KEGG   ENZYME: 1.14.13.218
Entry
EC 1.14.13.218              Enzyme                                 
Name
5-methylphenazine-1-carboxylate 1-monooxygenase;
phzS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Reaction(IUBMB)
5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanin + NAD+ + CO2 + H2O [RN:R11346]
Reaction(KEGG)
R11346;
(other) R11578
Substrate
5-methylphenazine-1-carboxylate [CPD:C21295];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
pyocyanin [CPD:C01748];
NAD+ [CPD:C00003];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.
History
EC 1.14.13.218 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20940  5-methylphenazine-1-carboxylate 1-monooxygenase
Genes
RORRORB6_06595
RPLNB1209_13685
RAODSD31_13210
YREHEC60_05120
PSGCG163CM_02640(phzS)
EBFD782_1281
PAOPat9b_1855
LABLA76x_3342(phzS)
LAQGLA29479_2375(phzS)
PAEPA4217(phzS)
PAEVN297_4349
PAEIN296_4349
PAUPA14_09400(phzS)
PAPPSPA7_0881
PAGPLES_07101(phzS)
PAFPAM18_0720(phzS)
PNCNCGM2_5462(phzS)
PAEBNCGM1900_0742(phzS)
PDKPADK2_03585
PSGG655_03620
PRPM062_22220
PAEPPA1S_03735
PAERPA1R_gp2108
PAEMU769_03765
PAELT223_03665
PAESSCV20265_0744
PAEUBN889_04688(mhbM)
PAEGAI22_00175
PAECM802_4347
PAEOM801_4215
WOCBA177_13885
GAIIMCC3135_15925(xlnD)
MMEMarme_1535
MPCMar181_2201
RSCRCFBP_20884
RSLRPSI07_2763
RSNRSPO_c02774
RSYRSUY_26660
RPIRpic_0514
RPFRpic12D_0501
RPJN234_02860
RMNTK49_07090
RINACS15_0633
RSGJK151_05465
REHH16_A0578(h16_A0578)
CNCCNE_1c06050
CUHBJN34_03480
CTIRALTA_A0534
CBWRR42_m0670
CGDCR3_2101(ubiH)
CCUPBKK81_05525 BKK81_20090
CUPBKK80_04830 BKK80_21715
CUUBKK79_07450 BKK79_26880
CPAUEHF44_22900
COXE0W60_13510
BMABMAA0391
BMVBMASAVP1_1585
BMLBMA10229_1769
BMNBMA10247_A0439
BMALDM55_3356
BMAEDM78_4291
BMAQDM76_4054
BMAIDM57_4923
BMAFDM51_4113
BMAZBM44_3316
BMABBM45_4154
BPSBPSS2111
BPMBURPS1710b_A1214
BPLBURPS1106A_A2849
BPDBURPS668_A2974
BPSEBDL_5573
BPSMBBQ_4004(phzS)
BPSUBBN_5593(phzS)
BPSDBBX_4740(phzS)
BPZBP1026B_II2272
BPQBPC006_II2811
BPKBBK_4877(phzS)
BPSHDR55_5621
BPSABBU_3886(phzS)
BPSOX996_5401
BUTX994_4948
BSAVWS86_29635
BCENDM39_5111
BUZAYM40_21510
PPULRO07_14100
PSPUNA29_23010
PAPISG18_13925
PVEUC34_05365
POXMB84_09755
PFGAB870_16335
PNRAT302_01670
PFIBPI93_023035
BPTBpet0515 Bpet4332
BBROBAU06_23000
BFZBAU07_23145
BOHAKI39_21985
BGMCAL15_01535
ASWCVS48_28980
ACHBDVB37_05300
PUTPT7_0833
PUSCKA81_04285
PUDG9Q38_01140 G9Q38_02970 G9Q38_10125
PIGEGT29_07110 EGT29_22735 EGT29_28210
PARKLSG25_16400
RHFEUB48_12265
POLBpro_2199
AAVAave_0794
AAAAcav_0721
ACKC380_22685
ACRABSY15_1202
ACIOEAG14_01270
ARADKI609_21425
DACDaci_3698 Daci_4386
DELDelCs14_2432
DTSBI380_04640 BI380_07740
DHKBO996_16805 BO996_19770
DLAI6G47_02380 I6G47_05635
VAPVapar_5387
VPEVarpa_0285
VPDVAPA_1c03380
VBOCKY39_01595
VAMC4F17_17395
RTARta_23930
