EC               Enzyme                                 

magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O (overall reaction) [RN:R10068];
(1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O [RN:R06265];
(1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O [RN:R06266];
(1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 3,8-divinyl protochlorophyllide a + NADP+ + 2 H2O [RN:R06267]
magnesium-protoporphyrin IX 13-monomethyl ester [CPD:C04536];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
3,8-divinyl protochlorophyllide a [CPD:C11831];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Requires Fe(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
EC created 2003, modified 2017
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
K04035  magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
ATH: AT3G56940(CRD1)
ALY: 9312457
CRB: 17887096
CSAT: 104712356 104781590 104791984
EUS: EUTSA_v10006009mg
BRP: 103830076 103841596
BNA: 106368040 106405693 106445907 111208747
BOE: 106299791 106312794
RSZ: 108817888 108862743
THJ: 104803235 104824384
CPAP: 110811309
CIT: 102612985
TCC: 18598690
GRA: 105786192
GAB: 108460633
EGR: 104443639
VRA: 106753941
VAR: 108329545
VUN: 114173988
CCAJ: 109802482
CAM: 101494559
LJA: Lj1g3v4058630.1(Lj1g3v4058630.1)
ADU: 107494229
AIP: 107604718
FVE: 101306835
RCN: 112193460
PPER: 18770050
PMUM: 103339106
PAVI: 110763662
MDM: 114822994
ZJU: 107423584
CSV: 101219179
CMO: 103502893
MCHA: 111021778
RCU: 8289525
JCU: 105648924
HBR: 110655527
MESC: 110623376
JRE: 108995356
QSU: 112034337
VVI: 100241461
SLY: 101257518
SPEN: 107002129
SOT: 102600334(at103)
CANN: 107844048
NSY: 104227011
NTO: 104089135
NAU: 109242165
INI: 109161721(PNIL34)
HAN: 110884853
LSV: 111879482
CCAV: 112518108
BVG: 104889639
SOE: 110780654
NNU: 104593560
OSA: 4326901
DOSA: Os01t0279100-01(Os01g0279100)
OBR: 102712668
BDI: 100828682
ATS: 109776579
SBI: 8074544
SITA: 101773118
PDA: 103698135
EGU: 105036097
DCT: 110113521
PEQ: 110028095
AOF: 109848727
ATR: 18426189
MNG: MNEG_2725
APRO: F751_5195
CME: CymeCp123(ycf59)
HYR: BSY239_1293(acsF)
METP: C1M51_03515(acsF)
RGE: RGE_33550(acsF)
METR: BSY238_2458(acsF)
RPOD: E0E05_11850(acsF)
AGC: BSY240_3656(acsF)
ABAW: D5400_04995(acsF)
BRA: BRADO1649(acsF)
BBT: BBta_6407(acsF)
BRS: S23_19480(acsF)
AOL: S58_58890
BRO: BRAD285_5752(acsF)
BRQ: CIT40_23790(acsF)
BGZ: XH91_02790(acsF)
BBET: F8237_24825(acsF)
RPA: RPA1552
RPB: RPB_3971
RPC: RPC_1323
RPD: RPD_3726
RPE: RPE_1357
RPT: Rpal_1740
BOS: BSY19_4045(acsF)
MEA: Mex_1p5263(acsF)
MDI: METDI5863(acsF)
MEX: Mext_4806
MCH: Mchl_5273
MPO: Mpop_5351
MET: M446_3728
MOR: MOC_2242(acsF)
META: Y590_24560
MEE: DA075_04100(acsF)
METD: C0214_26300(acsF)
METS: DK389_01020(acsF)
METI: DK427_17970(acsF)
MMES: MMSR116_22300(acsF)
MTEA: DK419_05060(acsF)
MSL: Msil_2032
BBAR: RHAL1_00849(acsF)
MROS: EHO51_17220(acsF)
RSP: RSP_0294
JAN: Jann_0151(acsF)
RDE: RD1_0129(acsF)
RLI: RLO149_p940810(acsF)
RPON: G3256_19035(acsF)
DSH: Dshi_3544(acsF)
PTP: RCA23_c29820(acsF)
RSU: NHU_00221(acsF)
RHC: RGUI_1977
ROM: EI983_05500(acsF)
RBG: BG454_02870(acsF)
THAA: CFI11_23890(acsF)
GEH: HYN69_05365(acsF)
TAW: EI545_18785(acsF)
MALU: KU6B_16560(acsF)
