KEGG   ENZYME: 1.14.14.159
Entry
EC 1.14.14.159              Enzyme                                 

Name
dolabradiene monooxygenase;
CYP71Z16 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
dolabradiene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3beta-hydroxy-15,16-epoxydolabrene-forming)
Reaction(IUBMB)
(1) dolabradiene + O2 + [reduced NADPH---hemoprotein reductase] = 15,16-epoxydolabrene + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R12224];
(2) 15,16-epoxydolabrene + O2 + [reduced NADPH---hemoprotein reductase] = 3beta-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R12225]
Reaction(KEGG)
R12224 R12225
Substrate
dolabradiene [CPD:C22008];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
15,16-epoxydolabrene [CPD:C22009]
Product
15,16-epoxydolabrene [CPD:C22009];
H2O [CPD:C00001];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
3beta-hydroxy-15,16-epoxydolabrene [CPD:C22010]
Comment
A cytochrome P-450 (heme thiolate) enzyme characterized from maize. The enzyme catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3.
History
EC 1.14.14.159 created 2018
Orthology
K23134  dolabradiene monooxygenase / zealexin A1 synthase
Genes
ZMA: 100273457 103628119
Reference
1  [PMID:29475898]
  Authors
Mafu S, Ding Y, Murphy KM, Yaacoobi O, Addison JB, Wang Q, Shen Z, Briggs SP, Bohlmann J, Castro-Falcon G, Hughes CC, Betsiashvili M, Huffaker A, Schmelz EA, Zerbe P
  Title
Discovery, Biosynthesis and Stress-Related Accumulation of Dolabradiene-Derived Defenses in Maize.
  Journal
Plant Physiol 176:2677-2690 (2018)
DOI:10.1104/pp.17.01351
  Sequence
[zma:103628119]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.159
IUBMB Enzyme Nomenclature: 1.14.14.159
ExPASy - ENZYME nomenclature database: 1.14.14.159
BRENDA, the Enzyme Database: 1.14.14.159

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