CYP82X1 (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine 8-hydroxylase,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (8-hydroxylating)
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + [reduced NADPH---hemoprotein reductase] + O2 = (13S,14R)-13-O-acetyl-1,8-dihydroxy-N-methylcanadine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
[reduced NADPH---hemoprotein reductase] [CPD:
[oxidized NADPH---hemoprotein reductase] [CPD:
This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
EC 22.214.171.124 created 2018
ec00950 Isoquinoline alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
K22096 (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine 8-hydroxylase
Dang TT, Chen X, Facchini PJ
Acetylation serves as a protective group in noscapine biosynthesis in opium poppy.
Li Y, Smolke CD
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.
Li Y, Li S, Thodey K, Trenchard I, Cravens A, Smolke CD
Complete biosynthesis of noscapine and halogenated alkaloids in yeast.