LIML103DPR2_01284(xlnD_2)
HYRBSY239_2040 BSY239_2625
HYBQ5W_04140
STHMIS481_16100
RDPRD2015_403
PKTAT984_17755
MIUABE85_21150
RGUA4W93_14405
THKCCZ27_01430
SCLsce4807 sce4897 sce7525
SCUSCE1572_07320 SCE1572_19460 SCE1572_24720 SCE1572_28650
MLNA9174_07680
MCIMesci_2836
MOPMesop_0529
MESWA9K65_015455
MESMEJ066_25025
MESPC1M53_21380
MESOBSQ44_20480
MESMeso_2927
HOEIMCC20628_02267
AAKAA2016_0349
NIYFQ775_05345 FQ775_14565
KMNHW532_11120
NGLRG1141_PA12940
NGGRG540_PA13930
NEOCYG48_01500
PDESFE840_017780
OANOant_3996
OAHDR92_4259
OCRHGK82_22735
OCLGTN27_15175
BJAbll2502(bll2502)
BJUBJ6T_36290 BJ6T_73250
BJPRN69_17595 RN69_35515
BRABRADO0592
BBTBBta_2684 BBta_7587
BRSS23_21960 S23_54840
AOLS58_52740 S58_70480
BRCBCCGELA001_25810 BCCGELA001_27265
BRADBF49_2290 BF49_5554
BICLMTR13_07380 LMTR13_29995
BROBRAD285_0059 BRAD285_5019
BRKCWS35_14465 CWS35_19400
BOTCIT37_00105 CIT37_23885
BRQCIT40_06980 CIT40_22375
BGQX265_12385 X265_28550
BGZXH91_11155 XH91_26510
BSYMCIT39_05770 CIT39_21570
BBETF8237_07380 F8237_26055
BARHWN72_12725 WN72_33960
BVZBRAD3257_1807 BRAD3257_3050 BRAD3257_6551
BELBE61_30130
BDGLPJ38_15290 LPJ38_32215
BSEPHAP48_0006725
BQBJ4P68_0029205
RPARPA3588
RPBRPB_1945
RPTRpal_4108
RPXRpdx1_1762
RBKE0H22_08280 E0H22_20405
VGOGJW-30_1_00854(xlnD_1)
TRBHB776_01665 HB776_28510
BOPAXW83_01690
BOSBSY19_1787
BVVBHK69_12440 BHK69_29720
BOIBLM15_12140 BLM15_12830
BOFFQV39_18055
LNEFZC33_04360
METM446_3637
MNOMnod_1206
MAQUMaq22A_c15030(ubiH)
MEEDA075_24315
MTEADK419_08600
MINDmvi_06480
CHELAL346_06160
RHZRHPLAN_29510 RHPLAN_38520 RHPLAN_41260 RHPLAN_49870
DEIC4375_06665
BRND1F64_21235
PSINCAK95_08390 CAK95_09835
NOHG5V57_23915
RBMTEF_16205
PPHRAPZ00_18290
SIWGH266_11530 GH266_16295
PHRC6569_06010 C6569_12535
PSTGE8M01_22865 E8M01_33480
SILSPO0105
SITTM1040_3441
PGAPGA1_c32020
PGLPGA2_c00680
PGDGal_00046
PHPPhaeoP97_00080
PPICPhaeoP14_00046
PHQD1820_13510
LMDMETH_18965
LEJETW24_18490
LAQUR2C4_19230
PABYGa0080574_TMP326
SUAMBOO69_14545
SULIC1J05_00215 C1J05_15695
SPOTG6548_14995
SMEDJNX03_12645
SPSESULPSESMR1_00290(hpxO)
RIDRIdsm_02011(xlnD)
AHTANTHELSMS3_04104
SAGUCDO87_15335
THAACFI11_23685
OCDFHY55_04475
ROTFIV09_19090(xlnD2)
MALUKU6B_16810
TGLHFZ77_15830
PARSDRW48_00150
PTPRCA23_c26230
RMBK529_017940
SALOEF888_02425
SEDIEBB79_00110
BOOE2K80_06690
PSEBEOK75_00130
PPRUFDP22_18945
PAMOBAR1_08670
POZI0K15_14380
PGVSL003B_3095
THASC6Y53_14570
HDHG5B40_10800
NARSaro_2238
NOTC7W88_14160
NDRHT578_01140
SWISwit_3061
SPHDHY78_10270
SECHB18_04230
ACRAcry_0321
AMVACMV_03520
ROSCTJ15_00570
RMUCFOB66_02020
TMOTMO_3126(mhbM)
FERFNB15_15520
APBSAR116_0061
BKWBkAM31D_08555(xlnD_1)
LAOAOX59_16860
BCHSJNE38_15130
MSAMycsm_03029
MYVG155_17420
MGOAFA91_20850
MDXBTO20_14290
MSENK3U95_14820
MSMMSMEG_3841
MSGMSMEI_3751
MSBLJ00_19080
