KEGG   ENZYME: 1.14.13.233
Entry
EC 1.14.13.233              Enzyme                                 
Name
4-hydroxy-6-methylpretetramide 12a-monooxygenase;
oxyL (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
4-hydroxy-6-methylpretetramide,NADPH:oxygen oxidoreductase (12a-hydroxylating)
Reaction(IUBMB)
4-hydroxy-6-methylpretetramide + NADPH + H+ + O2 = 4-de(dimethylamino)-4-oxoanhydrotetracycline + NADP+ + H2O [RN:R05462]
Reaction(KEGG)
R05462;
(other) R09190
Substrate
4-hydroxy-6-methylpretetramide [CPD:C06628];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-de(dimethylamino)-4-oxoanhydrotetracycline [CPD:C06627];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. The enzyme is bifunctional, and can also catalyse EC 1.14.13.232, 6-methylpretetramide 4-monooxygenase.
History
EC 1.14.13.233 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14252  6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
Genes
NAHF5544_10695
SHYSHJG_1193
SHOSHJGH_1028
SBHSBI_06866
SCIB446_13470
SPRISPRI_0201 SPRI_7152
SPAVSpa2297_32760
SGSAVL59_46185
SGMGCM10017557_73940
SPHWNFX46_25010
SGZC0216_31875
SVRCP971_24005
SGFHEP81_07938
SRIMCP984_02425
SPLACP981_34475
SHUNDWB77_05143(pcpB_4)
SXNIAG42_36430
SINEKI385_25240
SYANNRK68_34850
KAUB6264_18580
MIKFOE78_19175
AORISD37_34185
KALKALB_7834
ACTUActkin_02482(pcpB_1)
PLKCIK06_11100
DAURDaura_37895
CCAZCOUCH_15955
 » show all
Reference
1  [PMID:18422316]
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.233
IUBMB Enzyme Nomenclature: 1.14.13.233
ExPASy - ENZYME nomenclature database: 1.14.13.233
BRENDA, the Enzyme Database: 1.14.13.233

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