POZ: I0K15_16950(acsF)
FAQ: G5B39_10045(acsF)
NOT: C7W88_00610(acsF)
NGF: FRF71_09275(acsF)
NOG: GKE62_08565(acsF)
SPHI: TS85_22955
SSAN: NX02_18395
BLAS: BSY18_2698(acsF)
PALG: HFP57_06820(acsF)
PARJ: J4G78_07405(acsF)
SAND: H3309_10305(acsF)
ALH: G6N82_11565(acsF)
ERK: CD351_14000(acsF)
PHZ: CHX26_14625(acsF)
POT: E2E27_07210(acsF)
AMV: ACMV_19060(acsF)
NCB: C0V82_18105(acsF)
SKT: IGS68_17055(acsF)
RCE: RC1_2105(acsF)
SYN: sll1214 sll1874(AT103)
SYZ: MYO_116600(at103) MYO_150
SYY: SYNGTS_0005(sll1214) SYNGTS_1645(chlAII)
SYT: SYNGTI_0005(sll1214) SYNGTI_1645(chlAII)
SYS: SYNPCCN_0005(sll1214) SYNPCCN_1644(chlAII)
SYQ: SYNPCCP_0005(sll1214) SYNPCCP_1644(chlAII)
SYJ: D082_08250(AT103) D082_11950(ycf59)
SYO: C7I86_00025(acsF) C7I86_08535(acsF)
SYC: syc2188_d(ycf59)
SYG: sync_1304(acsF)
SYR: SynRCC307_1476(acsF)
SYX: SynWH7803_1102(acsF)
CYA: CYA_2703(acsF)
CYB: CYB_1254(acsF)
SYNR: KR49_02360
SYND: KR52_09945
SYNW: SynWH8103_01332(acsF)
SYNC: CB0101_00325(acsF) CB0101_01670(acsF)
TEL: tlr1426(ycf59) tlr1722
THN: NK55_02575(acsF1) NK55_05260(acsF2)
THEC: FFX45_07110(acsF) FFX45_08845(acsF)
CYI: CBM981_0231(acsF) CBM981_1951(acsF)
HHG: XM38_022230(acsF3)
PSER: ABRG53_0108(acsF) ABRG53_3949(acsF)
PMA: Pro_0992(acsF)
PMM: PMM0844(PNIL34)
PMT: PMT_2196
PMB: A9601_10251(pniL34)
PMC: P9515_09201(pnilL34)
PMF: P9303_29201(pniL34)
PMG: P9301_10241(pniL34)
PMH: P9215_10571(pniL34)
PMJ: P9211_09191(pniIL34)
PME: NATL1_08941(pnil34)
PRC: EW14_1059
PRM: EW15_0910
AMR: AM1_0465(acsF) AM1_2295(acsF)
THEU: HPC62_08165(acsF)
CYL: AA637_00120(acsF) AA637_14735(acsF-2)
ENN: FRE64_04905(acsF) FRE64_09065(acsF)
TER: Tery_0728
ARP: NIES39_B00770(acsF)
OXY: HCG48_01810(acsF) HCG48_10825(acsF)
LFS: HFV01_07170(acsF)
GVI: gvip493(ycf59)
GLJ: GKIL_2292(acsF)
NOE: CLI64_10670(acsF) CLI64_21635(acsF) CLI64_28005(acsF)
NED: HUN01_03560(acsF) HUN01_10900(acsF)
ANB: ANA_C10475(acsF) ANA_C11197(acsF)
ANN: EH233_00590(acsF) EH233_12300(acsF)
NSP: BMF81_00211(acsF) BMF81_04265(acsF_2)
DFS: HGD76_05435(acsF) HGD76_06245(acsF)
CCUR: IAR63_09695(acsF)
AFLO: HEQ12_04350(acsF) HEQ12_08955(acsF)
TOQ: HCG51_06915(acsF) HCG51_14440(acsF) HCG51_32660(acsF)
CTHE: Chro_1308
RCA: Rcas_1541
CAU: Caur_2590
CAG: Cagg_1285
 » show all
1  [PMID:3202840]
Walker CJ, Mansfield KE, Rezzano IN, Hanamoto CM, Smith KM, Castelfranco PA
The magnesium-protoporphyrin IX (oxidative) cyclase system. Studies on the mechanism and specificity of the reaction sequence.
Biochem J 255:685-92 (1988)
2  [PMID:12226378]
Bollivar DW, Beale SI.
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase (Characterization and Partial Purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803).
Plant Physiol 112:105-114 (1996)
3  [PMID:11790744]
Pinta V, Picaud M, Reiss-Husson F, Astier C
Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester.
J Bacteriol 184:746-53 (2002)
4  [PMID:14673103]
Tottey S, Block MA, Allen M, Westergren T, Albrieux C, Scheller HV, Merchant S, Jensen PE
Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.
Proc Natl Acad Sci U S A 100:16119-24 (2003)
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CAS: 92353-62-3

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