MSNLI99_19085
MSHLI98_19090
MNED174_17475
MYNMyAD_17130
MFTXA26_35530
MAUUNCTC10437_03211(xlnD_1)
MHEVMHEL_55810
MAUBMAUB_10010
MPHUMPHO_03200
MMUCC1S78_016830
MMATMMAGJ_70400
MBOKMBOE_38750
MFGK6L26_14510
MSEIMSEDJ_44470
MABMAB_2237c
MMVMYCMA_1195
MABBMASS_2167
MABLMMASJCM_2192
MCHEBB28_11255
MIZBAB75_11975
MSAOMYCSP_09625
MSALDSM43276_01950(xlnD_2)
NCYNOCYR_0786
NNONONO_c08530
NAHF5544_05600 F5544_12955
NWLNWFMUON74_45100
SVLStrvi_5052
SHYSHJG_0206 SHJG_0216 SHJG_0800
SHOSHJGH_0633
SRCM271_47475
SMALSMALA_0139
SSOII1A49_04125
SCTSCAT_p0890
SCYSCATT_p08430
SBHSBI_01059
SVESVEN_5103
SGUSGLAU_04905
SVTSVTN_26265
SLDT261_1426 T261_7434
SLCSL103_08090
SXISXIM_24700 SXIM_24770
STRCAA958_29950 AA958_30740
SPAVSpa2297_23920 Spa2297_23950
SCLFBB341_29775
SALWCP975_12045
STRDNI25_24410
SNWBBN63_05605 BBN63_09595
SAUOBV401_04430
SALFSMD44_02958
SLXSLAV_02135
STROSTRMOE7_02870 STRMOE7_28970 STRMOE7_29000
SFKKY5_2781c
SLKSLUN_27535
SQZFQU76_24165 FQU76_30760
SKACP970_28680
SGZC0216_14055
SVNCP980_31315 CP980_32485
SFICEIZ62_09255
SPHVF9278_42105
STSUB7R87_23900 B7R87_26355
SGXH4W23_27835
SSPBCP982_16040 CP982_37385
SHUNDWB77_06919(xlnD)
SAUHSU9_000240
SMOBJ7W19_26555 J7W19_31275
SHKJ2N69_12195
KABB7C62_28850 B7C62_28880
YIALO772_03595
MICRBMW26_14970
MFOLDXT68_14210
MPRTET475_07395
MVDAWU67_12410 AWU67_14070
AGYATC03_03885
AARCG127AT_14310
LEULeucomu_09990
LTREVS81_13845
LLUTK1X41_09870
RTERIDM49_02300
DAYFV141_06960
LMOIVV02_01275
IDOI598_2461(hpxO_3)
CFLCfla_0298
CEZCBP52_12505
GYUFE374_11800
NDANdas_5023
SROSros_6172
NOABKM31_03465 BKM31_19720 BKM31_22685 BKM31_35635 BKM31_48365
NOWGBF35_47245
NCXNocox_08730(xlnD1)
NGNLCN96_09765 LCN96_24140 LCN96_38550
FREFranean1_5376
AMYCCU254_21595
AMYYYIM_22825(xlnD1)
AORISD37_26575
PDXPsed_3194
PSEEFRP1_21735
PSEQAD006_00910
PECQAD017_08735
PAUTPdca_23520
PMADBAY61_11550
MICBMicB006_2976
MTUACSH63_12410
ATLAthai_51270
ASERAsera_23600
CAICaci_4355
SNASnas_3295
AYMYM304_31330
DMRDeima_0219
PGINFRZ67_05915
HHYHalhy_0467
SLISlin_0336
SMONAWR27_00625
STAEHNV11_08740
RSIRunsl_0519
RUNDR864_05530
RUPDTQ70_10575
EOLEmtol_1587
 » show all
Reference
1  [PMID:11591691]
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4217]
Reference
2  [PMID:17253782]
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403
Reference
3  [PMID:18416536]
  Authors
Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF
  Title
Crystal structure of the pyocyanin biosynthetic protein PhzS.
  Journal
Biochemistry 47:5281-9 (2008)
DOI:10.1021/bi702480t
  Sequence
[pae:PA4217]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.218
IUBMB Enzyme Nomenclature: 1.14.13.218
ExPASy - ENZYME nomenclature database: 1.14.13.218
BRENDA, the Enzyme Database: 1.14.13.218

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