KEGG   ENZYME: 1.14.14.158
Entry
EC 1.14.14.158              Enzyme                                 

Name
carotenoid epsilon hydroxylase;
CYP97C1;
LUT1;
CYP97C;
carotene epsilon-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
alpha-carotene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
(1) alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2 = alpha-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07850];
(2) zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 = lutein + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07531]
Reaction(KEGG)
R07531 R07850
Substrate
alpha-carotene [CPD:C05433];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007];
zeinoxanthin [CPD:C08590]
Product
alpha-cryptoxanthin [CPD:C15981];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001];
lutein [CPD:C08601]
Comment
A cytochrome P-450 (heme-thiolate) protein.
History
EC 1.14.14.158 created 2011 as EC 1.14.99.45, transferred 2018 to EC 1.14.14.158
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09837  carotenoid epsilon hydroxylase
Genes
ATH: AT3G53130(LUT1)
ALY: 9313971
CRB: 17887227
CSAT: 104711869 104781166 104791575
EUS: EUTSA_v10010267mg EUTSA_v10023482mg
BRP: 103850280
BNA: 106352760
BOE: 106300296
RSZ: 108814454
THJ: 104818583
CPAP: 110822860
CIT: 102625870
PVY: 116143852
MINC: 123228821
TCC: 18598092
GRA: 105761792
GAB: 108470606
DZI: 111296450
EGR: 104438867
VRA: 106772401
VAR: 108326589
VUN: 114192824
CCAJ: 109818061
APRC: 113872166
LJA: Lj5g3v0681210.1(Lj5g3v0681210.1)
ADU: 107476248
AIP: 107626444
LANG: 109360988
FVE: 101308016
RCN: 112191404
PPER: 18771385
PMUM: 103339783
PAVI: 110747444
PDUL: 117635766
MDM: 103454736
ZJU: 107432039
MNT: 21405423
CSV: 101204961
CMO: 103500849(CHXE)
BHJ: 120073806
MCHA: 111013687
CMAX: 111471281
CMOS: 111463211
CPEP: 111782219
RCU: 8283943
JCU: 105642313
HBR: 110662670
MESC: 110620012
POP: 18100110
PEU: 105121750
PALZ: 118048373
QSU: 111984908
QLO: 115953099
TWL: 119985060
VVI: 100243621(LUT1)
SLY: 100322877(CYP97C11)
SPEN: 107002514
SOT: 102591274
CANN: 107843608
NSY: 104210223
NTO: 104114442
NAU: 109237187
INI: 109181096
ITR: 116006108
SIND: 105174233
OEU: 111404128
EGT: 105952850
SSPL: 121798854
HAN: 110882135
ECAD: 122581976
LSV: 111913167
CCAV: 112519301
DCR: 108220021
BVG: 104898583
SOE: 110788578
NNU: 104586192
MING: 122087098
PSOM: 113313399
NCOL: 116253613
OSA: 4349290
DOSA: Os10t0546600-01(Os10g0546600)
OBR: 102700091
BDI: 100835344
ATS: 109740072
SBI: 8058776
ZMA: 103637620
SITA: 101758095
PHAI: 112876006
PDA: 103719551
EGU: 105034264
MUS: 103970366
DCT: 110099518
PEQ: 110030448
AOF: 109825600
ATR: 18424541
PPP: 112283348
MNG: MNEG_1212
APRO: F751_6907
 » show all
Reference
1  [PMID:8837513]
  Authors
Pogson B, McDonald KA, Truong M, Britton G, DellaPenna D
  Title
Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants.
  Journal
Plant Cell 8:1627-39 (1996)
DOI:10.1105/tpc.8.9.1627
Reference
2  [PMID:14709673]
  Authors
Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D.
  Title
The Arabidopsis LUT1 locus encodes a member of the cytochrome p450 family that is required for carotenoid epsilon-ring hydroxylation activity.
  Journal
Proc Natl Acad Sci U S A 101:402-7 (2004)
DOI:10.1073/pnas.2237237100
  Sequence
[ath:AT3G53130]
Reference
3  [PMID:21450689]
  Authors
Stigliani AL, Giorio G, D'Ambrosio C
  Title
Characterization of P450 carotenoid beta- and epsilon-hydroxylases of tomato and  transcriptional regulation of xanthophyll biosynthesis in root, leaf, petal and fruit.
  Journal
Plant Cell Physiol 52:851-65 (2011)
DOI:10.1093/pcp/pcr037
  Sequence
[sly:100322877]
Reference
4  [PMID:26030746]
  Authors
Chang S, Berman J, Sheng Y, Wang Y, Capell T, Shi L, Ni X, Sandmann G, Christou P, Zhu C
  Title
Cloning and Functional Characterization of the Maize (Zea mays L.) Carotenoid Epsilon Hydroxylase Gene.
  Journal
PLoS One 10:e0128758 (2015)
DOI:10.1371/journal.pone.0128758
Reference
5  [PMID:28858245]
  Authors
Reddy CS, Lee SH, Yoon JS, Kim JK, Lee SW, Hur M, Koo SC, Meilan J, Lee WM, Jang JK, Hur Y, Park SU, Kim AYB
  Title
Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora.
  Journal
Molecules 22:E1449 (2017)
DOI:10.3390/molecules22091449
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.158
IUBMB Enzyme Nomenclature: 1.14.14.158
ExPASy - ENZYME nomenclature database: 1.14.14.158
BRENDA, the Enzyme Database: 1.14.14.158

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