF_06705(boxB) HPF_06710(boxA)
HYNF9K07_05880(boxB) F9K07_05885(boxA)
HTNKI616_00735(boxB) KI616_07625(boxB) KI616_07630(boxA) KI616_12830(boxB) KI616_12835(boxA)
HCZG9Q37_21450(boxB) G9Q37_21455(boxA)
DIHG7047_17820(boxB) G7047_17825(boxA)
DAERH9K75_07770(boxA)
DRGH9K76_04120(boxA) H9K76_04125(boxB)
CBAASRAA_0585(boxB) SRAA_0586(boxA)
SAQAOMP39_14385(boxB) OMP39_14390(boxA)
SNNEWH46_00460(boxB) EWH46_00465(boxA)
ATERMW290_08050(boxA) MW290_08055(boxB)
LCHLcho_3660 Lcho_3661
THITHI_0466(boxA) THI_0467(boxB)
SHDSUTH_01641 SUTH_01642(boxB)
EBAebA2765(boxB) ebA2766(boxA)
ABREpbN1_17410(boxB) pbN1_17420(boxA)
APETToN1_26650(boxA) ToN1_26660(boxB)
AZOazo3057(boxB) azo3058(boxA)
AOAdqs_3191 dqs_3192
AZAAZKH_4519(boxB) AZKH_4520(boXA)
AZIAzCIB_4637 AzCIB_4638
AZDCDA09_22740(boxB) CDA09_22745(boxA)
AZRCJ010_18910(boxB) CJ010_18915(boxA)
TMZTmz1t_3908
TCLTchl_1102 Tchl_1103
TAKTharo_1133 Tharo_1134
DDZDSYM_19390 DSYM_19400
APAUAMPC_37390
SURSTAUR_7626(boxB)
AVMJQX13_24540(boxB)
VINAKJ08_1016
SCLsce1060(yfcX)
SCUSCE1572_07645
CCROCMC5_025770 CMC5_025780
PAUUE8A73_043340(boxB)
SAMYDB32_002623
LLUAKJ09_05632
MRMA7982_06572
HOHHoch_5225
MESOBSQ44_18020 BSQ44_18030
RPODE0E05_11135(boxA) E0E05_11140(boxB)
NIYFQ775_02915(boxA) FQ775_02920(boxB)
KMNHW532_10735(boxB) HW532_10740(boxA)
ABAWD5400_06470(boxB) D5400_06475(boxA)
BJAblr1081(blr1081)
BJUBJ6T_11110
BJPRN69_05425
BRABRADO6788(boxB)
BBTBBta_0750(boxB) BBta_6638(boxA) BBta_6639(boxB)
BRSS23_67350
AOLS58_06720
BRCBCCGELA001_34210
BICLMTR13_36350
BROBRAD285_6936(boxB)
BRKCWS35_10185(boxB)
BOTCIT37_08870(boxB)
BRQCIT40_30010(boxB)
BGQX265_34635(boxB)
BGZXH91_33020(boxB)
BSYMCIT39_29125(boxB)
BBETF8237_13005(boxB)
BARHWN72_46140(boxB)
BVZBRAD3257_8645(boxB)
BELBE61_90290(boxB)
BDGLPJ38_02110(boxB)
BSEPHAP48_0011930(boxB)
BQBJ4P68_0015770(boxB)
BSEIKMZ68_03460(boxB) KMZ68_03465(boxA) KMZ68_03470(boxB)
RPARPA0677
RPBRPB_4653
RPDRPD_0881
RPERPE_0584
RPTRpal_0744
RPXRpdx1_4109
RBKE0H22_11695(boxB) E0H22_11700(boxA)
VGOGJW-30_1_01040(boxB) GJW-30_1_01041(boxA)
TRBHB776_18400(boxA) HB776_18405(boxB)
RHZRHPLAN_60810
PSINCAK95_09715 CAK95_09720
RBMTEF_07960
CAULKCG34_06220(boxB)
SILSPO3701(boxB) SPO3703(boxA)
JANJann_0672(boxB)
RPONG3256_16600(boxA) G3256_16605(boxB)
SUAMBOO69_15565 BOO69_15570
SULZC1J03_02545(boxB) C1J03_02555(boxA)
SMEDJNX03_11395(boxA) JNX03_11400(boxB)
SPSESULPSESMR1_02743(boxA) SULPSESMR1_02745(boxB)
TOMBWR18_14110
RIDRIdsm_00626(boxB) RIdsm_00628(boxA)
AHTANTHELSMS3_04341(boxA) ANTHELSMS3_04343(boxB)
SAGUCDO87_17735(boxB) CDO87_17745(boxA)
THAACFI11_22010(boxB)
OCDFHY55_19195(boxA) FHY55_19200(boxB)
MALUKU6B_38040 KU6B_38050
TGLHFZ77_06780(boxA) HFZ77_06790(boxB)
PZHCX676_12415(boxA)
PTPRCA23_c19060(boxA) RCA23_c19070(boxB)
SEDIEBB79_19920(boxA) EBB79_19925(boxB)
GCEKYE46_09685(boxB) KYE46_09695(boxA)
MAGamb0175 amb0176
MAGXXM1_1070(boxB) XM1_1071(boxA)
MAGNWV31_13525 WV31_13530
FERFNB15_18640(boxB)
NACINUH88_09980(boxB)
SNEANBZ79_15020(boxB)
MFJMFLOJ_46030
MMORMMOR_02900
GOQACH46_13370
GOILK459_15645(boxB)
TOYFO059_00815(boxB)
SBATG4Z16_02410(boxB)
JLIEXU32_11305(boxB)
NPSKRR39_16265(boxB)
SAIUJ4H86_15070(boxB)
NOABKM31_25130
NGNLCN96_23775(boxB)
BSDBLASA_2556(boxB)
AMDAMED_8610
AMNRAM_44190
AMMAMES_8479
AMZB737_8480
AOIAORI_7396
AJAAJAP_02020(boxB)
AMQAMETH_6479
AMYCCU254_35735(boxB)
AMYBBKN51_30385
AMYYYIM_21145(boxB)
AORISD37_03480
AACDLWP59_36010(boxB)
APRTMUY14_40655(boxB)
AROONQK81_28605(boxB)
PDXPsed_1489
PSEAWY02_22245
PSEEFRP1_20445
PSEHXF36_05450
PSEQAD006_27860
PECQAD017_07455
PHHAFB00_18000
PAUTPdca_13940
PBROHOP40_01905(boxB)
KPHYAOZ06_19790
PMADBAY61_05970
CAICaci_2007
EKEEK0264_08340(boxB)
 » show all
Reference
1  [PMID:15458418]
  Authors
Zaar A, Gescher J, Eisenreich W, Bacher A, Fuchs G
  Title
New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii.
  Journal
Mol Microbiol 54:223-38 (2004)
DOI:10.1111/j.1365-2958.2004.04263.x
  Sequence
Reference
2  [PMID:12399500]
  Authors
Gescher J, Zaar A, Mohamed M, Schagger H, Fuchs G
  Title
Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii.
  Journal
J Bacteriol 184:6301-15 (2002)
DOI:10.1128/JB.184.22.6301-6315.2002
  Sequence
Reference
3  [PMID:11222587]
  Authors
Mohamed ME, Zaar A, Ebenau-Jehle C, Fuchs G
  Title
Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii.
  Journal
J Bacteriol 183:1899-908 (2001)
DOI:10.1128/JB.183.6.1899-1908.2001
Reference
4  [PMID:20452977]
  Authors
Rather LJ, Knapp B, Haehnel W, Fuchs G
  Title
Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation.
  Journal
J Biol Chem 285:20615-24 (2010)
DOI:10.1074/jbc.M110.124156
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.208
IUBMB Enzyme Nomenclature: 1.14.13.208
ExPASy - ENZYME nomenclature database: 1.14.13.208
BRENDA, the Enzyme Database: 1.14.13.208

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